Catalog No.
PHD03401
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human MIF (Met1-Ala115).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
MIF,L-dopachrome isomerase,L-dopachrome tautomerase,GIF,Macrophage migration inhibitory factor,MMIF,Phenylpyruvate tautomerase,Glycosylation-inhibiting factor,GLIF
Purification
Purified by antigen affinity column.
Accession
P14174
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
CD36 regulates macrophage and endothelial cell activation and multinucleate giant cell formation in anti neutrophil cytoplasm antibody vasculitis., PMID:38286173
Lower serum AMH concentration is correlated with serum IgG1 decreased in the infertile woman: A real-world retrospective study., PMID:38286181
Targeting VEGF using Bevacizumab attenuates sepsis-induced liver injury in a mouse model of cecal ligation and puncture., PMID:38313162
Multiplex Immunofluorescence Staining Protocol for the Dual Imaging of Hypoxia-Inducible Factors 1 and 2 on Formalin-Fixed Paraffin-Embedded Samples., PMID:38319577
Granzyme B+ B cells detected by single-cell sequencing are associated with prognosis in patients with intrahepatic cholangiocarcinoma following liver transplantation., PMID:38386050
Single-cell and bulk RNA sequencing highlights the role of M1-like infiltrating macrophages in antibody-mediated rejection after kidney transplantation., PMID:38524599
Immunoglobulin-like transcript 2 as an impaired anti-tumor cytotoxicity marker of natural killer cells in patients with hepatocellular carcinoma., PMID:38638429
Targeting SIGLEC15 as an emerging immunotherapy for anaplastic thyroid cancer., PMID:38652971
MIF-Modulated Spinal Proteins Associated with Persistent Bladder Pain: A Proteomics Study., PMID:38674069
Comprehensive multi-omics analysis of resectable locally advanced gastric cancer: Assessing response to neoadjuvant camrelizumab and chemotherapy in a single-center, open-label, single-arm phase II trial., PMID:38685486
Infliximab modifies CD74-mediated lymphatic abnormalities and adipose tissue alterations in creeping fat of Crohn's disease., PMID:38713235
CXCL9 Overexpression Predicts Better HCC Response to Anti-PD-1 Therapy and Promotes N1 Polarization of Neutrophils., PMID:38737384
Chordoma cancer stem cell subpopulation characterization may guide targeted immunotherapy approaches to reduce disease recurrence., PMID:38741774
New-generation technologies for spatial tissue analysis, indispensable tools for deciphering intratumor heterogeneity in the development of antibody-drug conjugates and radio-immunoconjugates for cancer treatment., PMID:38751472
GammaGateR: semi-automated marker gating for single-cell multiplexed imaging., PMID:38833684
Risk of Seizure Recurrence Due to Autoimmune Encephalitis With NMDAR, LGI1, CASPR2, and GABABR Antibodies: Implications for Return to Driving., PMID:38838283
Immune profiling of mouse lung adenocarcinoma paraffin tissues using multiplex immunofluorescence panel: a pilot study., PMID:38877529
The role of macrophage migration inhibitory factor family and CD74 in the pathogenesis of melanoma., PMID:38884501
Detailed Profiling of the Tumor Microenvironment in Ethnic Breast Cancer, Using Tissue Microarrays and Multiplex Immunofluorescence., PMID:38928207
Ion channel profiles of extraocular motoneurons and internuclear neurons in human abducens and trochlear nuclei., PMID:38957435
Redox-dependent plasticity of oxMIF facilitates its interaction with CD74 and therapeutic antibodies., PMID:38972295
Antibiotic use attenuates response to immune checkpoint blockade in urothelial carcinoma via inhibiting CD74-MIF/COPA: revealing cross-talk between anti-bacterial immunity and ant-itumor immunity., PMID:38995167
Dynamics of scFv-targeted VAP2 correlating with IL-16, MIF and IL-1Ra in ANCA-associated vasculitis., PMID:39127096
