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Mouse Anti-Monkeypox virus/MPXV E8L Antibody (SAA0285)

Catalog #:   RVV13201 Specific References (0) DATASHEET
Host species: Mouse
Isotype: IgG2a, kappa
Applications: ELISA, Neutralization, WB
Accession: NP_536532.1
Overview

Catalog No.

RVV13201

Species reactivity

Monkeypox virus(MPXV)

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Applications

ELISA, Neutralization, WB

Target

E8L, Cell surface-binding protein, Carbonic anhydrase homolog

Concentration

2.56 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

NP_536532.1

Form

Liquid

Storage buffer

100 mM Pro-Ac, 20 mM Arg, pH 5.0

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0285

Note

For research use only.

Data Image
  • Western Blot
    Recombinant Protein were subjected to SDS PAGE followed by western blot with Monkeypox virus/MPXV E8L antibody (RVV13201) at 1ug/ml.

    Lane 1: Recombinant Monkeypox virus/MPXV E8L Protein(EVV13201)

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 40 kDa
    Observed MW: 40 kDa
  • Western Blot
    Recombinant Protein were subjected to SDS PAGE followed by western blot with Monkeypox virus/MPXV E8L antibody (RVV13201) at 1ug/ml.

    Lane 1: Recombinant Monkeypox virus/MPXV E8L Protein(YVV13201)

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 33 kDa
    Observed MW: 33 kDa
  • Bioactivity
    Detects Monkeypox virus/MPXV E8L in indirect ELISAs.
  • SDS-PAGE
    SDS PAGE for Anti-MPXV E8L Antibody (SAA0285)
  • Immunofluorescence
    (B) MPXV protein expression in HEK293T cells. Cells were transfected with antigen-encoded mRNAs and detected at 24 h post transfection by indirect immunofluorescence staining. Scar bar, 50 μm.
    Data sourced from citation (PMID: 36947428)
  • Western Blot
    Expression and characterization of recombinant MPXV proteins A29, E8, M1, A35, and B6 using SDS-PAGE (left) and Western blot (right).
    Data sourced from citation (PMID: 39772071)
  • Western Blot
    MPXV-F3L/E8L protein expression analyzed by Western blot.
    Data sourced from citation (PMID: 40651557)
  • Western Blot
    FIGURE 1 | Establishment of an MPXV infection model in human Sertoli cells (F) Western blot analysis is performed to detect MPXV E8L, MPXVF3L, and the internal control protein Tubulin in Sertoli cells.
    Data sourced from citation (PMID: 41181874)
References
Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

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Distributor list

For research use only. Not for human or drug use.

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Mouse Anti-Monkeypox virus/MPXV E8L Antibody (SAA0285) [RVV13201]
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