Catalog No.
EVV25101
Expression system
Mammalian Cells
Species
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Protein length
Arg30-Ser223
Predicted molecular weight
25 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P03205
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Glycoprotein 42, gp42, Soluble gp42, BZLF2
The structural basis of herpesvirus entry. PMID: 33087881
A bivalent Epstein-Barr virus vaccine induces neutralizing antibodies that block infection and confer immunity in humanized mice. PMID: 35507671
Antibody Generation and Immunogenicity Analysis of EBV gp42 N-Terminal Region. PMID: 34960650
Membrane anchoring of Epstein-Barr virus gp42 inhibits fusion with B cells even with increased flexibility allowed by engineered spacers. PMID: 25564465
Structural basis for Epstein-Barr virus host cell tropism mediated by gp42 and gHgL entry glycoproteins. PMID: 27929061
Mapping the N-terminal residues of Epstein-Barr virus gp42 that bind gH/gL by using fluorescence polarization and cell-based fusion assays. PMID: 20668073
Immunization with Components of the Viral Fusion Apparatus Elicits Antibodies That Neutralize Epstein-Barr Virus in B Cells and Epithelial Cells. PMID: 30979688
Binding-site interactions between Epstein-Barr virus fusion proteins gp42 and gH/gL reveal a peptide that inhibits both epithelial and B-cell membrane fusion. PMID: 17581996
Soluble Epstein-Barr virus glycoproteins gH, gL, and gp42 form a 1:1:1 stable complex that acts like soluble gp42 in B-cell fusion but not in epithelial cell fusion. PMID: 16973550
Characteristics of Epstein-Barr virus envelope protein gp42. PMID: 20162447
Investigation of the function of the putative self-association site of Epstein-Barr virus (EBV) glycoprotein 42 (gp42). PMID: 21550622
Structure of the Epstein-Barr virus gp42 protein bound to the MHC class II receptor HLA-DR1. PMID: 11864610
Epstein-Barr virus lacking glycoprotein gp42 can bind to B cells but is not able to infect. PMID: 9420211
Cleavage and secretion of Epstein-Barr virus glycoprotein 42 promote membrane fusion with B lymphocytes. PMID: 19369343
Epstein-Barr virus uses different complexes of glycoproteins gH and gL to infect B lymphocytes and epithelial cells. PMID: 9621012
Structural and Mechanistic Insights into the Tropism of Epstein-Barr Virus. PMID: 27094060
Epstein-Barr virus shed in saliva is high in B-cell-tropic glycoprotein gp42. PMID: 16809335
Inhibition of EBV-mediated membrane fusion by anti-gHgL antibodies. PMID: 28939750
The Cytoplasmic Tail Domain of Epstein-Barr Virus gH Regulates Membrane Fusion Activity through Altering gH Binding to gp42 and Epithelial Cell Attachment. PMID: 27935841
Human antibody titers to Epstein-Barr Virus (EBV) gp350 correlate with neutralization of infectivity better than antibody titers to EBV gp42 using a rapid flow cytometry-based EBV neutralization assay. PMID: 19584018
Mutational analysis of the HLA class II interaction with Epstein-Barr virus glycoprotein 42. PMID: 12805465
Potential entry receptors for human γ-herpesvirus into epithelial cells: A plausible therapeutic target for viral infections. PMID: 34800753
Integrins as triggers of Epstein-Barr virus fusion and epithelial cell infection. PMID: 21178476
Human MHC-II with Shared Epitope Motifs Are Optimal Epstein-Barr Virus Glycoprotein 42 Ligands-Relation to Rheumatoid Arthritis. PMID: 29361739
Structure of Epstein-Barr virus glycoprotein 42 suggests a mechanism for triggering receptor-activated virus entry. PMID: 19217393
Functional homology of gHs and gLs from EBV-related gamma-herpesviruses for EBV-induced membrane fusion. PMID: 17477951
Epstein-Barr virus evasion of CD8(+) and CD4(+) T cell immunity via concerted actions of multiple gene products. PMID: 18977445
[The entry of Epstein-Barr virus into B lymphocytes and epithelial cells during infection]. PMID: 25272606
Four Decades of Prophylactic EBV Vaccine Research: A Systematic Review and Historical Perspective. PMID: 35493498
The KGD motif of Epstein-Barr virus gH/gL is bifunctional, orchestrating infection of B cells and epithelial cells. PMID: 22215569
Proteins of purified Epstein-Barr virus. PMID: 15534216
Epstein-Barr virus gH is essential for penetration of B cells but also plays a role in attachment of virus to epithelial cells. PMID: 10864642
A novel high throughput assay to quantify Epstein-Barr virus neutralizing antibody activity against B-cell and epithelial cell infections for vaccine and therapeutic developments. PMID: 35577627
Sequence Variation of Epstein-Barr Virus: Viral Types, Geography, Codon Usage, and Diseases. PMID: 30111570
Assembly and architecture of the EBV B cell entry triggering complex. PMID: 25144748
Increased antibody levels to stage-specific Epstein-Barr virus antigens in systemic autoimmune diseases reveal a common pathology. PMID: 30727744
Immunoinformatic and systems biology approaches to predict and validate peptide vaccines against Epstein-Barr virus (EBV). PMID: 30679646
Association between Antibody Responses to Epstein-Barr Virus Glycoproteins, Neutralization of Infectivity, and the Risk of Nasopharyngeal Carcinoma. PMID: 33268566
An Antibody Targeting the Fusion Machinery Neutralizes Dual-Tropic Infection and Defines a Site of Vulnerability on Epstein-Barr Virus. PMID: 29669253
Functional analysis of glycoprotein L (gL) from rhesus lymphocryptovirus in Epstein-Barr virus-mediated cell fusion indicates a direct role of gL in gB-induced membrane fusion. PMID: 19457993
Compatibility of the gH homologues of Epstein-Barr virus and related lymphocryptoviruses. PMID: 17622614
Coreceptor restriction within the HLA-DQ locus for Epstein-Barr virus infection. PMID: 10908662
A soluble form of Epstein-Barr virus gH/gL inhibits EBV-induced membrane fusion and does not function in fusion. PMID: 23200314
Epstein-Barr virus uses HLA class II as a cofactor for infection of B lymphocytes. PMID: 9151859
Epstein-Barr Virus Fusion with Epithelial Cells Triggered by gB Is Restricted by a gL Glycosylation Site. PMID: 28956769
The large groove found in the gH/gL structure is an important functional domain for Epstein-Barr virus fusion. PMID: 23325693
Cholesterol-dependent infection of Burkitt's lymphoma cell lines by Epstein-Barr virus. PMID: 14573803
Epstein-Barr virus entry utilizing HLA-DP or HLA-DQ as a coreceptor. PMID: 10666279
A B-lymphocyte binding peptide from BNRF1 induced antibodies inhibiting EBV-invasion of B-lymphocytes. PMID: 15927339
Entry of herpesviruses into cells: more than one way to pull the trigger. PMID: 19217384
For research use only. Not for human or drug use.
