Buy Anti-Nipah virus/HeV G protein/Glycoprotein G Polyclonal Antibody (Rabbit, IgG)

Anti-Nipah virus/HeV G protein/Glycoprotein G Polyclonal Antibody

Catalog #: PVV07901 Specific References (0) DATASHEET

Host species: Rabbit

Isotype: IgG

Applications: ELISA, IHC, WB

Overview

Catalog No.

PVV07901

Species reactivity

Nipah virus(HeV)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Nipah virus/HeV G protein/Glycoprotein G1 (Gln71-Thr602).

Applications

ELISA, IHC, WB

Recommended Dilution

Application	Dilution
ELISA	1:4000-1:8000, IHC:1:50-1:200, WB: 1:1000-1:4000

Target

Glycoprotein G, G

Concentration

1.35 mg/ml

Purification

Purified by antigen affinity column.

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image

WESTERN BLOT

Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with G protein/Glycoprotein G antibody (PVV07901) at 1 μg/ml.

Lane 1: Recombinant protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 62 kDa
Observed MW: 62 kDa
Western Blot

All plasmids were transfected into 293 T cells and MVA-T7-infected 293 T cells. After 48 h, the RLuc expression was detected by immunofluorescence assay (C), and expressions of F and G were detected by western blotting assay (D)
Data sourced from citation (PMID: 38865205)
Western Blot

(C) Cell lysates were collected 24 h p.i. and subjected to western blot analysis. NiV N, F, G, and M were stained with respective specific antibodies, biotinylated secondary antibodies, and HRP-conjugated streptavidin. Chemiluminescent signals were recorded with a Chemidoc system.
Data sourced from citation (PMID: 41378893)
Western Blot

(c)Western blot analysis of capture chromatography step. NiV G protein is detected in E2-E5 elution fractions. SN: cell culture supernatant; FT: chromatography flow-through; E1-5: elution fractions.
Data sourced from citation (PMID: 41476213)
Western Blot

(c) NiVLP G and BV gp64 detection after CEX with western blot analysis. The majority of NiV G is detected in E2 fraction, which was further purified on AEX. A280, A260 and conductivity readings are blue, red and dashed orange lines, respectively.
Data sourced from citation (PMID: 41476213)
Western Blot

(f) NiV G and gp64 detection with western blot after AEX. The majority of NiV G-containing NiVLPs are in E2 fraction, which overlaps with BV elution. Fractions E1 and E2 were pooled and sterile filtered for immunogenicity study. FT: flow-through; E1-4: elution fractions; rG: recombinant NiV G.
Data sourced from citation (PMID: 41476213)

References

Datasheet

Document Download

Anti-Nipah virus/HeV G protein/Glycoprotein G Polyclonal Antibody.pdf

$ 170

Product specifications

50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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