Catalog No.
RGK25104
Host species
Mouse
Isotype
IgG2a, kappa
Clonality
Monoclonal
Target
U1 small nuclear RNA, U1 RNA, U1 spliceosomal RNA, U1 snRNA
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Applications
DB, ELISA, IF, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
Z5scFv7b
Epitope regions on U1 small nuclear RNA recognized by anti-U1RNA-specific autoantibodies. PMID: 1385475
The 5' splice site: phylogenetic evolution and variable geometry of association with U1RNA. PMID: 2704616
Purification and characterization of human autoantibodies directed to specific regions on U1RNA; recognition of native U1RNP complexes. PMID: 8255767
Analysis of genomic clones of the murine U1RNA-associated 70-kDa protein reveals a high evolutionary conservation of the protein between human and mouse. PMID: 2525092
U1 small nuclear ribonucleoprotein immune complexes induce type I interferon in plasmacytoid dendritic cells through TLR7. PMID: 16368889
A diepitopic sequential oligopeptide carrier (SOCn) as mimic of the sm autoantigen: synthesis, conformation and biological assays. PMID: 11277497
Changes in levels of antibodies against the 70 kDa and a polypeptides of the U1RNP complex in relation to exacerbations of systemic lupus erythematosus. PMID: 1830337
A SmD peptide induces better antibody responses to other proteins within the small nuclear ribonucleoprotein complex than to SmD protein via intermolecular epitope spreading. PMID: 17277166
U1-RNP and TLR receptors in the pathogenesis of mixed connective tissue diseasePart I. The U1-RNP complex and its biological significance in the pathogenesis of mixed connective tissue disease. PMID: 27407234
Detection of autoantibodies to ribonucleoproteins by counter-immunoelectropheresis, immunoblotting and RNA-immunoprecipitation: comparison of results. PMID: 1693324
Two sisters producing anti-U1RNP exhibit serological concordance and clinical discordance. PMID: 1301988
Structural analysis of gene loci for rat U1 small nuclear RNA. PMID: 6188110
Detection and isolation of RNA-binding proteins by RNA-ligand screening of a cDNA expression library. PMID: 9380503
[Identification of the breakpoint-flanking markers on chromosomes 1 and 17 of a constitutional translocation T(1;17)(P36;Q12-21) in a patient with neuroblastoma]. PMID: 8571670
Human autoimmune serum antibodies against gag gene p30 retroviral protein also react with a U1-SnRNP 68K comigrant protein. PMID: 2960408
T cell immunity in connective tissue disease patients targets the RNA binding domain of the U1-70kDa small nuclear ribonucleoprotein. PMID: 12218166
Pulmonary function in systemic lupus erythematosus is related to distinct clinical, serologic, and nailfold capillary patterns. PMID: 1466357
Signals for the selection of a splice site in pre-mRNA. Computer analysis of splice junction sequences and like sequences. PMID: 3656413
[Detection of anti small nuclear ribonucleoprotein antibody in patients with collagen disease--a comparison of sensitivities of four methods]. PMID: 7570209
For research use only. Not for human or drug use.
