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Anti-Monkeypox virus/MPXV E8L Polyclonal Antibody

Catalog #:   PVV13201 Specific References (0) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, IF, WB
Overview

Catalog No.

PVV13201

Species reactivity

Monkeypox virus (strain Zaire-96-I-16) (MPX)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Monkeypox virus/MPXV E8L (Met1-Lys274).

Applications

ELISA, IHC, IF, WB

Recommended Dilution

Application Dilution
ELISA 1:5000-1:20000, IHC: 1:50-1:500, IF: 1:50-1:500, WB: 1:500-1:2000

Target

E8L, Cell surface-binding protein, Carbonic anhydrase homolog

Concentration

0.76 mg/ml

Purification

Purified by antigen affinity column.

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • Western Blot
    Recombinant Protein were subjected to SDS PAGE followed by western blot with Monkeypox virus/MPXV E8L antibody (PVV13201) at 1ug/ml.

    Lane 1: Recombinant Monkeypox virus/MPXV E8L Protein(EVV13201)

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 40 kDa
    Observed MW: 40 kDa
  • Western Blot
    Recombinant Protein were subjected to SDS PAGE followed by western blot with Monkeypox virus/MPXV E8L antibody (PVV13201) at 1ug/ml.

    Lane 1: Recombinant Monkeypox virus/MPXV E8L Protein(YVV13201)

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 33 kDa
    Observed MW: 33 kDa
  • Western Blot
    Figure 5. Targeting MYST1 or aerobic glycolysis inhibits MPXV replication D) Cells treated as in (C) were used to measure the levels of MPXV proteins (F3L and E8L) H) Cells treated as in (G) were used to measure the protein levels of MPXV genes (F3L and E8L). L) Cells treated as in (K) were used to measure the protein levels of MPXV genes (F3L and E8L)
    Data sourced from citation (PMID: 41144710)
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Monkeypox virus/MPXV E8L Polyclonal Antibody [PVV13201]
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