Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (A35R) (KAV13101)

Catalog No.

KAV13101

Description

PRINCIPLE OF THE ASSAY. This assay employs the quantitative competitive enzyme immunoassay technique. A recombinant Monkeypox virus A35R antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox Virus IgG (A35R) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox Virus IgG (A35R). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox Virus IgG (A35R) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

A35R

Applications

Used for the quantitative determination of Human Anti-Monkeypox Virus IgG (A35R) concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

312.5 ng/mL - 20000 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <20%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <20%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	10326.5	3001.4	736.1	9833.3	2814.0	737.1
Standard deviation	805.2	419.3	138.3	797.2	282.6	117.7
CV (%)	7.8	14.0	18.8	8.1	10.0	16.0

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.

Characterization of the vaccinia virus A35R protein and its role in virulence. PMID: 16352555

Enhancing the Immunogenicity of Vaccinia Virus. PMID: 35891430

Vaccinia virus A35R inhibits MHC class II antigen presentation. PMID: 19954808

Monkeypox infection elicits strong antibody and B cell response against A35R and H3L antigens. PMID: 36687315

Mpox vaccine and infection-driven human immune signatures. PMID: 36945651

Experimental Evolution Identifies Vaccinia Virus Mutations in A24R and A35R That Antagonize the Protein Kinase R Pathway and Accompany Collapse of an Extragenic Gene Amplification. PMID: 26202237

Rational development of multicomponent mRNA vaccine candidates against mpox. PMID: 36947428

Enhancing the Protective Immune Response to Administration of a LIVP-GFP Live Attenuated Vaccinia Virus to Mice. PMID: 33801026

Development of multi-epitope vaccines against the monkeypox virus based on envelope proteins using immunoinformatics approaches. PMID: 36993967

Three neutralizing mAbs induced by MPXV A29L protein recognizing different epitopes act synergistically against orthopoxvirus. PMID: 37288876

Heterogeneity in the A33 protein impacts the cross-protective efficacy of a candidate smallpox DNA vaccine. PMID: 18482742

Highly immunogenic variant of attenuated vaccinia virus. PMID: 27025484

Monkeypox virus quadrivalent mRNA vaccine induces immune response and protects against vaccinia virus. PMID: 37117161

Design peptide and multi-epitope protein vaccine candidates against monkeypox virus using reverse vaccinology approach: an in-silico study. PMID: 37154825

Vaccinia virus egress mediated by virus protein A36 is reliant on the F12 protein. PMID: 28631604

Deletion of the A35 gene from Modified Vaccinia Virus Ankara increases immunogenicity and isotype switching. PMID: 21352940

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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (A35R).pdf