Catalog No.
YBC05501
Description
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. It catalyzes the repeated addition of deoxyribonucleotides to oligodeoxyribonucleotides and 3' -Oh of single - and double-stranded DNA. TdT requires an oligodeoxynucleotide of at least three bases as a primer. The addition of Co2+ in the reacton makes tailing more efficient.
Species
Bovine
Protein length
Terminal Transferase (TdT) is expressed in E. coli.
Predicted molecular weight
45 kDa
Nature
Recombinant
Concentration
0.9 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Applications
Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
Form
Liquid
Storage buffer
100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Alternative Names
DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT
Note
For research use only.
Jun N-Terminal Kinase Inhibitor Suppresses CASK Deficiency-Induced Cerebellar Granular Cell Death in MICPCH Syndrome Model Mice., PMID:40422253
BLF1 Affects ATP Hydrolysis Catalyzed by Native and Mutated eIF4A1 and eIF4A2 Proteins., PMID:40423315
High-affinity detection of biotinylated endogenous neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse., PMID:40424132
Ophiopogonin D protects against cerebral ischemia-reperfusion injury in rats by inhibiting STAT3 phosphorylation., PMID:40424636
Engineering of ω-transaminase at binding pocket and access tunnel for preparation of (R)-1-phenoxypropan-2-amine., PMID:40424941
Ephrin-A5 or EphA7 stimulation is anti-proliferative for human rhabdomyosarcoma in vitro., PMID:40426233
Noncanonical Poly(A) Polymerase TENT4 Drives Expression of Subgenomic Hepatitis A Virus RNAs in Infected Cells., PMID:40431677
A Model of Non-Homologous Recombination Mediated by HIV-1 Reverse Transcriptase Explaining Sequence Motif Duplications That Confer a Replication Fitness Advantage., PMID:40431692
TAR RNA Mimicry of INI1 and Its Influence on Non-Integration Function of HIV-1 Integrase., PMID:40431704
Structural basis for substrate selectivity and evolutionary insights into human choline phosphotransferase 1., PMID:40435706
The distinct effect between Amyloid β (1‒40) and Amyloid β (1‒42) on the TRAP-stimulated platelet activation in diabetes mellitus., PMID:40436763
CEACAM1 as a mediator of B-cell receptor signaling in mantle cell lymphoma., PMID:40436855
NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation., PMID:40436859
Nucleoporins cooperate with Polycomb silencers to promote transcriptional repression and repair at DNA double-strand breaks., PMID:40440073
The YoaA-χ helicase modulates the dynamics of single-stranded DNA binding protein on DNA., PMID:40442115
Platelet aggregation elicits FasL expression and hepatocyte apoptosis in sinusoidal obstruction syndrome., PMID:40442395
Apurinic/apyrimidinic endonuclease 1/redox factor-1 deficiency exacerbates renal fibrosis in a unilateral ureteral obstruction model., PMID:40443303
Granzyme K, Regulated by the N6-Methyladenosine Methyltransferase Wilms' tumor 1-associate protein, Enhances Myocardial Infarction Injury., PMID:40445733
Structural insights into sigma class glutathione transferase from Taenia solium: Analysis and functional implications., PMID:40445974
Linking expression and function of Drosophila type-I TGF-β receptor baboon isoforms: Multiple roles of BaboA isoform in shaping of the adult central nervous system., PMID:40445987
Differential phosphorylation of receptor kinase SlLYK4 mediates immune responses to bacterial and fungal pathogens in tomato., PMID:40446045
Sequential structure probing of cotranscriptional RNA folding intermediates., PMID:40450030
Artesunate alleviates cerebral ischemia/reperfusion injury by suppressing FUNDC1-mediated excessive mitophagy., PMID:40451544
