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Anti-PDK1/PDPK1 Polyclonal Antibody

Catalog #:   PHA56301 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O15530
Overview

Catalog No.

PHA56301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PDK1/PDPK1 (Lys83-Ala338).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PDK1,hPDK1,PDPK1,3-phosphoinositide-dependent protein kinase 1

Purification

Purified by antigen affinity column.

Accession

O15530

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with PDK1 / PDPK1 antibody (PHA56301) at 0.4μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: NCI-H460 cell lysate
    Lane 3: K562 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 48,63 kDa
    Observed MW: 48,68 kDa
  • Immunocytochemistry/ Immunofluorescence
    PDK1 / PDPK1 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with PDK1 / PDPK1 (PHA56301) at 4 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PDK1/PDPK1 antibody (PHA56301) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 32 kDa
    Observed MW: 32 kDa
References

Hypoxia inducible factor-1α drives cancer resistance to cuproptosis., PMID:40054467

NIR-II Imaging for Tracking the Spatiotemporal Immune Microenvironment in Atherosclerotic Plaques., PMID:39630481

Leucine-Rich Alpha-2-Glycoprotein 1 Promotes Metastatic Colorectal Cancer Growth Through Human Epidermal Growth Factor Receptor 3 Signaling., PMID:39393543

O-GlcNAcylation mediates Wnt-stimulated bone formation by rewiring aerobic glycolysis., PMID:39256595

Retraction Note: Long non-coding RNA FAL1 regulated cell proliferation through Akt pathway via targeting PDK1 in esophageal cancer cells., PMID:38305630

A PD-L1 Antibody-Conjugated PAMAM Dendrimer Nanosystem for Simultaneously Inhibiting Glycolysis and Promoting Immune Response in Fighting Breast Cancer., PMID:37522451

PD-L1 expression is regulated by ATP-binding of the ERBB3 pseudokinase domain., PMID:37397533

Phosphoinositol 3-kinase-driven NET formation involves different isoforms and signaling partners depending on the stimulus., PMID:36761736

Molecular classification of human papillomavirus-positive cervical cancers based on immune signature enrichment., PMID:36203656

Metabolic regulation of intrasynovial flexor tendon repair: The effects of dichloroacetate administration on early tendon healing in a canine model., PMID:35488732

GDC-0941 activates integrin linked kinase (ILK) expression to cause resistance to GDC-0941 in breast cancer by the tumor necrosis factor (TNF)-α signaling pathway., PMID:35477364

Patient pIgR-enriched extracellular vesicles drive cancer stemness, tumorigenesis and metastasis in hepatocellular carcinoma., PMID:34922977

Hypoxia, Metabolic Reprogramming, and Drug Resistance in Liver Cancer., PMID:34359884

Small molecules targeting the innate immune cGAS‒STING‒TBK1 signaling pathway., PMID:33354501

Up-regulation of FoxO1 contributes to adverse vascular remodelling in type 1 diabetic rats., PMID:33108705

CRISPR-Cas13d for Gene Knockdown and Engineering of CHO Cells., PMID:32911927

Cancer-derived IgG involved in cisplatin resistance through PTP-BAS/Src/PDK1/AKT signaling pathway., PMID:32730654

Systems analysis of protein signatures predicting cetuximab responses in KRAS, NRAS, BRAF and PIK3CA wild-type patient-derived xenograft models of metastatic colorectal cancer., PMID:32700762

Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrhea., PMID:32567324

Yokukansan, a Kampo medicine, enhances the level of neuronal lineage markers in differentiated P19 embryonic carcinoma cells., PMID:31692643

Aberrant upregulation of PDK1 in ovarian cancer cells impairs CD8+ T cell function and survival through elevation of PD-L1., PMID:31646108

Rational combination with PDK1 inhibition overcomes cetuximab resistance in head and neck squamous cell carcinoma., PMID:31578313

Associations of PGK1 promoter hypomethylation and PGK1-mediated PDHK1 phosphorylation with cancer stage and prognosis: a TCGA pan-cancer analysis., PMID:31578148

