Catalog No.
PHA27801
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human APAF1 (Ser31-Phe350).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
APAF1,APAF-1,Apoptotic protease-activating factor 1,KIAA0413
Purification
Purified by antigen affinity column.
Accession
O14727
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Effects of rivaroxaban on myocardial mitophagy in the rat heart., PMID:37664781
The Prognostic Significance of Apoptotic Protease Activating Factor (Apaf-1) Protein Expression in Colon Adenocarcinoma Tissue-Preliminary Report., PMID:36866552
Hepatopancreas toxicity and immunotoxicity of a fungicide, pyraclostrobin, on common carp., PMID:36030005
Systematic validation and assessment of immunohistochemical markers for central nervous system pathology in cetaceans, with emphasis on auditory pathways., PMID:35648776
Panax notoginseng saponin R1 attenuates allergic rhinitis through AMPK/Drp1 mediated mitochondrial fission., PMID:35623408
MCL-1 is a clinically targetable vulnerability in breast cancer., PMID:35349392
Calcium Hydroxide Increases Human Umbilical Cord Mesenchymal Stem Cells Expressions of Apoptotic Protease-Activating Factor-1, Caspase-3 and Caspase-9., PMID:33727863
STAT3/NF-κB signalling disruption in M2 tumour-associated macrophages is a major target of PLGA nanocarriers/PD-L1 antibody immunomodulatory therapy in breast cancer., PMID:33434950
Intracellular leucine-rich alpha-2-glycoprotein-1 competes with Apaf-1 for binding cytochrome c in protecting MCF-7 breast cancer cells from apoptosis., PMID:33386492
Low expression of pro-apoptotic proteins Bax, Bak and Smac indicates prolonged progression-free survival in chemotherapy-treated metastatic melanoma., PMID:32054850
The VP3 protein of duck hepatitis A virus mediates host cell adsorption and apoptosis., PMID:31727985
Novel Carbazole-Piperazine Hybrid Small Molecule Induces Apoptosis by Targeting BCL-2 and Inhibits Tumor Progression in Lung Adenocarcinoma in Vitro and Xenograft Mice Model., PMID:31450709
Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system., PMID:30004576
Blockade of ITGA2 Induces Apoptosis and Inhibits Cell Migration in Gastric Cancer., PMID:29743821
Starfish Apaf-1 activates effector caspase-3/9 upon apoptosis of aged eggs., PMID:29371610
Protective Effect of Combined Caffeic Acid Phenethyl Ester and Bevacizumab Against Hydrogen Peroxide-Induced Oxidative Stress in Human RPE Cells., PMID:28937872
The natural flavonoid apigenin sensitizes human CD44+ prostate cancer stem cells to cisplatin therapy., PMID:28107698
Antibody-based proteomics to identify an apoptosis signature for early recurrence of hepatocellular carcinoma., PMID:27799868
The effects of tadalafil and pentoxifylline on apoptosis and nitric oxide synthase in liver ischemia/reperfusion injury., PMID:27450022
Sperm-associated antigen 9 (SPAG9) promotes the survival and tumor growth of triple-negative breast cancer cells., PMID:27449044
Activated caspase-9 immunoreactivity in glial and neuronal cytoplasmic inclusions in multiple system atrophy., PMID:27345387
Altered S-nitrosothiol homeostasis provides a survival advantage to breast cancer cells in HER2 tumors and reduces their sensitivity to trastuzumab., PMID:26854735
Association between early promoter-specific DNA methylation changes and outcome in older acute myeloid leukemia patients., PMID:26818573
Mitochondrial protection impairs BET bromodomain inhibitor-mediated cell death and provides rationale for combination therapeutic strategies., PMID:26658189
DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway., PMID:25779535
Bortezomib enhances the therapeutic efficacy of dasatinib by promoting c-KIT internalization-induced apoptosis in gastrointestinal stromal tumor cells., PMID:25737303
The E3 ligase PARC mediates the degradation of cytosolic cytochrome c to promote survival in neurons and cancer cells., PMID:25028717
The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice., PMID:24763053
Toward personalized therapy for chronic lymphocytic leukemia: DSC and cDNA microarray assessment of two cases., PMID:23114648
[Molecular and morphological aspects of endometrial receptivity disorders at chronic endometritis]., PMID:22937573
The apoptotic function analysis of p53, Apaf1, Caspase3 and Caspase7 during the spermatogenesis of the Chinese fire-bellied newt Cynops orientalis., PMID:22768170
Ontogeny of Lafora bodies and neurocytoskeleton changes in Laforin-deficient mice., PMID:22542948
Curcumin induces Apaf-1-dependent, p21-mediated caspase activation and apoptosis., PMID:22101335
Erythropoietin and Wnt1 govern pathways of mTOR, Apaf-1, and XIAP in inflammatory microglia., PMID:22023617
The synthesized novel fluorinated compound (LJJ-10) induces death receptor- and mitochondria-dependent apoptotic cell death in the human osteogenic sarcoma U-2 OS cells., PMID:21514013
Age-dependent rat retinal ganglion cell susceptibility to apoptotic stimuli: implications for glaucoma., PMID:21489112
Mechanism of tyrosine phosphorylation of procaspase-9 and Apaf-1 in cytosolic fractions of the cerebral cortex of newborn piglets during hypoxia., PMID:20570712
Effect of dexmedetomidine on testicular torsion/detorsion damage in rats., PMID:20173379
The cleaved-Caspase-3 antibody is a marker of Caspase-9-like DRONC activity in Drosophila., PMID:19960024
Cytochrome c-induced lymphocyte death from the outside in: inhibition by serum leucine-rich alpha-2-glycoprotein-1., PMID:19851871
Caspase-9 activation by the apoptosome is not required for fas-mediated apoptosis in type II Jurkat cells., PMID:19758996
A chemical inhibitor of Apaf-1 exerts mitochondrioprotective functions and interferes with the intra-S-phase DNA damage checkpoint., PMID:19152031
Functional genetic screening reveals the role of mitochondrial cytochrome b as a mediator of FAS-induced apoptosis., PMID:18796602
Rapid and significant induction of TRAIL-mediated type II cells in apoptosis of primary salivary epithelial cells in primary Sjögren's syndrome., PMID:18791828
TIMP-1-GPI in combination with hyperthermic treatment of melanoma increases sensitivity to FAS-mediated apoptosis., PMID:18618109
Deregulation of the mitochondrial apoptotic machinery and development of molecular targeted drugs in acute myeloid leukemia., PMID:18473734
Required roles of Bax and JNKs in central and peripheral nervous system death of retinoblastoma-deficient mice., PMID:17984095
Sera of patients with celiac disease and neurologic disorders evoke a mitochondrial-dependent apoptosis in vitro., PMID:17631142
Modification of gene products involved in resistance to apoptosis in metastatic colon cancer cells: roles of Fas, Apaf-1, NFkappaB, IAPs, Smac/DIABLO, and AIF., PMID:17603079
Requirement of Apaf-1 for mitochondrial events and the cleavage or activation of all procaspases during genotoxic stress-induced apoptosis., PMID:17348858
For research use only. Not for human or drug use.
