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Anti-HSPA1A Polyclonal Antibody (PHC69301)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P0DMV8
Overview

Catalog No.

PHC69301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPA1A (Met1-Asp641).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

HSP70.1,HSP70-1,HSPA1,HSPA1A,Heat shock 70 kDa protein 1A,Heat shock 70 kDa protein 1,HSP72,HSX70

Purification

Purified by antigen affinity column.

Accession

P0DMV8

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with HSPA1A antibody (PHC69301) at 0.42μg/ml.

    Lane 1: 293T cell lysate
    Lane 2: A549 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: A2780 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 70 kDa
    Observed MW: 70 kDa
  • Immunocytochemistry/ Immunofluorescence
    HSPA1A in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with HSPA1A (PHC69301) at 8.4 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-HSPA1A Polyclonal Antibody [PHC69301]
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