Anti-ESR1/ER-alpha Polyclonal Antibody (PHC03201)

specific references (0) DATASHEET

Host species：Rabbit

Isotype：IgG

Applications：ELISA, IHC, WB

Accession：P03372

Overview

Catalog No.

PHC03201

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ESR1/ER-alpha (Met297-Ser554).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Estrogen receptor,ER-alpha,ESR,ER,ESR1,Nuclear receptor subfamily 3 group A member 1,NR3A1,Estradiol receptor

Purification

Purified by antigen affinity column.

Accession

P03372

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image

WESTERN BLOT

Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ESR1/ER-alpha antibody (PHC03201) at 1 μg/ml.

Lane 1: Recombinant protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 32 kDa
Observed MW: 32 kDa
Immunocytochemistry/ Immunofluorescence

ESR1 / ER-alpha in MDA-MB-231 Cell Line.
The MDA-MB-231 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ESR1 / ER-alpha (PHC03201) at 17.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
Immunocytochemistry/ Immunofluorescence

ESR1 / ER-alpha in SKOV3 Cell Line.
The SKOV3 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ESR1 / ER-alpha (PHC03201) at 17.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).

References

Datasheet

Document Download

Anti-ESR1/ER-alpha Polyclonal Antibody.pdf

$ 170

Product specifications

50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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