Anti-MPXV A35R Human IgG ELISA Kit

Catalog #: KAV13102 Specific References (0) DATASHEET

ELISA for Anti-MPXV A35R Human IgG ELISA Kit ?>

Applications: Used for the quantitative determination of Human Anti-MPXV A35R IgG concentration in serum and plasma.

Sample type: Plasma, Serum

Sensitivity: 0.62 ng/mL

Range: 0.63 - 20 ng/mL

Overview

Catalog No.

KAV13102

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant MPXV A35R antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human Anti-MPXV A35R IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human Anti-MPXV A35R IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Human Anti-MPXV A35R IgG concentration in serum and plasma.

Target

A35R

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

0.63 - 20 ng/mL

Sensitivity

0.62 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	9.6	2.5	1.3	11.3	2.9	0.8
Standard deviation	0.4	0.1	0.0	0.4	0.2	0.1
CV (%)	4.0	2.4	2.8	3.6	5.5	10.4

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.

Note

For Research Use Only.

Data Image

Experiment Example

CALCULATION OF RESULTS
Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Anti-MPXV A35R Human IgG concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
ELISA

Kinetics of MPXV-specific IgG responses. (A-B) levels of anti-A29L, E8L, and A35R IgG antibody in PWoH (n = 7 at 1–2 weeks; n = 3 at 1 month; n = 2 at 3 months; n = 2 at 6 months; n = 3 at 12 months; n = 5 at 18 months) and PWH (n = 7, 10, 3, 5, 7, and 5 at corresponding time points). Bar plots represented group-wise comparisons at each time point; line graphs illustrated temporal dynamics. Data were shown as geometric means ± geometric standard deviation. Statistical comparisons used the mann-whitney U test; P values were indicated when significant. Abbreviations: w, weeks; m, months. (C) dynamic changes of anti-A29L, E8L, and A35R IgG antibodies in each participant after the onset of symptoms (PWoH: n = 13, samples = 26; PWH: n = 28, samples = 52)
Data sourced from citation (PMID: 42216225)

References

Datasheet

Document Download

Anti-MPXV A35R Human IgG ELISA Kit.pdf

$ 1128

Product specifications

96 T 1128

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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