Affiliations
- College of Biology, Hunan University, Changsha, 410082, China.
- State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
- State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. jianhuijiang@hnu.edu.cn.
- College of Biology, Hunan University, Changsha, 410082, China. jianjunh@hnu.edu.cn.
PMID: 33744669 DOI: 10.1016/j.bios.2021.113133
Abstract
Background: Although suspension array technology (SAT) using encoded microspheres provides high-quality results for bioanalytic applications, current encoding strategies limit distinguishable codes. This study introduces density-encoded hydrogel microcarriers (DMs) using the density property of biomaterials as a high-capacity coding dimension.
Results: DMs are synthesized using different monomer ratios, enabling simultaneous decoding and separation with density gradient centrifugation. They are compatible with flow cytometry detection, and an 8 × 2 × 4 (density × size × color) library was constructed. Aptamer-functionalized DMs (ADMs) demonstrated effective 4-plex protein quantification with sub-nanomolar detection limits and were useful for label-free detection of tumor-derived exosomes.
Conclusion: The simplicity and functionality of DMs can advance suspension arrays and inspire the development of DM-based diagnostic applications.
Keywords：Density-encoded; Hydrogel; Microcarrier; Multiplexed bioassays.
