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Recombinant ASFV p54/pE183L Protein, N-His (YVV16001)

Applications:ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system:E. coli
Accession:R4S7F0
Protein length:Met1-Leu133
Overview

Catalog No.

YVV16001

Expression system

E. coli

Species

African swine fever virus (ASFV)

Protein length

Met1-Leu133

Predicted molecular weight

16.26 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

R4S7F0

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Inner membrane protein p54, pE183L

Data Image
  • SDS-PAGE
    SDS PAGE for recombinant ASFV p54/pE183L Protein
References

Epitope mapping of African swine fever virus (ASFV) structural protein, p54. PMID: 32004574

HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies. PMID: 35612629

Lactobacillus plantarum surface-displayed ASFV (p54) with porcine IL-21 generally stimulates protective immune responses in mice. PMID: 34383171

An extensive evaluation of codon usage pattern and bias of structural proteins p30, p54 and, p72 of the African swine fever virus (ASFV). PMID: 34901328

Development of a novel real-time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam. PMID: 34388311

Roles of African Swine Fever Virus Structural Proteins in Viral Infection. PMID: 29978065

Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes. PMID: 35498728

African swine fever virus: A raised global upsurge and a continuous threaten to pig husbandry. PMID: 35526679

Development and characterization of monoclonal antibodies against the N-terminal domain of African swine fever virus structural protein, p54. PMID: 33737177

Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus. PMID: 36366433

Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay. PMID: 35698809

Computational analysis of immunogenic epitopes in the p30 and p54 proteins of African swine fever virus. PMID: 36148815

Porcine Immunoglobulin Fc Fused P30/P54 Protein of African Swine Fever Virus Displaying on Surface of S. cerevisiae Elicit Strong Antibody Production in Swine. PMID: 32915442

Identification of a Linear B Cell Epitope on p54 of African Swine Fever Virus Using Nanobodies as a Novel Tool. PMID: 37191526

Characterization of Anti-p54 Monoclonal Antibodies and Their Potential Use for African Swine Fever Virus Diagnosis. PMID: 33562314

The structural protein p54 is essential for African swine fever virus viability. PMID: 8725112

A Novel Linear B-Cell Epitope on the P54 Protein of African Swine Fever Virus Identified Using Monoclonal Antibodies. PMID: 37112846

Identification of one novel epitope targeting p54 protein of African swine fever virus using monoclonal antibody and development of a capable ELISA. PMID: 34638027

ASFV proteins presented at the surface of T7 phages induce strong antibody responses in mice. PMID: 36965632

ASFV in Tanzania: asymptomatic pigs harbor virus of molecular similarity to Georgia 2007. PMID: 23398669

Characterization and molecular basis of heterogeneity of the African swine fever virus envelope protein p54. PMID: 7933107

The African swine fever virus dynein-binding protein p54 induces infected cell apoptosis. PMID: 15225638

Indirect ELISA Using Multi-Antigenic Dominants of p30, p54 and p72 Recombinant Proteins to Detect Antibodies against African Swine Fever Virus in Pigs. PMID: 36560663

Molecular profile of African swine fever virus (ASFV) circulating in Vietnam during 2019-2020 outbreaks. PMID: 33454861

Molecular characterization of African Swine fever viruses in Burkina Faso, Mali, and Senegal 1989-2016: Genetic diversity of ASFV in West Africa. PMID: 34323385

Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide. PMID: 34715492

Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes. PMID: 19009341

Rapid and ultra-sensitive detection of African swine fever virus antibody on site using QDM based-ASFV immunosensor (QAIS). PMID: 34815032

Screening and identification of the dominant antigens of the African swine fever virus. PMID: 37215478

African swine fever virus structural protein p54 is essential for the recruitment of envelope precursors to assembly sites. PMID: 15047843

Multiple variants of African swine fever virus circulating in Vietnam. PMID: 35190886

Structural models of DYNLL1 with interacting partners: African swine fever virus protein p54 and postsynaptic scaffolding protein gephyrin. PMID: 21094642

Expression and Immunogenicity of Recombinant African Swine Fever Virus Proteins Using the Semliki Forest Virus. PMID: 35615248

Differential antibody responses in sows and finishing pigs naturally infected with African swine fever virus under field conditions. PMID: 34799123

Symptomatic and asymptomatic cases of African swine fever in Tanzania. PMID: 31325213

Splenic-targeting biomimetic nanovaccine for elevating protective immunity against virus infection. PMID: 36463277

Molecular Characterization of African Swine Fever Virus From 2019-2020 Outbreaks in Guangxi Province, Southern China. PMID: 35782548

Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum. PMID: 34454001

African swine fever virus protein p54 interacts with the microtubular motor complex through direct binding to light-chain dynein. PMID: 11559815

Rapid and visual detection of African swine fever virus antibody by using fluorescent immunochromatography test strip. PMID: 32887174

Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera. PMID: 16490226

Development of optimized protocol for culturing African swine fever virus field isolates in MA104 cells. PMID: 36211218

Flow cytometry-based multiplexing antibody detection for diagnosis of African swine fever virus. PMID: 36038241

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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For research use only. Not for human or drug use.

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Recombinant ASFV p54/pE183L Protein, N-His [YVV16001]
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