Anti-Human TNNT2 Polyclonal Antibody

Catalog #: PHE44901 Specific References (0) DATASHEET

Bioactivity for Anti-Human TNNT2 Polyclonal Antibody ?>

Host species: Rabbit

Isotype: IgG

Applications: ELISA, IHC, WB

Accession: P45379

Overview

Catalog No.

PHE44901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TNNT2 (Phe97-Lys298).

Applications

ELISA, IHC, WB

Recommended Dilution

Application	Dilution
ELISA	1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Troponin T, cardiac muscle, TnTc, cTnT, TNNT2, Cardiac muscle troponin T

Concentration

1.01 mg/ml

Purification

Purified by antigen affinity column.

Accession

P45379

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image

WESTERN-BLOT

Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with TNNT2 antibody (PHE44901) at 1 μg/ml.

Lane 1: Recombinant protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 27 kDa
Observed MW: 27 kDa
Western Blot

Various lysates were subjected to SDS PAGE followed by western blot with TNNT2 antibody (PHE44901) at 1 µg/ml.

Lane 1: Mouse heart lysate
Lane 2: Rat heart lysate

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 36 kDa
Observed MW: 40 kDa
Bioactivity

Figure 6. Profiling of six cardiac biomarkers in clinical samples. (a) Acquired Raman signal distribution for all clinical samples, where the x-axis represents the disease groups and the y-axis depicts the normalized Raman intensity of each sample, calculated by dividing the detected intensities by those of the corresponding SERS barcodes. (b) Raman spectra and (c) heatmap from several representative samples of each sample, calculated by dividing the detected intensities by those of the corresponding SERS barcodes and a subsequent min−max scaling. LDA for the classification of (d) CRD and CAD and (e) CRD, CAD, and MI with the normalized Raman intensities of clinical samples. (f) ROC curve for the LDA model in classifying CAD versus CRD, evaluated using stratified 5-fold cross-validation. (g) Comparison of ELISA and Raman for analyzing the same clinical samples. The x-axis represents the clinical samples and the y-axis shows the concentration (ng/mL).
Data sourced from citation (PMID: 40580100)

References

Datasheet

Document Download

Anti-Human TNNT2 Polyclonal Antibody.pdf

$ 170

Product specifications

50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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