Anti-ACTB/β-actin/Beta Actin Antibody (SAA0339)

Catalog #: RHF37501 Specific References (0) DATASHEET

Host species: Mouse

Isotype: IgG2a, kappa

Applications: ELISA, WB

Accession: P60709

Overview

Catalog No.

RHF37501

Description

Loading control in ELISA,WB

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Applications

ELISA, WB

Recommended Dilution

Application	Dilution
ELISA	1:2000-1:4000, WB: 1:1000-1:4000

Target

Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB

Concentration

0.95 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P60709

Form

Liquid

Storage buffer

315mM Arginine, pH 8.0, 173mM H3PO4, 0.043% Tween 20

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0339

Note

For research use only.

Data Image

Western blot

Various lysates were subjected to SDS PAGE followed by western blot with ACTB/β-actin/Beta Actin antibody (RHF37501) at 0.2 μg/ml.

Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: PC12 cell lysate
Lane 5: BV2 cell lysate

Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

Predict MW: 42 kDa
Observed MW: 42 kDa
Western blot

Various lysates were subjected to SDS PAGE followed by western blot with ACTB/β-actin/Beta Actin antibody (RHF37501) at 0.2 μg/ml.

Lane 1: mouse kidney lysate
Lane 2: mouse lung lysate
Lane 3: rat kidney lysate
Lane 4: rat lung lysate

Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

Predict MW: 42 kDa
Observed MW: 42 kDa
Western Blot

Fig. 4. TRAF2 antibody specificity analysis by WB. A: WB results of TRAF2 in ducks incubated by chicken TRAF2 antibody. B: WB results of TRAF2 in mouse incubated by chicken TRAF2 antibody. C: WB results of TRAF2 in rabbit incubated by chicken TRAF2 antibody. D: WB results of TRAF2 in goat incubated by chicken TRAF2 antibody.
Data sourced from citation (PMID: 39798767)

References

Datasheet

Document Download

Anti-ACTB/β-actin/Beta Actin Antibody (SAA0339).pdf

$ 130

Product specifications

50 μg 130 100 μg 220

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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