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Anti-Monkeypox virus/MPXV A35R Polyclonal Antibody

Catalog #:   PVV13101 Specific References (0) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, IF, WB
Overview

Catalog No.

PVV13101

Species reactivity

Monkeypox virus (MPXV)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Monkeypox virus/MPXV A35R (Val57-Thr181).

Applications

ELISA, IHC, IF, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:200, IF: 1:50-1:100, WB: 1:1000-1:4000

Target

A35R, Bifunctional EEV membrane phosphoglycoprotein, Bifunctional EEV membrane phosphoglycoprotein/associates with A36R, Bifunctional EEV membrane protein, EEV membrane phosphoglycoprotein, EEV membrane phosphoglycoprotein C-type,EEV membrane phosphoglycoprotein, C-type lectin-like domain, MPXV-COP-139, MPXV-SL-139, MPXV-WRAIR139

Concentration

2.36 mg/ml

Purification

Purified by antigen affinity column.

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • Western Blot
    Recombinant Protein were subjected to SDS PAGE followed by western blot with Monkeypox virus/MPXV A35R antibody (PVV13101) at 1ug/ml.

    Lane 1: Recombinant Monkeypox virus/MPXV A35R Protein(EVV13101)

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 16 kDa
    Observed MW: 16 kDa
  • Western Blot
    Recombinant Protein were subjected to SDS PAGE followed by western blot with Monkeypox virus/MPXV A35R antibody (PVV13101) at 1ug/ml.

    Lane 1: Recombinant Monkeypox virus/MPXV A35R Protein(YVV13101)

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 16 kDa
    Observed MW: 16 kDa
  • Flow Cytometry
    (F and G) Flow cytometry analysis confirming the protein expression of MPXV mRNA in HEK-293T cells.
    Data sourced from citation (PMID: 38787725)
  • Western Blot
    The four circRNAs were transfected into 293T cells, and the expression of each protein was determined by western blot.
    Data sourced from citation (PMID: 38659224)
  • Western Blot
    Fig. 1 | Design, construction, and validation of MPXV mRNA vaccines. G HeLa cells were transfected with 2 μg of mRNA, and cell lysates collected at 24 h posttransfection were analyzed by SDS-PAGE and Western blotting with anti-A35R and anti-β-actin antibodies.
    Data sourced from citation (PMID: 40783493)
  • Immunofluorescence
    Validation of MPXV mRNA protein expression in HEK-293T cells by immunofluorescence. Scale bar, 150 mm.
    Data sourced from citation (PMID: 38787725)
  • Immunofluorescence
    The four circRNAs were transfected into 293T cells, and the expression of each protein was determined by immunofluorescence analysis (IFA).
    Data sourced from citation (PMID: 38659224)
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Monkeypox virus/MPXV A35R Polyclonal Antibody [PVV13101]
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