Recombinant Human LAMB3 Protein, N-His

Catalog #: YHG64104 Specific References (0) DATASHEET

Bioactivity for Recombinant Human LAMB3 Protein, N-His ?>

Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Expression system: E. coli

Accession: Q13751

Protein length: Thr587-Lys1172

Overview

Catalog No.

YHG64104

Expression system

E. coli

Species

Homo sapiens (Human)

Protein length

Thr587-Lys1172

Predicted molecular weight

67.24 kDa

Nature

Recombinant

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

Q13751

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 5% Trehalose, 2% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Laminin subunit beta-3, Epiligrin subunit bata, Kalinin B1 chain, Kalinin subunit beta, Laminin B1k chain, Laminin-5 subunit beta, Nicein subunit beta, LAMB3, LAMNB1, laminin-5, laminin-332, epiligrin, kalinin, nicein

Note

For research use only.

Data Image

SDS-PAGE

SDS PAGE for Recombinant Human LAMB3 protein
Bioactivity

LAMB3 regulates collagen matrix stiffness, drives DP cell polarity, and promotes odontoblast differentiation. (A) Schematic diagram showing the culture of DP cells on collagen gels (reconstituted 3 mg/mL collagen I) supplied with 5, 50, or 500 ng/mL LAMB3 protein. (B) Addition of LAMB3 protein primarily increased the stiffness of 3 mg/mL gel. (C) Cytoskeleton distribution visualized using Phalloidin-labeled F-actin staining shows that DP cell morphology changed after culture on collagen gels with the addition of LAMB3. (D) Real-time qPCR shows that the mRNA expression of RHOA was increased by the addition of 50 and 500 ng/mL LAMB3. (E) PCP index of cells cultured on gels supplemented with 5, 50 and 500 ng/mL LAMB3 protein was higher than that of those cultured on 3 mg/mL collagen gel. (F) ABP index of cells cultured on gels supplemented with 50 and 500 ng/mL LAMB3 protein was higher than that of those cultured on 3 mg/mL collagen gel. (G) Real-time qPCR shows that the mRNA expression of PIEZO1, PIEZO2, and ITGB1 increased with increasing concentrations of LAMB3. (H) Real-time qPCR shows that the mRNA expression of DMP1 and DSPP increased with increasing concentrations of LAMB3. (I) Schematic diagram showing the preparation of DM3 germ slice and culture in collagen gels with the addition of LAMB3. (J) HE staining showing the cell morphology in tooth germ slices after a 4-day culture. (K) EdU, TUNEL staining showed cell behavior and immunofluorescence staining was performed to examine the expression of PIEZO1, PIEZO2, ITGB1, and active YAP1; RAC1 and RHOA; DMP1 and DSPP in DM3 germ slice cultured for 4 days in gels supplemented with 5, 50 and 500 ng/mL LAMB3. Scale bar: 50 μm. n = 3 per group. Scale bar: 50 μm. n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = no significance
Data sourced from citation (PMID: DOI: 10.1016/j.cej.2025.164595.)
Bioactivity

LAMB3 regulates collagen gel homogeneity and fibril connectivity. (A) Representative image of collagen matrix gels reconstituted with increasing concentrations. (B, C) Representative images of collagen matrix gels reconstituted with gradient concentrations of LAMB3 or FN. (D) Quantification analysis of topological characterization of reconstituted collagen matrix gels with increasing concentrations. (E, F) Quantification analysis of topological characterization of reconstituted collagen matrix gels with gradient concentrations of LAMB3 or FN. Scale bar: 50 μm. n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001, ns = no significance.
Data sourced from citation (PMID: DOI: 10.1016/j.cej.2025.164595.)

References

Datasheet

Document Download

Recombinant Human LAMB3 Protein, N-His.pdf

$ 313

Product specifications

100 μg 313 1 mg 1629

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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