Catalog No.
YXX07001
Expression system
E. coli
Species
Treponema pallidum (strain Nichols)
Protein length
Cys20-Gln434
Predicted molecular weight
47.48 kDa
Nature
Recombinant
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P29723
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Putative DD-carboxypeptidase TP_0574, 3.4.-.-, 47 kDa lipoprotein, Tp47, Tpp47, 47 kDa membrane antigen, 47-kilodalton major integral membrane immunogen, TP_0574
Note
For research use only.
The 47-kDa major lipoprotein immunogen of Treponema pallidum is a penicillin-binding protein with carboxypeptidase activity., PMID:7972112
Comparison of molecular and microscopic techniques for detection of Treponema pallidum in genital ulcers., PMID:7535311
Membrane topology of Borrelia burgdorferi and Treponema pallidum lipoproteins., PMID:7790053
Molecular mimicry between an immunodominant amino acid motif on the 47-kDa lipoprotein of Treponema pallidum (Tpp47) and multiple repeats of analogous sequences in fibronectin., PMID:8752922
Activation of human monocytic cells by Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides proceeds via a pathway distinct from that of lipopolysaccharide but involves the transcriptional activator NF-kappa B., PMID:8751937
T-Cell responses to Treponema pallidum subsp. pallidum antigens during the course of experimental syphilis infection., PMID:10456928
The cutaneous response in humans to Treponema pallidum lipoprotein analogues involves cellular elements of both innate and adaptive immunity., PMID:11238663
Toll-like receptor 2 ligands as adjuvants for human Th1 responses., PMID:12496400
Detection of the 47-kilodalton membrane immunogen gene of Treponema pallidum in various tissue sources of patients with syphilis., PMID:15698722
Preparation of a visual protein chip for detection of IgG against Treponema pallidum., PMID:16170212
Diagnosing Treponema pallidum in secondary syphilis by PCR and immunohistochemistry., PMID:17554371
[Optimization of microbial synthesis of recombinant analogs of Treponema pallidum membrane proteins (Tpp17 and Tpp47) and estimation of their immunochemical properties]., PMID:17682528
Profile of anti-Tp47 antibodies in patients with positive serology for syphilis analized by Western Blot., PMID:18641851
Macrolide resistance and molecular types of Treponema pallidum causing primary syphilis in Shanghai, China., PMID:19583516
Getting the measure of syphilis: qPCR to better understand early infection., PMID:21752804
Evaluation of a PCR test for detection of treponema pallidum in swabs and blood., PMID:22219306
Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers., PMID:25520453
Comparison of Diagnostic Accuracy of PCR Targeting the 47-Kilodalton Protein Membrane Gene of Treponema pallidum and PCR Targeting the DNA Polymerase I Gene: Systematic Review and Meta-analysis., PMID:26311859
Editorial Commentary: Polymerase Chain Reaction for the tpp47 Gene: A New Test for Neurosyphilis., PMID:27585980
Sensitivity of nPCR for four types of membrane protein DNA and of two pairs of primers for Tpp47 DNA of Treponema pallidum in whole blood of congenital syphilis newborns., PMID:28889773
A lung abscess caused by secondary syphilis - the utility of polymerase chain reaction techniques in transbronchial biopsy: a case report., PMID:31288744
Analysis of DNA extraction methods for detection of Treponema pallidum: A comparison of three methods., PMID:34826521
A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples., PMID:35945210
For research use only. Not for human or drug use.
