Catalog No.
RGK23001
Description
The antibody is specific for digoxin, a cardiac glycoside derived from the foxglove plant. Digoxin is administered for the therapeutic treatment of heart conditions such as atrial fibrillation, atrial flutter and heart failure, an acts by inhibiting the Na+/K+ ATPase. However, the drug has a very narrow therapeutic index and can cause toxic side effects. The Fab fragment prepared from 26-10 that neutralises the activity of digoxin and can be used as an antidote in case of overdose of digitalis. Anti-digoxin monoclonal antibodies can also be used to measure serum levels of the drug as a guide to therapy, and are useful in the investigation of the kinetics of interaction between antibody and antigen.
Host species
Mouse
Isotype
IgG2a, kappa
Clonality
Monoclonal
Target
Digoxin, DGX, CAS: 20830-75-5,digoxinum,12β-hydroxydigitoxin,ChEBI: 4551, 26-10#, 26G10
Concentration
2.19 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Applications
CLIA, DB, ELISA, IP, RIA, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
26G10
Human Deaths Related to Oleander Poisoning: A Review of the Literature., PMID:40137888
Low-background CRISPR/Cas12a sensing system with circular CRISPR RNA for amplified fluorescent detection of antibody in human serum., PMID:39961250
Should digoxin immune fab be administered based solely on reported ingested amount in acute digoxin poisoning?, PMID:39848856
Acute oral digoxin in healthy adults hastens fatigue and increases plasma K+ during intense exercise, despite preserved skeletal muscle Na+,K+-ATPase., PMID:39580613
Analyte-induced hindrance in the RCA-assisted CRISPR/Cas12a system for homogeneous protein assays., PMID:39489975
An electrochemical proximity assay (ECPA) for antibody detection incorporating flexible spacers for improved performance., PMID:39367148
Expert Consensus on the Diagnosis and Management of Digoxin Toxicity., PMID:39265879
CRISPR/Cas12a coupled with loop-mediated isothermal amplification and lateral flow assay for SARS-CoV-2 detection., PMID:39158842
Bidirectional ventricular tachycardia due to digoxin-diuretic interaction in post-cardiac surgery patient: a case report., PMID:39015194
Visual Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid On-Site Detection of Escherichia coli O157: H7 in Milk Products., PMID:38998648
Target-responsive triplex aptamer nanoswitch enables label-free and ultrasensitive detection of antibody in human serum via lighting-up RNA aptamer transcriptions., PMID:38917548
Factors affecting the expression and stability of full-length and truncated SRSF3 proteins in human cancer cells., PMID:38909100
Not only Van Gogh: a case of BRASH syndrome with concomitant digoxin toxicity., PMID:38851740
Antibody-Bridged DNAzyme Walker for Sensitive Detection of Small Molecules., PMID:38598690
Development of nucleic acid lateral flow immunoassay for molecular detection of Entamoeba moshkovskii and Entamoeba dispar in stool samples., PMID:38503871
Plasma exchange for the management of digoxin toxicity in an individual with an acute kidney injury: A case report., PMID:38499459
Spironolactone metabolite causes falsely increased progesterone in the Abbott Architect immunoassay., PMID:38484829
Successful management of massive digoxin overdose using DIGIFab and therapeutic plasma exchange: a case report., PMID:38439066
A suicide attempt by ingestion of oleander leaves and treatment with digoxin-specific Fab antibody fragments., PMID:38146758
A novel universal small-molecule detection platform based on antibody-controlled Cas12a switching., PMID:38064994
Application of N-Terminal Site-Specific Biotin and Digoxigenin Conjugates to Clinical Anti-drug Antibody Assay Development., PMID:38050929
Efficient and rapid linker optimization with heterodimeric coiled coils improves the response of fluorescent biosensors comprising antibodies and protein M., PMID:37941425
Laurel but Hardy: unintended poisoning, a case report of oleander misidentification as bay laurel., PMID:37885923
Digoxin-specific antibodies: a novel dosing strategy., PMID:37861975
Characteristics of digoxin toxicity attended in Spanish emergency departments according to type of poisoning and administration of digoxin antibodies: the DIGITOX study., PMID:37801414
CRISPR/Cas12a-Powered Competitive Immunosorbent Assay for Small Molecules., PMID:37722021
2023 American Heart Association Focused Update on the Management of Patients With Cardiac Arrest or Life-Threatening Toxicity Due to Poisoning: An Update to the American Heart Association Guidelines for Cardiopulmonary Resuscitation and Emergency Cardiovascular Care., PMID:37721023
Rapid and portable bunyavirus SFTSV RNA testing utilizing catalytic hairpin assembly coupled with lateral flow immunoassay., PMID:37681992
Diagnosis and practical management of digoxin toxicity: a narrative review and consensus., PMID:37650725
Physiologically based pharmacokinetic model to predict drug-drug interactions with the antibody-drug conjugate enfortumab vedotin., PMID:37632598
Persistent Cardioactive Glycosides Intoxication Related to Gastric Phytobezoar of Oleander Leaves., PMID:37554479
Bradycardia, Renal Failure, Atrioventricular Nodal Blockade, Shock and Hyperkalaemia Syndrome Involving Digoxin Toxicity: A Case Report., PMID:37456352
Tissue-Specific RNA Localization in the Uterus During Implantation, Decidualization and Placentation: Technical Nuances of Various Labeling Approaches., PMID:37345986
Chronic Digoxin Toxicity: An Evaluation of Digoxin-Specific Antibodies and Other Management Options., PMID:37292530
Entrapment of Digoxin-KLH Conjugate in Alginate/Chitosan Nanoparticles: A New Antigen Delivery System For Production of Anti-digoxin Antibodies., PMID:37218193
Immunosensor for Rapid and Sensitive Detection of Digoxin., PMID:37151524
A Versatile Integrated Microfluidic Chip Based on Sonic Toothbrush-Assisted Mixing for Analyses of Diverse Biomolecules., PMID:37138452
Sensitive CRISPR-Cas12a-Assisted Immunoassay for Small Molecule Detection in Homogeneous Solution., PMID:37079720
A Case of Tachycardia-Induced Cardiomyopathy During Pregnancy: Clinical Presentation and Management., PMID:36733546
Significant Improvement in Digoxin Immunoassays Over Four Decades: Newer Assays are Less Affected by Interferences., PMID:36624574
Treatment of life-threatening digoxin toxicity with digoxin-specific antibody fragments: results from a prospective, non-interventional observational UK patient registry study., PMID:36270792
Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus., PMID:36140129
Characteristics and Outcomes of Suspected Digoxin Toxicity and Immune Fab Treatment Over the Past Two Decades-2000-2020., PMID:36089419
Life-threatening pediatric poisoning due to ingestion of Bufo bufo toad eggs: A case report., PMID:35839868
Oral digoxin effects on exercise performance, K+ regulation and skeletal muscle Na+ ,K+ -ATPase in healthy humans., PMID:35837833
Repurposing the cardiac glycoside digoxin to stimulate myelin regeneration in chemically-induced and immune-mediated mouse models of multiple sclerosis., PMID:35809238
Monitoring free digoxin serum concentrations after injection of digoxin-specific antibody fragments in a man with chronic digoxin poisoning., PMID:35736534
Detectable Digoxin Concentrations in 3 Patients with Ramps Misadventure., PMID:35691768
For research use only. Not for human or drug use.
