Catalog No.
YXX06401
Biological activity
37 ℃, pH 8.0 reaction conditions, 2.625 mL reaction system, change the absorption value of △ A260 to 1.0 in 30 minutes（equivalent to the complete digestion of 37 μg salmon sperm DNA into an oligonucleotide). The amount of enzyme used is defined as an activity Unit（U)
Expression system
E.coli
Species
Serratia marcescens
Protein length
Benzonase Nuclease is cloned from Serratia marcescens and expressed in E.coli.
Predicted molecular weight
30.16 kDa
Nature
Recombinant
Target
Benzonase Nuclease/Endonuclease
Concentration
200U/μL
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Liquid
Storage buffer
20mM Tris-HCl, pH8.0, 2mM MgCl2, 20mM NaCl, 50% Glycerol.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Alternative Names
Benzonase Nuclease, Endonuclease
Note
For research use only.
Quantitative determination of the infectivity of the proviral DNA of a retrovirus in vitro: Evaluation of methods for DNA inactivation., PMID:19457682
Post-ischemic administration of peptide with apurinic/apyrimidinic endonuclease activity inhibits induction of cell death after focal cerebral ischemia/reperfusion in mice., PMID:19481583
[Benzonase--possibility of practical application]., PMID:19514462
Polymerase chain reaction, nuclease digestion, and mass spectrometry based assay for the trinucleotide repeat status of the fragile X mental retardation 1 gene., PMID:19514725
Chlorambucil-adducts in DNA analyzed at the oligonucleotide level using HPLC-ESI MS., PMID:19621941
Evaluation of the Serratia marcescens nuclease (NucA) as a transgenic cell ablation system in porcine., PMID:19937494
Labelling of live cells using fluorescent aptamers: binding reversal with DNA nucleases., PMID:20388214
Clearance and characterization of residual HSV DNA in recombinant adeno-associated virus produced by an HSV complementation system., PMID:20668485
Analysis of porcine circovirus type 1 detected in Rotarix vaccine., PMID:21093497
In situ photodegradation of incorporated polyanion does not alter prion infectivity., PMID:21304885
Manufacture of measles viruses., PMID:21590404
A BEN-domain-containing protein associates with heterochromatin and represses transcription., PMID:21914818
[Expression, purification and characterization of non-specific Serratia nuclease in Escherichia coli]., PMID:22097815
A simple and effective method to generate lentiviral vectors for ex vivo gene delivery to mature human peripheral blood lymphocytes., PMID:22515320
Affinity purification of influenza virus ribonucleoprotein complexes from the chromatin of infected cells., PMID:22688655
Rapid genome-scale mapping of chromatin accessibility in tissue., PMID:22734930
Downstream processing of lentiviral vectors: releasing bottlenecks., PMID:22934827
Structures of oxaliplatin-oligonucleotide adducts from DNA., PMID:23019159
Systematic functional comparative analysis of four single-stranded DNA-binding proteins and their affection on viral RNA metabolism., PMID:23365690
Removing residual DNA from Vero-cell culture-derived human rabies vaccine by using nuclease., PMID:25108516
Sequence-specific nuclease-mediated release of cells tethered by oligonucleotide phospholipids., PMID:25890730
An ameliorative protocol for the quantification of purine 5',8-cyclo-2'-deoxynucleosides in oxidized DNA., PMID:26284235
[Transgenic Expression of Serratia marcescens Native and Mutant Nucleases Modulates Tobacco Mosaic Virus Resistance in Nicotiana tabacum L]., PMID:26410939
Biodegradable DNA-enabled poly(ethylene glycol) hydrogels prepared by copper-free click chemistry., PMID:26541212
Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins., PMID:26821228
ChIP-Sequencing to Map the Epigenome of Senescent Cells Using Benzonase Endonuclease., PMID:27423868
On the utility of the benzonase treatment for correct laboratory diagnosis of parvovirus B19 infection., PMID:28822317
Improving Fab' fragment retention in an autonucleolytic Escherichia coli strain by swapping periplasmic nuclease translocation signal from OmpA to DsbA., PMID:28875244
In Situ Tissue Labeling of Cerebral Amyloid Using HIV-Related Tat Peptide., PMID:29349578
Performance of pre-RT-qPCR treatments to discriminate infectious human rotaviruses and noroviruses from heat-inactivated viruses: applications of PMA/PMAxx, benzonase and RNase., PMID:29464825
Endonuclease from Gram-Negative Bacteria Serratia marcescens Is as Effective as Pulmozyme in the Hydrolysis of DNA in Sputum., PMID:29503617
Application of benzonase in preparation of decellularized lamellar porcine corneal stroma for lamellar keratoplasty., PMID:31330094
[Vaccine pretreatment for quantification of 146S antigen in foot-and-mouth disease vaccines by high performance size exclusion chromatography]., PMID:31441615
Decellularized pig pulmonary heart valves-Depletion of nucleic acids measured by proviral PERV pol., PMID:31692126
In vitro preparation of uniform and nucleic acid free hepatitis B core particles through an optimized disassembly-purification-reassembly process., PMID:32898688
A novel cell permeability assay for macromolecules., PMID:33126861
Enzymatic biofilm destabilisation to support mechanical cleansing of inserted dental implant surfaces: an in-vitro pilot study., PMID:33740161
Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA., PMID:34039428
A double-tetrahedral DNA framework based electrochemical biosensor for ultrasensitive detection and release of circulating tumor cells., PMID:34585683
Nondestructive separation/enrichment and rolling circle amplification-powered sensitive SERS enumeration of circulating tumor cells via aptamer recognition., PMID:37054599
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis., PMID:37683822
Nuclear growth and import can be uncoupled., PMID:37903226
Engineering an Autonucleolytic Mammalian Suspension Host Cell Line to Reduce DNA Impurity Levels in Serum-Free Lentiviral Process Streams., PMID:38266181
Electrochemical cytosensor utilizing tetrahedral DNA/bimetallic AuPd holothurian-shaped nanoparticles for ultrasensitive non-destructive detection of circulating tumor cells., PMID:38709403
Removal and monitoring of residual nucleic acids from core streptavidin inclusion bodies for increased refolding yield., PMID:39181482
An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity., PMID:39257529
Highly pure measles virus generated by combination of salt-active nuclease treatment and heparin affinity chromatography., PMID:39488125
Protein engineering enables Serratia marcescens nuclease A to hydrolyze nucleic acids under high-salt conditions., PMID:39566763
Advances in preparation of acellular human dermis for tissue banking and transplantation., PMID:39653869
Optimizing nuclease treatment to enhance anion exchange chromatography of HIV-derived virus-like particles., PMID:40056795
For research use only. Not for human or drug use.
