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Anti-ESR1/ER-alpha Polyclonal Antibody (PHC03201)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P03372
Overview

Catalog No.

PHC03201

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ESR1/ER-alpha (Met297-Ser554).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Estrogen receptor,ER-alpha,ESR,ER,ESR1,Nuclear receptor subfamily 3 group A member 1,NR3A1,Estradiol receptor

Purification

Purified by antigen affinity column.

Accession

P03372

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ESR1/ER-alpha antibody (PHC03201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 32 kDa
    Observed MW: 32 kDa
  • Immunocytochemistry/ Immunofluorescence
    ESR1 / ER-alpha in MDA-MB-231 Cell Line.
    The MDA-MB-231 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ESR1 / ER-alpha (PHC03201) at 17.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Immunocytochemistry/ Immunofluorescence
    ESR1 / ER-alpha in SKOV3 Cell Line.
    The SKOV3 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ESR1 / ER-alpha (PHC03201) at 17.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-ESR1/ER-alpha Polyclonal Antibody [PHC03201]
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