PRMT4/CARM1 Is a Novel Factor Promoting DNA Double-Strand Break Repair., PMID:40452456
Gold Nanoparticle-DNA Conjugates: An Enzymatic DNA Synthesis Platform., PMID:40454042
Myocardial mitochondrial antiviral signaling protein promotes heart Ischemia-reperfusion injury via RIG-I signaling in mice., PMID:40456736
Hampered AMPK-ULK1 cascade in Alzheimer's disease (AD) instigates mitochondria dysfunctions and AD-related alterations which are alleviated by metformin., PMID:40457477
[Mechanism of Xiangsha Liujunzi Decoction in improving autophagy in interstitial cells of Cajal of rats with functional dyspepsia by regulation of IRE1/ASK1/JNK pathway]., PMID:40461233
Toxicity analysis of gold nanoparticle formulations used for intraocular applications., PMID:40467755
Trem2 regulates microglial migratory responses via type I interferon signaling during photoreceptor degeneration., PMID:40468324
Rhein-induced apoptosis in colorectal cancer cell lines: A mechanistic study of the myeloid differentiation primary response gene 88/toll-like receptor 4/nuclear factor kappa-B signaling pathway., PMID:40469706
Zinc finger and broad-complex, tramtrack, and bric-a-brac domain containing 16 silencing attenuates bleomycin-induced pulmonary fibrosis in mice through inhibition of the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin pathway., PMID:40469708
NAD+ Metabolism Maintains the Homeostasis of Decidual Macrophages in Early Pregnancy., PMID:40471104
Insights into the functional characteristics of rhubarb (Rheum officinale Baill) treatment on experimental traumatic brain injury through network pharmacology with metagenomics., PMID:40472396
Investigation of HO-1 Regulation of Liver Fibrosis Related to Nonalcoholic Fatty Liver Disease Through the SIRT1/TGF-ß/Smad3 Pathway., PMID:40474887
Biosecurity Primitive: Polymerase X-based Genetic Physical Unclonable Functions., PMID:40485605
Cell cycle proteins: Linking the cell cycle to tumors., PMID:40486867
Apatinib modulates sorafenib-resistant hepatocellular carcinoma through inhibiting the EGFR/JNK/ERK signaling pathway., PMID:40486881
Dopamine attenuates ethanol-induced neuronal apoptosis by stimulating electrical activity in the developing rat retina., PMID:40486926
The functionality of telomerase depends on CPF-CF induced 3'end processing of its RNA component TLC1 and a novel Nrd1-Nab3 surveillance mechanism., PMID:40488281
Exploring structure-activity relationships of pyrrolyl diketo acid derivatives as non-nucleoside inhibitors of terminal deoxynucleotidyl transferase enzyme., PMID:40488680
GPCR kinases phosphorylate GPCR C-terminal peptides in a hierarchical manner., PMID:40490497
Asymmetric loading of TnsE regulates Tn7 targeting of DNA replication structures., PMID:40498074
KIF2C condensation concentrates PLK1 and phosphorylated BRCA2 on kinetochore microtubules in mitosis., PMID:40498077
OTUD1 exacerbates sepsis-associated encephalopathy by promoting HK2 mitochondrial release to drive microglia pyroptosis., PMID:40500776
MAPK15 Prevents IFNB1 Expression by Suppressing Oxidative Stress-Dependent Activation of the JNK-JUN Pathway., PMID:40507959
Frequency-Dependent Premature Differentiation of Pheochromocytoma Cells Exhibits Band-Pass Filter Behavior Correlation with Intracellular Enzyme Activation Kinetics., PMID:40508091
Regulation of Blood-Brain Barrier Permeability via JNK Signaling Pathway: Mechanisms and Potential Therapeutic Strategies for Ischemic Stroke, Alzheimer's Disease and Brain Tumors., PMID:40509244
Baicalin alleviates sepsis-associated acute kidney injury through activation of the PPAR-γ/UCP1 signaling pathway., PMID:40509797
Dry period heat tolerance affects placental efficiency, apoptosis, and protein expression in Holstein cows., PMID:40513876
For research use only. Not for human or drug use.
