Catalog No.
PHC01801
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PF4/CXCL4 (Glu32-Ser101).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
C-X-C motif chemokine 4,PF-4,Oncostatin-A,Endothelial cell growth inhibitor,CXCL4,Iroplact,Platelet factor 4,PF4,SCYB4
Purification
Purified by antigen affinity column.
Accession
P02776
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Antithrombotic efficacy and bleeding risks of vaccine-induced immune thrombotic thrombocytopenia treatments., PMID:39293086
Type 2-like polarization and elevated CXCL4 secretion of monocyte derived macrophages upon internalization of plasma-derived exosomes from head and neck cancer patients., PMID:39304856
A novel role for thioredoxin-related transmembrane protein TMX4 in platelet activation and thrombus formation., PMID:39307246
Subsequent Vaccination against SARS-CoV-2 after Vaccine-Induced Immune Thrombotic Thrombocytopenia., PMID:39336949
[Management of HIT/anti-PF4 disorders]., PMID:39358268
Autoantibodies to protein S may explain rare cases of coagulopathy following COVID-19 vaccination., PMID:39424883
Is the combination of two automated rapid assays for diagnosis of heparin-induced thrombocytopenia necessary?, PMID:39445535
Association of human leucocyte antigen loci with vaccine-induced immune thrombotic thrombocytopenia: Potential role of the interaction between platelet factor 4-derived peptides and MHC-II., PMID:39462764
Efferocytosis dysfunction in CXCL4-induced M4 macrophages: phenotypic insights in systemic sclerosis in vitro and in vivo., PMID:39464886
The dynamic range of immunoassays for heparin-induced thrombocytopenia., PMID:39536820
Prothrombotic antibodies targeting the spike protein's receptor-binding domain in severe COVID-19., PMID:39576992
Absence of platelet overactivation and thrombosis formation among patients with coronary atherosclerosis disease after vaccination against SARS-CoV-2., PMID:39640769
Demystifying autoimmune HIT: what it is, when to test, and how to treat., PMID:39644061
Imaging flow cytometry as a novel approach for the diagnosis of heparin-induced thrombocytopenia., PMID:39658032
Platelet spleen tyrosine kinase is a key regulator of anti-PF4 antibody-induced immunothrombosis., PMID:39705541
Complement activation as a biomarker for platelet-activating antibodies in heparin-induced thrombocytopenia., PMID:39725085
Complex Pattern of Platelet Activation/Reactivity After SARS-CoV-2 Infection., PMID:39795908
Recombinant Anti-PF4 Antibodies Derived from Patients with Vaccine-Induced Immune Thrombocytopenia and Thrombosis (VITT) Facilitate Research and Laboratory Diagnosis of VITT., PMID:39852782
The Platelet-Specific Gene Signature in the Immunoglobulin G4-Related Disease Transcriptome., PMID:39859144
Identification of disulfide bond-linking sites in biosynthesized platelet factor 4 by establishing a partial reduction method without alkylation., PMID:39865791
Anti-platelet Factor 4 Antibody-Mediated Disorders: An Updated Narrative Review., PMID:39884292
Antibodies to PF4 in severe COVID-19: same but different., PMID:39913341
Structural and functional changes underlying activation of monocytes in heparin-induced thrombocytopenia., PMID:39933652
VITT-like Monoclonal Gammopathy of Thrombotic Significance., PMID:39938091
Maternal Anti-PF4 Antibodies as Cause of Neonatal Stroke., PMID:39938100
Guidance-Based Appropriateness of Hemostasis Testing in the Acute Setting., PMID:39970900
Long-term outcomes of heparin-induced thrombocytopenia after cardiac surgery., PMID:40061541
Endothelial cell activation enhances thromboinflammation in vaccine-induced immune thrombotic thrombocytopenia., PMID:40085945
Cooperative effect between anti-PF4/H and anti-PF4 antibodies increases cell activation and thrombotic risk in HIT., PMID:40101232
Lienal Polypeptide Decreases Immune Thrombocytopenia in a Mouse Model by Upregulating Cytokine Production and Increasing the Levels of CD4+, CD8+, and T Regulatory Cells., PMID:40113259
Relevance of anti-platelet factor 4/heparin antibodies and platelet activation in systemic inflammatory diseases and thrombosis disorders: insight from the COVID-19 pandemic., PMID:40123654
Acute Intracerebral Hemorrhage Associated with Extensive Venous Thrombosis Due to Spontaneous Heparin-Induced Thrombocytopenia After Total Knee Replacement: A Case Report., PMID:40126221
Destabilization of PF4-antigenic complexes in heparin-induced thrombocytopenia., PMID:40132149
The Role of CXCL4 in Systemic Sclerosis: DAMP, Auto-Antigen and Biomarker., PMID:40141068
An impetuous "HIT"-sudden blockage of all grafts post CABG., PMID:40144609
Refractory autoimmune heparin-induced thrombocytopenia following cardiac surgery., PMID:40158808
Platelet activating histone/antihistone IgG complexes in anti-PF4 negative thrombosis and thrombocytopenia syndrome., PMID:40179418
Prospective multicenter comparison of the diagnostic value of IgG and IgGAM antibodies for heparin-induced thrombocytopenia., PMID:40187717
PF4-dependent P-selectin expression assay in comparison to the heparin-induced platelet activation assay for the diagnosis of heparin-induced thrombocytopenia., PMID:40191970
Refractory delayed-onset heparin induced thrombocytopenia (HIT) without thrombosis, treated with intravenous immunoglobulin., PMID:40233637
Anti-Platelet factor 4 immunothrombosis-not just heparin and vaccine triggers., PMID:40236285
Soluble P-selectin, but not circulating cell-free DNA, is a potential diagnostic biomarker in heparin-induced thrombocytopenia., PMID:40252846
Case Report: Anti-platelet factor 4 -mediated immunothrombosis in a patient with ANCA vasculitis - a shared mechanism of NETosis., PMID:40276517
PF4-associated immune thrombocytopenia and thrombosis (PITT)-More than heparin and vaccines., PMID:40289696
Anti-PF4 mediated thrombocytopenia and thrombosis associated with acute cytomegalovirus infection displays both HIT-like and VITT-like characteristics., PMID:40298004
Longitudinal assessment of immunogenicity of inactivated COVID-19 booster immunization and breakthrough infection in blood donors: A multicenter study from 2021 to 2023., PMID:40323225
Classification of Platelet-Activating Anti-Platelet Factor 4 Disorders., PMID:40358013
Clinical Analysis of Heparin-Induced Thrombocytopenia due to Therapeutic Plasmapheresis With Heparin Anticoagulation., PMID:40420535
Automated microfluidic electrochemical biosensor for the detection of immune-mediated thrombotic disorders., PMID:40463533
Challenges of Vaccine-Induced Thrombotic Thrombocytopenia-Related Kidney Transplantation: Venous Graft Thrombosis in a Pediatric Recipient., PMID:40482902
For research use only. Not for human or drug use.
