Catalog No.
PHC69301
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human HSPA1A (Met1-Asp641).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
HSP70.1,HSP70-1,HSPA1,HSPA1A,Heat shock 70 kDa protein 1A,Heat shock 70 kDa protein 1,HSP72,HSX70
Purification
Purified by antigen affinity column.
Accession
P0DMV8
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Pharmacological induction of heat shock protein 68 synthesis in cultured rat astrocytes., PMID:8530397
Comparison of anti-heat shock protein 70 (anti-hsp70) and anti-68-kDa inner ear protein in the sera of patients with Meniere's disease., PMID:9023247
Association analysis of heat shock protein 70 gene polymorphisms in schizophrenia., PMID:18299791
The equatorial subsegment in mammalian spermatozoa is enriched in tyrosine phosphorylated proteins., PMID:18448843
HspBP1 levels are elevated in breast tumor tissue and inversely related to tumor aggressiveness., PMID:18987994
Extracellular heat shock protein 70 (HSPA1A) and classical vascular risk factors in a general population., PMID:20490736
Comment to: Humanization of a mouse monoclonal antibody directed against a cell surface-exposed epitope of membrane-associated heat shock protein 70 (Hsp70)., PMID:20668964
Distinguishing integral and receptor-bound heat shock protein 70 (Hsp70) on the cell surface by Hsp70-specific antibodies., PMID:21165727
Variants of HSPA1A in combination with plasma Hsp70 and anti-Hsp70 antibody levels associated with higher risk of acute coronary syndrome., PMID:21849784
Measuring Hsp72 (HSPA1A) by indirect sandwich ELISA., PMID:21898234
Cellular protein quality control and the evolution of aggregates in spinocerebellar ataxia type 3 (SCA3)., PMID:21916928
The effects of fever on hormone ghrelins, immunoglobulins, and heat shock protein 70 expression after swine flu vaccinations., PMID:22477150
Amphibian oocytes release heat shock protein A during spawning: evidence for a role in fertilization., PMID:22623622
Transcriptome profiling and genome-wide DNA binding define the differential role of fenretinide and all-trans RA in regulating the death and survival of human hepatocellular carcinoma Huh7 cells., PMID:23396089
Reactive γ-ketoaldehydes promote protein misfolding and preamyloid oligomer formation in rapidly-activated atrial cells., PMID:25463275
Early inflammatory response in polytraumatized patients: Cytokines and heat shock proteins. A pilot study., PMID:26068807
Hsp70 (HSPA1) Lysine Methylation Status as a Potential Prognostic Factor in Metastatic High-Grade Serous Carcinoma., PMID:26448330
[Role of heat shock proteins in the cardioprotection of regular moderate alcohol consumption]., PMID:26902796
Systematic protein-protein interaction and pathway analyses in the idiopathic inflammatory myopathies., PMID:27388770
Molecular markers of systemic autoimmune disorders: the expression of MHC-located HSP70 genes is significantly associated with autoimmunity development., PMID:28032847
Overexpression of cytosolic, plasma membrane bound and extracellular heat shock protein 70 (Hsp70) in primary glioblastomas., PMID:28849427
HSP70 Facilitates Memory Consolidation of Fear Conditioning through MAPK Pathway in the Hippocampus., PMID:29374537
Dual-platform affinity proteomics identifies links between the recurrence of ovarian carcinoma and proteins released into the tumor microenvironment., PMID:31588238
Time- and Dose-Dependent Effects of Ionizing Irradiation on the Membrane Expression of Hsp70 on Glioma Cells., PMID:32276468
FHL-1 interacts with human RPE cells through the α5β1 integrin and confers protection against oxidative stress., PMID:34239032
Integrative system biology and mathematical modeling of genetic networks identifies shared biomarkers for obesity and diabetes., PMID:35240786
Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infection., PMID:37434783
Comprehensive multiplexed autoantibody profiling of patients with advanced urothelial cancer., PMID:38309723
Single-cell transcriptomic analysis reveals distinct plasma cell populations in chronic thromboembolic pulmonary hypertension., PMID:39965671