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Recombinant VSV G protein/Glycoprotein G Protein, N-His

Catalog #:   YVV09801 Specific References (116) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: B5AGV3
Protein length: Lys17-Ser467
Overview

Catalog No.

YVV09801

Expression system

E. coli

Species

Vesicular stomatitis Indiana virus (VSIV)

Protein length

Lys17-Ser467

Predicted molecular weight

52.73 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

B5AGV3

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

G protein, Glycoprotein G,VSVg,VSVG Protein,VSIV protein

Data Image
  • SDS-PAGE
    SDS PAGE for Recombinant VSV G protein/Glycoprotein G Protein
References

Baboon envelope pseudotyped LVs outperform VSV-G-LVs for gene transfer into early-cytokine-stimulated and resting HSCs. PMID: 24951430

Lentiviral Vector Pseudotypes: Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. PMID: 32933033

VSV-G Viral Envelope Glycoprotein Prepared from Pichia pastoris Enhances Transfection of DNA into Animal Cells. PMID: 28297751

Intracellular appearance of a glycoprotein in VSV-infected BHK cells lacking the membrane-anchoring oligopeptide of the viral G-protein. PMID: 6086325

Helper virus-free HSV-1 vectors packaged both in the presence of VSV G protein and in the absence of HSV-1 glycoprotein B support gene transfer into neurons in the rat striatum. PMID: 11704887

The effects of N-terminal insertion into VSV-G of an scFv peptide. PMID: 16948856

Generation of VSV pseudotypes using recombinant ΔG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines. PMID: 20709108

Preparation of vesicular stomatitis virus-G (VSV-G) conjugate and its use in gene transfer. PMID: 22474657

The glycoprotein G of rhabdoviruses. PMID: 7605197

Leucine-rich repeat-containing G protein-coupled receptor 4 facilitates vesicular stomatitis virus infection by binding vesicular stomatitis virus glycoprotein. PMID: 28842478

Vesicular stomatitis virus G protein transmembrane region is crucial for the hemi-fusion to full fusion transition. PMID: 30006542

Reconstituted G protein-lipid vesicles from vesicular stomatitis virus and their inhibition of VSV infection. PMID: 6247354

VSV G protein induces murine cytolytic T lymphocytes. PMID: 2854599

VSV-G envelope glycoprotein forms complexes with plasmid DNA and MLV retrovirus-like particles in cell-free conditions and enhances DNA transfection. PMID: 11545614

Structural basis for the recognition of LDL-receptor family members by VSV glycoprotein. PMID: 29531262

Vesicular stomatitis virus glycoprotein suppresses nuclear factor kappa-B- and mitogen-activated protein kinase-mediated pro-inflammatory responses dependent on sialic acids. PMID: 32126199

Characterization of Antibody Interactions with the G Protein of Vesicular Stomatitis Virus Indiana Strain and Other Vesiculovirus G Proteins. PMID: 30232190

Folding of VSV G protein: sequential interaction with BiP and calnexin. PMID: 7939687

Intracellular transport of VSV G protein occurs in cells lacking a nuclear envelope. PMID: 2833898

Fusion-active glycoprotein G mediates the cytotoxicity of vesicular stomatitis virus M mutants lacking host shut-off activity. PMID: 20631091

Production of vesicular stomatitis virus G glycoprotein (VSV-G) pseudotyped retroviral vectors. PMID: 18428407

Complementation of a vesicular stomatitis virus glycoprotein G mutant with wild-type protein expressed from either a bovine papilloma virus or a vaccinia virus vector system. PMID: 2171187

Inhibition of vesicular stomatitis virus infection by spike glycoprotein. Evidence for an intracellular, G protein-requiring step. PMID: 6247355

Characterization of complete particles (VSV-G/SIN-GFP) and empty particles (VSV-G/EMPTY) in human immunodeficiency virus type 1-based lentiviral products for gene therapy: potential applications for improvement of product quality and safety. PMID: 18412516

