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Anti-HSPA1A Polyclonal Antibody

Catalog #:   PHC69301 Specific References (29) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P0DMV8
Overview

Catalog No.

PHC69301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPA1A (Met1-Asp641).

Applications

ELISA, IHC, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

HSP70.1,HSP70-1,HSPA1,HSPA1A,Heat shock 70 kDa protein 1A,Heat shock 70 kDa protein 1,HSP72,HSX70

Purification

Purified by antigen affinity column.

Accession

P0DMV8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HSPA1A antibody (PHC69301) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 72 kDa
    Observed MW: 72 kDa
References

Pharmacological induction of heat shock protein 68 synthesis in cultured rat astrocytes., PMID:8530397

Comparison of anti-heat shock protein 70 (anti-hsp70) and anti-68-kDa inner ear protein in the sera of patients with Meniere's disease., PMID:9023247

Association analysis of heat shock protein 70 gene polymorphisms in schizophrenia., PMID:18299791

The equatorial subsegment in mammalian spermatozoa is enriched in tyrosine phosphorylated proteins., PMID:18448843

HspBP1 levels are elevated in breast tumor tissue and inversely related to tumor aggressiveness., PMID:18987994

Extracellular heat shock protein 70 (HSPA1A) and classical vascular risk factors in a general population., PMID:20490736

Comment to: Humanization of a mouse monoclonal antibody directed against a cell surface-exposed epitope of membrane-associated heat shock protein 70 (Hsp70)., PMID:20668964

Distinguishing integral and receptor-bound heat shock protein 70 (Hsp70) on the cell surface by Hsp70-specific antibodies., PMID:21165727

Variants of HSPA1A in combination with plasma Hsp70 and anti-Hsp70 antibody levels associated with higher risk of acute coronary syndrome., PMID:21849784

Measuring Hsp72 (HSPA1A) by indirect sandwich ELISA., PMID:21898234

Cellular protein quality control and the evolution of aggregates in spinocerebellar ataxia type 3 (SCA3)., PMID:21916928

The effects of fever on hormone ghrelins, immunoglobulins, and heat shock protein 70 expression after swine flu vaccinations., PMID:22477150

Amphibian oocytes release heat shock protein A during spawning: evidence for a role in fertilization., PMID:22623622

Transcriptome profiling and genome-wide DNA binding define the differential role of fenretinide and all-trans RA in regulating the death and survival of human hepatocellular carcinoma Huh7 cells., PMID:23396089

Reactive γ-ketoaldehydes promote protein misfolding and preamyloid oligomer formation in rapidly-activated atrial cells., PMID:25463275

Early inflammatory response in polytraumatized patients: Cytokines and heat shock proteins. A pilot study., PMID:26068807

Hsp70 (HSPA1) Lysine Methylation Status as a Potential Prognostic Factor in Metastatic High-Grade Serous Carcinoma., PMID:26448330

[Role of heat shock proteins in the cardioprotection of regular moderate alcohol consumption]., PMID:26902796

Systematic protein-protein interaction and pathway analyses in the idiopathic inflammatory myopathies., PMID:27388770

Molecular markers of systemic autoimmune disorders: the expression of MHC-located HSP70 genes is significantly associated with autoimmunity development., PMID:28032847

Overexpression of cytosolic, plasma membrane bound and extracellular heat shock protein 70 (Hsp70) in primary glioblastomas., PMID:28849427

HSP70 Facilitates Memory Consolidation of Fear Conditioning through MAPK Pathway in the Hippocampus., PMID:29374537

Dual-platform affinity proteomics identifies links between the recurrence of ovarian carcinoma and proteins released into the tumor microenvironment., PMID:31588238

Time- and Dose-Dependent Effects of Ionizing Irradiation on the Membrane Expression of Hsp70 on Glioma Cells., PMID:32276468

FHL-1 interacts with human RPE cells through the α5β1 integrin and confers protection against oxidative stress., PMID:34239032

Integrative system biology and mathematical modeling of genetic networks identifies shared biomarkers for obesity and diabetes., PMID:35240786

Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infection., PMID:37434783

Comprehensive multiplexed autoantibody profiling of patients with advanced urothelial cancer., PMID:38309723

Single-cell transcriptomic analysis reveals distinct plasma cell populations in chronic thromboembolic pulmonary hypertension., PMID:39965671

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-HSPA1A Polyclonal Antibody [PHC69301]
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