Catalog No.
PHK44101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CDC6 (Lys151-Pro560).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
HsCdc18, CDC18L, CDC6, HsCDC6, CDC6-related protein, Cell division control protein 6 homolog, p62(cdc6), Cdc18-related protein
Purification
Purified by antigen affinity column.
Accession
Q99741
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Significance of DNA Replication Licensing Proteins (MCM2, MCM5 and CDC6), p16 and p63 as Markers of Premalignant Lesions of the Uterine Cervix: Its Usefulness to Predict Malignant Potential., PMID:29373905
Cell Division Cycle 6 Promotes Mitotic Slippage and Contributes to Drug Resistance in Paclitaxel-Treated Cancer Cells., PMID:27611665
A Role of hIPI3 in DNA Replication Licensing in Human Cells., PMID:27057756
Targeting of cytosolic phospholipase A2α impedes cell cycle re-entry of quiescent prostate cancer cells., PMID:26416244
Cyclin-A-CDK2-mediated phosphorylation of CIZ1 blocks replisome formation and initiation of mammalian DNA replication., PMID:25736292
Phosphorylation of Cdc6 at serine 74, but not at serine 106, drives translocation of Cdc6 to the cytoplasm., PMID:23129444
Cdc45 limits replicon usage from a low density of preRCs in mammalian cells., PMID:21390258
ARRB1-mediated regulation of E2F target genes in nicotine-induced growth of lung tumors., PMID:21212384
Replication origins are already licensed in G1 arrested unfertilized sea urchin eggs., PMID:20153311
Increased origin activity in transformed versus normal cells: identification of novel protein players involved in DNA replication and cellular transformation., PMID:20064876
Low expression of a few genes indicates good prognosis in estrogen receptor positive breast cancer., PMID:19619298
The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells., PMID:18320024
Genome-wide analysis of mouse myeloma cell lines expressing therapeutic antibodies., PMID:17592857
Identification and functional analysis of a human homologue of the monkey replication origin ors8., PMID:16823771
p16INK4A, CDC6, and MCM5: predictive biomarkers in cervical preinvasive neoplasia and cervical cancer., PMID:15858126
Recombinant Cdt1 induces rereplication of G2 nuclei in Xenopus egg extracts., PMID:15668171
Overexpression of the replication licensing regulators hCdt1 and hCdc6 characterizes a subset of non-small-cell lung carcinomas: synergistic effect with mutant p53 on tumor growth and chromosomal instability--evidence of E2F-1 transcriptional control over hCdt1., PMID:15466399
Cdc6 chromatin affinity is unaffected by serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A., PMID:14749377
Immunohistochemical localization of cdc6 in squamous and glandular neoplasia of the uterine cervix., PMID:12296751
A key role of the hSNF5/INI1 tumour suppressor in the control of the G1-S transition of the cell cycle., PMID:12226744
Essential role of human CDT1 in DNA replication and chromatin licensing., PMID:11896191
Analysis of Cdc6 function in the assembly of mammalian prereplication complexes., PMID:11805305
Cdc6 expression as a marker of proliferative activity in brain tumors., PMID:11496317
DNA replication licensing and human cell proliferation., PMID:11493639
Assembly of the human origin recognition complex., PMID:11323433
Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis., PMID:11046155
Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1., PMID:10995389
CDC6 interacts with c-Myc to inhibit E-box-dependent transcription by abrogating c-Myc/Max complex., PMID:10899308
Identification and characterization of Saccharomyces cerevisiae Cdc6 DNA-binding properties., PMID:10793143
Interaction of Xenopus Cdc2 x cyclin A1 with the origin recognition complex., PMID:10660590
DNA replication in quiescent cell nuclei: regulation by the nuclear envelope and chromatin structure., PMID:10588645
Cyclin A-dependent kinase activity affects chromatin binding of ORC, Cdc6, and MCM in egg extracts of Xenopus laevis., PMID:10491086
Minichromosome maintenance proteins as biological markers of dysplasia and malignancy., PMID:10473096
Multistep regulation of DNA replication by Cdk phosphorylation of HsCdc6., PMID:10339564
Molecular changes herald a new era for cervical cancer screening., PMID:10322308
Improved cervical smear assessment using antibodies against proteins that regulate DNA replication., PMID:9843993
The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p., PMID:9829972
Cell cycle-regulated expression of mammalian CDC6 is dependent on E2F., PMID:9774682
Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells., PMID:9520412
Is the yeast anaphase promoting complex needed to prevent re-replication during G2 and M phases?, PMID:9312056
Negative regulation of G1 and G2 by S-phase cyclins of Saccharomyces cerevisiae., PMID:7651421
For research use only. Not for human or drug use.
