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Anti-IL6 Polyclonal Antibody

Catalog #:   PHC15801 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, IF, WB
Accession: P05231
Overview

Catalog No.

PHC15801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IL6 (Val30-Met212).

Applications

ELISA, IHC, IF, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:100, IF:1:50-1:100, WB: 1:500-1:4000

Target

Hybridoma growth factor,BSF-2,IFN-beta-2,CDF,IL6,Interleukin-6,IL-6,B-cell stimulatory factor 2,Interferon beta-2,IFNB2,CTL differentiation factor

Concentration

0.36 mg/ml

Purification

Purified by antigen affinity column.

Accession

P05231

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with IL6 antibody (PHC15801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 17 kDa
    Observed MW: 17 kDa
  • Immunocytochemistry/ Immunofluorescence
    IL6 in U251 Cell Line.
    The U251 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with IL6 (PHC15801) at 7.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Dual therapy for amanita phalloides-induced acute liver failure in mice: A combination of etanercept and alpha-1 antitrypsin., PMID:40398510

[Update of clinical management in autoimmune encephalitis-2004]., PMID:40399061

Effects of infliximab infusion on clinical symptom scores and serum cytokines in patients with inflammatory bowel disease., PMID:40400059

Evaluation of humoral and cellular immune responses in healthcare workers with varying levels of SARS-CoV-2 exposure: effects of CoronaVac vaccination followed by heterologous booster., PMID:40406109

Immunological response and implications of Ad26.COV2. S (Janssen COVID-19 vaccine) vaccine in diabetic patients: a prospective cohort study in Ethiopia., PMID:40406137

Macrophage activation syndrome associated with neonatal lupus erythematosus., PMID:40407016

A Handheld Saliva Analyzer Employing Homogeneous Nanogrid Film for Noninvasive on-Site Detection Periodontitis and Salivary Gland Tumor., PMID:40411872

Effects of TGMXD-208, a Novel PI3K Inhibitor, on Adjuvant-induced Arthritic Rats by Suppressing PI3K/AKT Signaling Pathway., PMID:40415316

Investigating Anti-Tumor Effects of Irisin in Cervical Cancer Cells: Cell Viability, Migration, and Tumor-Associated Macrophage Polarization., PMID:40415369

[Protective аction of ginseng root extract on myofibril apoptosis and immune response in rats after exhausting physical exercise. Part II. Effect of ginseng root extract on immunity and regulation of energy metabolism]., PMID:40418135

Phase 1b/2 study of ADG106, a 4-1BB/CD137 agonist, in combination with toripalimab in patients with advanced solid tumors., PMID:40421181

Interactions of Probiotics with the Immune Cells of Patients with COVID-19 Pneumonia., PMID:40424990

Immunocytokines and cytokine neutralization for cancer immunotherapy., PMID:40425444

C1q Binds to CD4+ T Cells and Inhibits the Release of Pro-Inflammatory Cytokines: Role in the Pathogenesis of Systemic Lupus Erythematosus., PMID:40429614

OVA-Induced Food Allergy Leads to Neurobehavioral Changes in Mice and the Potential Role of Gut Microbiota and Metabolites Dysbiosis., PMID:40429903

A Modular Mathematical Model of the Immune Response for Investigating the Pathogenesis of Infectious Diseases., PMID:40431602

A case of Schnitzler syndrome complicated by rheumatoid arthritis treated with methotrexate., PMID:40433863

IL-6 Inhibitors and TNF Inhibitors: Impact on Exercise-induced Cardiac Adaptations in Patients With Rheumatoid Arthritis., PMID:40436519

Itaconate derivative 4-OI inhibits M1 macrophage polarization and restores its impaired function in immune thrombocytopenia through metabolic reprogramming., PMID:40437667

Antibody-mediated Rejection - Treatment Standard., PMID:40440205

Immunostimulatory effects of multiple short-hairpin RNAs enhance foot-and-mouth disease vaccine-induced humoral immunity., PMID:40441607

Epstein-Barr virus infection increases the risk of depression: A cross-sectional study and Mendelian randomization analysis., PMID:40441653