Mechanism of membrane fusion induced by vesicular stomatitis virus G protein. PMID: 27974607

Factors that influence VSV-G pseudotyping and transduction efficiency of lentiviral vectors-in vitro and in vivo implications. PMID: 17366519

Plasma membrane microdomains containing vesicular stomatitis virus M protein are separate from microdomains containing G protein and nucleocapsids. PMID: 18367537

The G protein of vesicular stomatitis virus has free access into and egress from the smooth endoplasmic reticulum of UT-1 cells. PMID: 2155242

p38: A novel protein that associates with the vesicular stomatitis virus glycoprotein. PMID: 11554768

Import of hybrid vesicular stomatitis G protein to the mitochondrial inner membrane. PMID: 3031055

The Engineered MARCH8-Resistant Vesicular Stomatitis Virus Glycoprotein Enhances Lentiviral Vector Transduction. PMID: 33678011

Glycoprotein-dependent acidification of vesicular stomatitis virus enhances release of matrix protein. PMID: 19776119

Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors. PMID: 23364315

Infectivity of a human respiratory syncytial virus lacking the SH, G, and F proteins is efficiently mediated by the vesicular stomatitis virus G protein. PMID: 12610153

Intracellular transport of the glycoprotein of VSV is inhibited by CCCP at a late stage of post-translational processing. PMID: 2557359

A sorting signal for the basolateral delivery of the vesicular stomatitis virus (VSV) G protein lies in its luminal domain: analysis of the targeting of VSV G-influenza hemagglutinin chimeras. PMID: 2542964

Vesicular stomatitis virus glycoprotein containing the entire green fluorescent protein on its cytoplasmic domain is incorporated efficiently into virus particles. PMID: 11162797

Analysis of protein transport through the Golgi in a reconstituted cell-free system. PMID: 1901603

Refined methods for propagating vesicular stomatitis virus vectors that are defective for G protein expression. PMID: 19941901

A GP64-null baculovirus pseudotyped with vesicular stomatitis virus G protein. PMID: 11222677

Generation of High-Titer Pseudotyped Lentiviral Vectors. PMID: 30706393

Polarized expression of a chimeric protein in which the transmembrane and cytoplasmic domains of the influenza virus hemagglutinin have been replaced by those of the vesicular stomatitis virus G protein. PMID: 3025835

Vesicular stomatitis virus growth in Drosophila melanogaster cells: G protein deficiency. PMID: 6245242

Membrane fusion activity of vesicular stomatitis virus glycoprotein G is induced by low pH but not by heat or denaturant. PMID: 12781719

Pseudotyping of glycoprotein D-deficient herpes simplex virus type 1 with vesicular stomatitis virus glycoprotein G enables mutant virus attachment and entry. PMID: 10666285

Truncated vesicular stomatitis virus G protein improves baculovirus transduction efficiency in vitro and in vivo. PMID: 16267571

Pseudotype formation of murine leukemia virus with the G protein of vesicular stomatitis virus. PMID: 1847450

Efficient export of the vesicular stomatitis virus G protein from the endoplasmic reticulum requires a signal in the cytoplasmic tail that includes both tyrosine-based and di-acidic motifs. PMID: 10637287

Structures of vesicular stomatitis virus glycoprotein: membrane fusion revisited. PMID: 18345480

A VSV-G pseudotyped HIV vector mediates efficient transduction of human pulmonary artery smooth muscle cells. PMID: 11220675

Cell-cell transmission of VSV-G pseudotyped lentivector particles. PMID: 24040363

Mutational analysis of the vesicular stomatitis virus glycoprotein G for membrane fusion domains. PMID: 8389917

Unusual heterogeneity in the glycosylation of the G protein of the hazelhurst strain of vesicular stomatitis virus. PMID: 6314902

Amphotropic and VSV-G-pseudotyped retroviral vectors transduce human hematopoietic progenitor cells with similar efficiency. PMID: 10933195

Vesicular Stomatitis Virus glycoprotein G carrying a tandem dimer of Foot and Mouth Disease Virus antigenic site A can be used as DNA and peptide vaccine for cattle. PMID: 21889542

