T5 Exonuclease

Catalog No.

YVV16101

Expression system

E.coli

Species

Escherichia phage T5 (Enterobacteria phage T5)

Protein length

T5 Exonuclease is cloned from Escherichia phage T5 and expressed in E.coli.

Predicted molecular weight

35.61 kDa

Nature

Recombinant

Target

T5 Exonuclease

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Form

Liquid

Storage buffer

50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.5 @ 25℃

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Alternative Names

T5 Exonuclease

T5 Exonuclease Improves the Identification of Aptamers Specific for Small Molecules from the Early Screening Library., PMID:40222044

Selection of novel aptamers specific for tenuazonic acid and their aptasensors., PMID:40132410

Base-pair resolution reveals clustered R-loops and DNA damage-susceptible R-loops., PMID:40112807

DAPE cloning with modified primers for producing designated lengths of 3' single-stranded ends in PCR products., PMID:39946422

Synthesizing unmodified, supercoiled circular DNA molecules in vitro., PMID:39896529

Novel Nuclease MbovP701 with a Yqaj Domain Is Interrelated with the Growth of Mycoplasma bovis., PMID:39770711

Engineering a robust Cas12i3 variant-mediated wheat genome editing system., PMID:39690508

HPV16 genome structure analysis in oropharyngeal cancer PDXs identifies tumors with integrated and episomal genomes., PMID:38936774

In Vivo DNA Assembly Using the PEDA Method., PMID:38468102

A Fluorescence-Based, T5 Exonuclease-Amplified DNA Cleavage Assay for Discovering Bacterial DNA Gyrase Poisons., PMID:37904923

TLTC, a T5 exonuclease-mediated low-temperature DNA cloning method., PMID:37635997

Development of miniature base editors using engineered IscB nickase., PMID:37231266

The role of size in biostability of DNA tetrahedra., PMID:37021956

Addition of the T5 exonuclease increases the prime editing efficiency in plants., PMID:36958601

The role of size in biostability of DNA tetrahedra., PMID:36945546

Glyphosate-Exonuclease Interactions: Reduced Enzymatic Activity as a Route to Glyphosate Biosensing., PMID:36808212

Quantification of the hepatitis B virus cccDNA: evidence-based guidelines for monitoring the key obstacle of HBV cure., PMID:36707234

Hypericin blocks the function of HSV-1 alkaline nuclease and suppresses viral replication., PMID:35811028

Site-Directed Mutagenesis Method Mediated by Cas9., PMID:35727450

ErCas12a and T5exo-ErCas12a Mediate Simple and Efficient Genome Editing in Zebrafish., PMID:35336785

Phage Enzyme-Assisted Direct In Vivo DNA Assembly in Multiple Microorganisms., PMID:35298132

Untying relaxed circular DNA of hepatitis B virus by polymerase reaction provides a new option for accurate quantification and visualization of covalently closed circular DNA., PMID:35130138

Quantification of intrahepatic cccDNA in HBV associated hepatocellular carcinoma by improved ddPCR method., PMID:34688781

A T5 Exonuclease-Based Assay for DNA Topoisomerases and DNA Intercalators., PMID:34056374

5'-DMT-protected double-stranded DNA: Synthesis and competence to enzymatic reactions., PMID:33508272

CT5, a subtle in vitro DNA assembling method based on the combination of FnCas12a and T5 exonuclease., PMID:33389273

Improving FnCas12a Genome Editing by Exonuclease Fusion., PMID:33346706

Conformation and protein interactions of intramolecular DNA and phosphorothioate four-way junctions., PMID:33342281

Label-free profiling of DNA aptamer-small molecule binding using T5 exonuclease., PMID:33053182

Visual detection of Fusarium proliferatum based on dual-cycle signal amplification and T5 exonuclease., PMID:35515665

Fusing T5 exonuclease with Cas9 and Cas12a increases the frequency and size of deletion at target sites., PMID:32382982

A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method., PMID:32152687

Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning., PMID:32010678

Seamless insert-plasmid assembly at sub-terminal homologous sequences., PMID:31669339

Quantification of Hepatitis B Virus Covalently Closed Circular DNA in Infected Cell Culture Models by Quantitative PCR., PMID:33654998

T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis., PMID:30462336

Oligo swapping method for in vitro DNA repair substrate containing a single DNA lesion at a specific site., PMID:30459925

T5 Exonuclease Hydrolysis of Hepatitis B Virus Replicative Intermediates Allows Reliable Quantification and Fast Drug Efficacy Testing of Covalently Closed Circular DNA by PCR., PMID:30232183

Rapid and Error-Free Site-Directed Mutagenesis by a PCR-Free In Vitro CRISPR/Cas9-Mediated Mutagenic System., PMID:30075075

Crystal structure and mutational analysis of Mycobacterium smegmatis FenA highlight active site amino acids and three metal ions essential for flap endonuclease and 5' exonuclease activities., PMID:29635474

Direct observation of DNA threading in flap endonuclease complexes., PMID:27273516

Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost., PMID:27073895

Generation of porcine reproductive and respiratory syndrome virus by in vitro assembly of viral genomic cDNA fragments., PMID:25300804

Ultrasensitive detection of 3'-5' exonuclease enzymatic activity using molecular beacons., PMID:24404561

Neutralizing mutations of carboxylates that bind metal 2 in T5 flap endonuclease result in an enzyme that still requires two metal ions., PMID:21734257

Enzymatic assembly of overlapping DNA fragments., PMID:21601685

Brønsted analysis and rate-limiting steps for the T5 flap endonuclease catalyzed hydrolysis of exonucleolytic substrates., PMID:20698567

Substrate recognition and catalysis by flap endonucleases and related enzymes., PMID:20298197

Three metal ions participate in the reaction catalyzed by T5 flap endonuclease., PMID:18697748

Comparison of the catalytic parameters and reaction specificities of a phage and an archaeal flap endonuclease., PMID:17559871

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