Affiliations
- Wuhan Institute of Biological Products Co., Ltd., Wuhan, People's Republic of China.
- China National Biotec Group (CNBG), Beijing, People's Republic of China.
PMID: 37128898 PMCID: PMC10177700 DOI: 10.1080/22221751.2023.2208682
Abstract
Background: Since May 2022, human mpox cases have increased unexpectedly in non-endemic countries. The first imported case of human mpox in Hong Kong was reported in September 2022. This study reports the isolation and identification of the Mpox virus (MPXV) from this patient.
Methods: Vesicle swabs were inoculated in Vero and Vero E6 cells. Cytopathic effects (CPEs) were observed, and the virus was identified using quantitative RT-PCR, electron microscopy, and high-throughput sequencing.
Results: CPEs were visible at 24 h and 48 h in Vero E6 and Vero cells, respectively. The MPXV strain belongs to the B.1 branch of the West African lineage. Neutralizing antibody tests showed limited cross-protection in sera from smallpox-vaccinated individuals.
Conclusion: MPXV strains were successfully isolated and identified, providing insights for therapeutic development and disease control.
Keywords
Isolation; Mpox virus; identification; neutralizing antibodies; phylogenetic.