Virtual multiplexed immunofluorescence staining from non-antibody-stained fluorescence imaging for gastric cancer prognosis., PMID:39154539
Development of a Multiplex Immunofluorescence Assay for Tumor Microenvironment Studies of Human and Murine Merkel Cell Carcinoma., PMID:39182611
Macrophage migration inhibitory factor as a therapeutic target in neuro-oncology: A review., PMID:39233830
Therapeutic Opportunities in Breast Cancer by Targeting Macrophage Migration Inhibitory Factor as a Pleiotropic Cytokine., PMID:39246383
Immunologic Crosstalk and Host-Specific Immune Signature Associated with Dengue., PMID:39281909
Application of Different Staining Methods in the Diagnosis of Pigment-Rich Melanoma., PMID:39315764
Analysis of the role of Dirofilaria repens macrophage migration inhibitory factors in host-parasite interactions., PMID:39318519
Enhancing diagnostic accuracy: Direct immunofluorescence assay as the gold standard for detecting Giardia duodenalis and Cryptosporidium spp. in canine and feline fecal samples., PMID:39358726
A Modified Bleaching Method for Multiplex Immunofluorescence Staining of FFPE Tissue Sections., PMID:39370592
Targeting of oxidized Macrophage Migration Inhibitory Factor (oxMIF) with antibody ON104 attenuates the severity of glomerulonephritis., PMID:39374239
CD34 as a potential prognostic indicator for camrelizumab response in advanced non-small-cell lung cancer: insights from digital spatial profiling., PMID:39429466
The VEGF-Mediated Cytoprotective Ability of MIF-Licensed Mesenchymal Stromal Cells in House Dust Mite-Induced Epithelial Damage., PMID:39502000
Methionine restriction promotes the polarization of macrophages towards M1 and the immunotherapy effect of PD-L1/PD-1 blockades by inhibiting the secretion of MIF by gastric carcinoma cells., PMID:39541710
Endothelial progenitor cell-derived conditioned medium mitigates chronic cerebral ischemic injury through macrophage migration inhibitory factor-activated AKT pathway., PMID:39543689
Autofluorescence Virtual Staining System for H&E Histology and Multiplex Immunofluorescence Applied to Immuno-Oncology Biomarkers in Lung Cancer., PMID:39636222
Serum cytokines as a biomarker for immune checkpoint inhibitor toxicity in patients with pleural mesothelioma., PMID:39687621
Plasma protein dynamics during ipilimumab treatment in metastatic melanoma: associations with tumor response, adverse events and survival., PMID:39703053
Integrating In Silico and In Vitro Tools for Optimized Antibody Development-Design of Therapeutic Anti-oxMIF Antibodies., PMID:39727487
System vaccinology analysis of predictors and mechanisms of antibody response durability to multiple vaccines in humans., PMID:39747435
Exosome-Mediated Lectin Pathway and Resistin-MIF-AA Metabolism Axis Drive Immune Dysfunction in Immune Thrombocytopenia., PMID:39792656
Single-Cell RNA Sequencing Reveals Macrophage Dynamics During MASH in Leptin-Deficient Rats., PMID:39851524
Pregnant Women Chronically Infected by Toxoplasma gondii with Depressive Disorder: Differential Modulation of Pro-Inflammatory and Anti-Inflammatory Cytokines., PMID:40333139
Inhibition of MIF with an Allosteric Inhibitor Triggers Cell Cycle Arrest in Acute Myeloid Leukemia., PMID:40352549
Novel function of macrophage migration inhibitory factor in regulating post-infarct inflammation and the therapeutic significance., PMID:40383291
MIF regulates T helper 17 cell differentiation by activating the p38 MAPK signaling pathway to drive the pathogenesis of EAP., PMID:40440956
Multi-omics unveils BCAA metabolism markers L-leucine and HMGCS1 as prognostic marker for immunotherapy efficacy in non-small cell lung cancer., PMID:40457349
CX3CR1+ Monocytes/Macrophages Promote Regional Immune Injury in Mesangial Proliferative Glomerulonephritis through Crosstalk with Activated Mesangial Cells., PMID:40458609
For research use only. Not for human or drug use.
