InVivoMAb Anti-Human IL8/CXCL8 (Iv0023)

Catalog #: VHC79101 Specific References (0) DATASHEET

Flow-cytometry for InVivoMAb Anti-Human IL8/CXCL8 (Iv0023) ?>

Bioactivity for InVivoMAb Anti-Human IL8/CXCL8 (Iv0023) ?>

Host species: Mouse

Isotype: IgG1, kappa

Applications: ELISA, FCM, Neutralization

Accession: P10145

Overview

Catalog No.

VHC79101

Species reactivity

Human

Host species

Mouse

Isotype

IgG1, kappa

Clonality

Monoclonal

Applications

ELISA, FCM, Neutralization

Target

GCP/IL-8 protein II, MDNCF, IL8/NAP1 form III, IL-8(1-77), Neutrophil-activating factor, GCP/IL-8 protein I, GCP/IL-8 protein VI, Interleukin-8, Chemokine (C-X-C motif) ligand 8, Neutrophil-activating protein 1, Monocyte-derived neutrophil chemotactic factor, (Ser-IL-8)72, MDNCF-b, LYNAP, GCP/IL-8 protein V, IL8/NAP1 form IV, MONAP, IL8/NAP1 form V, IL8/NAP1 form VI, NAP-1, Granulocyte chemotactic protein 1, GCP/IL-8 protein III, IL8/NAP1 form II, IL8, IL-8, CXCL8, IL8/NAP1 form I, (Ala-IL-8)77, Protein 3-10C, GCP/IL-8 protein IV, Emoctakin, GCP-1, C-X-C motif chemokine 8, NAF, T-cell chemotactic factor, MDNCF-c, Monocyte-derived neutrophil-activating peptide, Lymphocyte-derived neutrophil-activating factor

Concentration

2.22 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P10145

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Iv0023

Note

For research use only. Not suitable for clinical or therapeutic use.

Data Image

Western Blot

Various lysates were subjected to SDS PAGE followed by western blot with Anti Human IL8/CXCL8 antibody [Iv0023] (VHC79101) at 1μg/ml.

Lane 1: Human IL8/CXCL8 transfected HEK293 cell lysate
Lane 2: Non-transfected HEK293 cell lysate

Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

Predict MW: 12 kDa
Flow-cytometry

Flow-cytometry using anti-human IL8 antibody.IL8 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL8 antibody monoclonal antibody (Catalog # VHC79101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
Western Blot

Fig.4 IL-1β induces the expression of DDX21 in CRC via JAK2-STAT3 signaling pathway. (A) p-JAK2, p-STAT3, p-ERK, β-catenin expression in CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells by Western blot analysis. (B) The size and number of spheres of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (C-D) The number of colony formation (C), migration and invasion ability (D) of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (E) Expression of p-JAK2, pSTAT3, p-ERK, β-catenin, stemness markers, and EMA-related markers in CRC cells (HCT116 and HT29) si-NC, si-DDX21, IL-1β-supplemented si-NC, IL-1β-supplemented si-DDX21 CRC cells by Western blot analysis
Data sourced from citation (PMID: 41160952)
Bioactivity

Fig.4 IL-1β induces the expression of DDX21 in CRC via JAK2-STAT3 signaling pathway. (A) p-JAK2, p-STAT3, p-ERK, β-catenin expression in CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells by Western blot analysis. (B) The size and number of spheres of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (C-D) The number of colony formation (C), migration and invasion ability (D) of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (E) Expression of p-JAK2, pSTAT3, p-ERK, β-catenin, stemness markers, and EMA-related markers in CRC cells (HCT116 and HT29) si-NC, si-DDX21, IL-1β-supplemented si-NC, IL-1β-supplemented si-DDX21 CRC cells by Western blot analysis
Data sourced from citation (PMID: 41160952)

References

Datasheet

Document Download

InVivoMAb Anti-Human IL8/CXCL8 (Iv0023).pdf

$ 268

Product specifications

1 mg 268 5 mg 880 25 mg 2800

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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