Description
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human IL6 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human IL6 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled Human IL6 antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Catalog No.
KHC15801
Applications
Used for the quantitative determination of Human IL6 concentration in serum, plasma and cell culture supernatant.
Stability and Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
Detection method
Colorimetric
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (pg/mL) |
158.0 |
36.3 |
9.0 |
153.5 |
34.1 |
8.9 |
|
Standard deviation |
13.3 |
3.4 |
0.5 |
16.6 |
3.3 |
1.0 |
|
CV (%) |
8.4 |
9.4 |
5.9 |
10.8 |
9.7 |
10.7 |
Sample type
Serum, Plasma and Cell culture supernatant.
Assay type
Quantitative
Sensitivity
2.37 pg/mL
Range
4.7 pg/mL - 300 pg/mL
Recovery
80-120%
Shipping
2-8 ℃
Note
For Research Use Only.
For research use only. Not for human or drug use.
