InVivoMAb Anti-Human TNFa/TNF-alpha (Iv0050)

Catalog #: VHB94401 Specific References (0) DATASHEET

SEC-HPLC for InVivoMAb Anti-Human TNFa/TNF-alpha (Iv0050) ?>

Western Blot for InVivoMAb Anti-Human TNFa/TNF-alpha (Iv0050) ?>

Bioactivity for InVivoMAb Anti-Human TNFa/TNF-alpha (Iv0050) ?>

Host species: Human

Isotype: IgG1, kappa

Applications: ELISA, FCM, Neutralization

Accession: P01375

Overview

Catalog No.

VHB94401

Species reactivity

Human

Host species

Human

Isotype

IgG1, kappa

Clonality

Monoclonal

Applications

ELISA, FCM, Neutralization

Target

TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1

Concentration

2.97 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01375

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Iv0050

Note

For research use only. Not suitable for clinical or therapeutic use.

Data Image

Flow-cytometry

Flow-cytometry using anti-human TNFa antibody.TNFa Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # VHB94401 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
SEC-HPLC

The purity of this product is >95% as determined by SEC-HPLC.
Western Blot

Fig.4 IL-1β induces the expression of DDX21 in CRC via JAK2-STAT3 signaling pathway. (A) p-JAK2, p-STAT3, p-ERK, β-catenin expression in CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells by Western blot analysis. (B) The size and number of spheres of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (C-D) The number of colony formation (C), migration and invasion ability (D) of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (E) Expression of p-JAK2, pSTAT3, p-ERK, β-catenin, stemness markers, and EMA-related markers in CRC cells (HCT116 and HT29) si-NC, si-DDX21, IL-1β-supplemented si-NC, IL-1β-supplemented si-DDX21 CRC cells by Western blot analysis
Data sourced from citation (PMID: 41160952)
Bioactivity

Fig.4 IL-1β induces the expression of DDX21 in CRC via JAK2-STAT3 signaling pathway. (A) p-JAK2, p-STAT3, p-ERK, β-catenin expression in CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells by Western blot analysis. (B) The size and number of spheres of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (C-D) The number of colony formation (C), migration and invasion ability (D) of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (E) Expression of p-JAK2, pSTAT3, p-ERK, β-catenin, stemness markers, and EMA-related markers in CRC cells (HCT116 and HT29) si-NC, si-DDX21, IL-1β-supplemented si-NC, IL-1β-supplemented si-DDX21 CRC cells by Western blot analysis
Data sourced from citation (PMID: 41160952)

References

Datasheet

Document Download

InVivoMAb Anti-Human TNFa/TNF-alpha (Iv0050).pdf

$ 268

Product specifications

1 mg 268 5 mg 880 25 mg 2800

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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