The AKT kinase signaling network is rewired by PTEN to control proximal BCR signaling in germinal center B cells., PMID:31011187

Stage-specific requirement of kinase PDK1 for NK cells development and activation., PMID:30622306

Spatial alterations of De Novo purine biosynthetic enzymes by Akt-independent PDK1 signaling pathways., PMID:29668719

JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling., PMID:29584620

Assessment of PI3K/AKT/PTEN signaling pathway activity in colorectal cancer using quantum dot-conjugated antibodies., PMID:29422975

SHORT syndrome due to a novel de novo mutation in PRKCE (Protein Kinase Cɛ) impairing TORC2-dependent AKT activation., PMID:28934384

Inhibition of SHIP2 in CD2AP-deficient podocytes ameliorates reactive oxygen species generation but aggravates apoptosis., PMID:28878342

Particular phosphorylation of PI3K/Akt on Thr308 via PDK-1 and PTEN mediates melatonin's neuroprotective activity after focal cerebral ischemia in mice., PMID:28395173

Increased expression of platelet-derived growth factor associated protein-1 is associated with PDGF-B mediated glioma progression., PMID:27448842

Chromatin remodeling gene AT-rich interactive domain-containing protein 1A suppresses gastric cancer cell proliferation by targeting PIK3CA and PDK1., PMID:27323812

Dynamic Contrast Enhanced MRI Assessing the Antiangiogenic Effect of Silencing HIF-1α with Targeted Multifunctional ECO/siRNA Nanoparticles., PMID:27264671

Prevention Effects and Possible Molecular Mechanism of Mulberry Leaf Extract and its Formulation on Rats with Insulin-Insensitivity., PMID:27054886

S6 kinase phosphorylated at T229 is involved in tau and actin pathologies in Alzheimer's disease., PMID:26582459

Double-Edge Sword of Sustained ROCK Activation in Prion Diseases through Neuritogenesis Defects and Prion Accumulation., PMID:26241960

[Metabolism enzyme controls cancer stemness]., PMID:25985625

Comprehensive analysis of glycolytic enzymes as therapeutic targets in the treatment of glioblastoma., PMID:25932951

Similar PDK1-AKT-mTOR pathway activation in balloon cells and dysmorphic neurons of type II focal cortical dysplasia with refractory epilepsy., PMID:25847349

Targeting pyruvate kinase M2 contributes to radiosensitivity of non-small cell lung cancer cells in vitro and in vivo., PMID:25444918

Protein kinase C in the wood frog, Rana sylvatica: reassessing the tissue-specific regulation of PKC isozymes during freezing., PMID:25210662

PDK1 regulates B cell differentiation and homeostasis., PMID:24979759

Effects of resveratrol on the insulin signaling pathway of obese mice., PMID:24675832

Increased 8-hydroxy-2'-deoxyguanosine in plasma and decreased mRNA expression of human 8-oxoguanine DNA glycosylase 1, anti-oxidant enzymes, mitochondrial biogenesis-related proteins and glycolytic enzymes in leucocytes in patients with systemic lupus erythematosus., PMID:24345202

Isoaspartate accumulation in mouse brain is associated with altered patterns of protein phosphorylation and acetylation, some of which are highly sex-dependent., PMID:24224061

Thyrotropin regulates IL-6 expression in CD34+ fibrocytes: clear delineation of its cAMP-independent actions., PMID:24086448

Characterization of fragmented 3-phosphoinsitide-dependent protein kinase-1 (PDK1) by phosphosite-specific antibodies., PMID:24044887

Mammalian target of rapamycin pathway activity in alveolar soft part sarcoma., PMID:23871289

Crosstalk between PI3 kinase/PDK1/Akt/Rac1 and Ras/Raf/MEK/ERK pathways downstream PDGF receptor., PMID:23817184

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-PDK1/PDPK1 Polyclonal Antibody [PHA56301]
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