The HIV-1 Env protein signal sequence retards its cleavage and down-regulates the glycoprotein folding. PMID: 10873786

N-terminal domain of Borna disease virus G (p56) protein is sufficient for virus receptor recognition and cell entry. PMID: 11435588

VSV-G-pseudotyped retroviral packaging through adenovirus-mediated inducible gene expression. PMID: 9125185

Morphological analysis of the transfer of VSV ts-045 G glycoprotein from the endoplasmic reticulum to the intermediate compartment in vero cells. PMID: 8831570

Traffic through the Golgi apparatus. PMID: 11756463

Using temperature-sensitive mutants of VSV to study membrane protein biogenesis. PMID: 2558277

Characterization of the Bas-Congo virus glycoprotein and its function in pseudotyped viruses. PMID: 23785218

Glycosylation is not required for the fusion activity of the G protein of vesicular stomatitis virus in insect cells. PMID: 2650461

The HIV envelope but not VSV glycoprotein is capable of mediating HIV latent infection of resting CD4 T cells. PMID: 19851458

Pseudotransduction of hepatocytes by using concentrated pseudotyped vesicular stomatitis virus G glycoprotein (VSV-G)-Moloney murine leukemia virus-derived retrovirus vectors: comparison of VSV-G and amphotropic vectors for hepatic gene transfer. PMID: 8642678

The glycoprotein of VSV accumulates in a distal Golgi compartment in the presence of CCCP. PMID: 2557360

A New Vaccine Candidate Expressing JUNV GP1-GP2 Against Argentine Hemorrhagic Fever Based on Baculovirus Surface Display., PMID:40514553

VSV-CHIKV activates antitumor immunity by inducing pyroptosis in a melanoma model., PMID:40439822

Comparison of the induction of neutralizing antibodies against Bas congo virus using several vaccine modalities., PMID:40348847

Engineering of extracellular vesicles for efficient intracellular delivery of multimodal therapeutics including genome editors., PMID:40301355

Membrane-targeted immunogenic compositions using exosome mimetic approach for vaccine development against SARS-CoV-2 and other pathogens., PMID:40157987

A self-adjuvant multiantigenic nanovaccines simultaneously activate the antiviral and antitumor immunity for the treatment of cancers., PMID:40016753

Itaconate facilitates viral infection via alkylating GDI2 and retaining Rab GTPase on the membrane., PMID:39730330

Capturing intermediates and membrane remodeling in class III viral fusion., PMID:39630917

Assessment of Single-Cycle M-Protein Mutated Vesicular Stomatitis Virus as a Safe and Immunogenic Mucosal Vaccine Platform for SARS-CoV-2 Immunogen Delivery., PMID:39526809

Comprehensive untargeted lipidomic profiling of third generation lentiviral vectors and packaging cells., PMID:39378046

Tuning VSV-G Expression Improves Baculovirus Integrity, Stability and Mammalian Cell Transduction Efficiency., PMID:39339951

Guanylate-binding protein 5 antagonizes viral glycoproteins independently of furin processing., PMID:39212413

Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing., PMID:39205190

Efficacy and Immunogenicity of a Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine in Cynomolgus Macaques., PMID:39205155

Viral and cellular determinants of polarized trafficking of viral envelope proteins from insect-specific and insect-vectored viruses in insect midgut and salivary gland cells., PMID:39162433

AP3B1 facilitates PDIA3/ERP57 function to regulate rabies virus glycoprotein selective degradation and viral entry., PMID:39128851

The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins., PMID:38932120

Product Enhanced Reverse Transcriptase for assessing replication competent virus in vectors retroviral vectors pseudotyped with GALV and VSV-G envelopes., PMID:38908550

Chandipura viral glycoprotein (CNV-G) promotes Gectosome generation and enables delivery of intracellular therapeutics., PMID:38702887