Differences in Aqueous Humor Cytokine Concentrations According to the Presence of Posterior Subcapsular Cataract: A Prospective Case-Control Study., PMID:40441712

Soil-Transmitted Helminths and the Intricacies of Immunoregulation: Evidence From Amazonian Ecuador for the Importance of Considering Species-Specific Effects Within the Old Friends Hypothesis., PMID:40444920

The recombinant anti-MET/EpCAM bispecific antibody fragment: a promising novel therapeutic approach for breast cancer treatment., PMID:40445574

Immune responses and recovery from spotty liver disease in layer birds., PMID:40446677

Multi-omics analysis reveals Angelica sinensis-Carthamus tinctorius herb pair ameliorates diabetic retinopathy comorbid with depressive symptoms via the gut-eye-brain axis., PMID:40449450

A Spanish Porcine Reproductive and Respiratory Syndrome Virus 1 Strain Is Highly Virulent in Pigs Under Experimental Conditions., PMID:40453458

Longitudinal analysis of cytokine dynamics in severe fever with thrombocytopenia syndrome patients - High-incidence regions of China (2010-2023)., PMID:40453472

The role of IGF-2 and its variants in enhancing endothelial migration and angiogenesis., PMID:40454313

Several Common Genetic Variations Associate With Functional or Anatomic Effects of Anti-VEGF Treatment in Conditions With Macular Edema., PMID:40455044

Toll-like receptor engaged in activation and osteoimmuno-cytokines production by unswitched memory B cells during periodontitis., PMID:40455294

Alternation of anti-NMDA receptor subunit GluN2 antibody in a patient with SLE who promptly developed anxiety after belimumab., PMID:40455606

IL-6: a pivotal molecule for tumor-brain dysfunction interaction., PMID:40461299

RNase 7 and Th cytokines synergistically increase the secretion of interleukin-6 from keratinocytes., PMID:40461723

Novel Aptamers Targeting Sclerostin Loop3 Improve Skeletal and Muscle Properties Without Adverse Cardiovascular Effects in Orchiectomized Mice., PMID:40464222

[Correlation between soluble CD146 and systemic vasculitis]., PMID:40484821

Adaptive immune changes in colorectal cancer: a focus on T and B cell activation genes., PMID:40484866

The role of cytokines in pediatric hematologic malignancies: mechanisms of tumor progression and therapeutic implications - a narrative review., PMID:40486628

Activin A exacerbates neonatal necrotizing enterocolitis via ALK4-mediated apoptosis and barrier disruption., PMID:40494204

Effects of Rhodobacter sphaeroides protein on growth performance, nutrient digestibility, rumen fermentation, and serum metabolites in dairy calves during the pre- and postweaning periods., PMID:40499903

Adipose-Derived Mesenchymal Stromal/Stem Cells Reduce Inflammatory Responses and Partially Alleviate Skin Symptoms in Imiquimod-Induced Psoriasis-Like Dermatitis Model Mice., PMID:40500215

Chronic administration of morphine provokes generation of antibodies to morphine and immunosuppression in individuals with opioid use disorder., PMID:40501089

Inflammatory arthritis immune related adverse events represent a unique autoimmune disease entity primarily driven by T cells, but likely not autoantibodies., PMID:40502578

CTRP7 as a molecular biomarker associating with responsiveness to pulmonary vasodilators: insights from human and animal studies in pulmonary arterial hypertension., PMID:40504501

FcRn inhibitors in immune thrombocytopenia: A comprehensive review of therapeutic advances and clinical outcomes., PMID:40505332

Anti-Tumor Activities of Anti-Siglec-15 Chimeric Heavy-Chain Antibodies., PMID:40507880

A Mixture of Free and Microencapsulated Essential Oils Combined with Turmeric and Tannin in the Diet of Dairy Cows: Effects on Productive Efficiency and Animal Health., PMID:40509055

Role of Ocrelizumab in modulating gene and microRNA expression in multiple sclerosis., PMID:40510879

TNFα signaling restores steady-state hematopoiesis in a TNFαKO mouse model of anemia of inflammation., PMID:40512402

Datasheet

Document Download

Anti-IL6 Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-IL6 Polyclonal Antibody [PHC15801]
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