Development and Validation of a Plaque Assay to Determine the Titer of a Recombinant Live-Attenuated Viral Vaccine for SARS-CoV-2., PMID:38675756

Optimizing lentiviral vector formulation conditions for efficient ex vivo transduction of primary human T cells in chimeric antigen receptor T-cell manufacturing., PMID:38661611

Enhanced production yields of rVSV-SARS-CoV-2 vaccine using Fibra-Cel® macrocarriers., PMID:38449674

Development of a stable Sf9 insect cell line to produce VSV-G pseudotyped baculoviruses., PMID:38278988

CD97 negatively regulates the innate immune response against RNA viruses by promoting RNF125-mediated RIG-I degradation., PMID:37978243

Peptide ligands targeting the vesicular stomatitis virus G (VSV-G) protein for the affinity purification of lentivirus particles., PMID:37947118

Adaptation of an rVSV Ebola vaccine purification process for rapid development of a viral vaccine candidate for SARS-CoV-2., PMID:37766672

Efficient generation and characterization of chimeric dengue viral-like particles., PMID:36878035

Understanding the Spike Protein in COVID-19 Vaccine in Recombinant Vesicular Stomatitis Virus (rVSV) Using Automated Capillary Western Blots., PMID:36685032

The interaction of viral fusion peptides with lipid membranes., PMID:36110043

Codon-optimization of the respiratory syncytial virus (RSV) G protein expressed in a vesicular stomatitis virus (VSV) vector improves immune responses in a cotton rat model., PMID:36096069

Molecular insights into the regulation of constitutive activity by RNA editing of 5HT2C serotonin receptors., PMID:35977511

Characterization of Macrophage-Tropic HIV-1 Infection of Central Nervous System Cells and the Influence of Inflammation., PMID:35975998

Efficient Gene Transfer to Kidney Using a Lentiviral Vector Pseudotyped with Zika Virus Envelope Glycoprotein., PMID:35904396

Bovine lactoferrin increases the poly(I:C)-induced antiviral response in vitro., PMID:35830725

Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus., PMID:35755944

A single dose of recombinant VSV-RABVG vaccine provides full protection against RABV challenge., PMID:35618603

Boosting of SARS-CoV-2 immunity in nonhuman primates using an oral rhabdoviral vaccine., PMID:35282925

Immunogenicity of a recombinant VSV-Vectored SARS-CoV vaccine induced robust immunity in rhesus monkeys after single-dose immunization., PMID:35234625

Growth, Antigenicity, and Immunogenicity of SARS-CoV-2 Spike Variants Revealed by a Live rVSV-SARS-CoV-2 Virus., PMID:35071273

A vesicular stomatitis virus-based prime-boost vaccination strategy induces potent and protective neutralizing antibodies against SARS-CoV-2., PMID:34914812

Cellular Proteo-Transcriptomic Changes in the Immediate Early-Phase of Lentiviral Transduction., PMID:34835333

Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies., PMID:34822651

Strategies for all-at-once and stepwise selection of cells with multiple genetic manipulations., PMID:34695756

Spike protein of SARS-CoV-2 activates macrophages and contributes to induction of acute lung inflammation in male mice., PMID:34365657

Lentiviral transduction of neonatal rat ventricular myocytes preserves ultrastructural features of genetically modified cells., PMID:34365094

Metastable biomolecular condensates of interferon-inducible antiviral Mx-family GTPases: A paradigm shift in the last three years., PMID:34323222

YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion., PMID:34301937

Tree Shrew Cells Transduced with Human CD4 and CCR5 Support Early Steps of HIV-1 Replication, but Viral Infectivity Is Restricted by APOBEC3., PMID:34076481

Reprogramming Extracellular Vesicles for Protein Therapeutics Delivery., PMID:34064144

Induction of protective immune responses against a lethal Zika virus challenge post-vaccination with a dual serotype of recombinant vesicular stomatitis virus carrying the genetically modified Zika virus E protein gene., PMID:33913804

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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Recombinant VSV G protein/Glycoprotein G Protein, N-His [YVV09801]
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