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Anti-dsDNA Antibody (3E10#) (RGK24020)

Host species:Mouse
Isotype:IgG2a, kappa
Applications:DB, ELISA
Overview

Catalog No.

RGK24020

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Target

DNA, dsDNA

Concentration

0.22 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

DB, ELISA

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

3E10#

Data Image
  • SDS-PAGE
    SDS PAGE for dsDNA Antibody
  • Dot Blot
    Dot blot analysis with Anti-dsDNA Antibody (3E10#) (RGK24020) at 1μg/ml.

    Lane 1: Plasmid ( dsDNA )

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

References

LAYN Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Gastric and Colon Cancers. PMID: 30761122

Re-engineering and evaluation of anti-DNA autoantibody 3E10 for therapeutic applications. PMID: 29374508

DNA-dependent targeting of cell nuclei by a lupus autoantibody. PMID: 26156563

Combining intracellular antibodies to restore function of mutated p53 in cancer. PMID: 26174762

A cell-penetrating antibody inhibits human RAD51 via direct binding. PMID: 29036688

Synthetic lethality of a cell-penetrating anti-RAD51 antibody in PTEN-deficient melanoma and glioma cells. PMID: 30863489

Ligation of Na, K ATPase β3 subunit on monocytes by a specific monoclonal antibody mediates T cell hypofunction. PMID: 29940031

Engagement of Na,K-ATPase beta3 subunit by a specific mAb suppresses T and B lymphocyte activation. PMID: 12456588

Na, K ATPase beta3 subunit (CD298): association with alpha subunit and expression on peripheral blood cells. PMID: 17176442

DNA mimics a self-protein that may be a target for some anti-DNA antibodies in systemic lupus erythematosus. PMID: 7836777

Laboratory evaluation of anti-dsDNA antibodies. PMID: 35016875

The Therapeutic Strategies for SLE by Targeting Anti-dsDNA Antibodies. PMID: 34542806

Self-dsDNA in the pathogenesis of systemic lupus erythematosus. PMID: 28836661

Structure and Dynamics of dsDNA in Cell-like Environments. PMID: 36359677

Direct Homologous dsDNA-dsDNA Pairing: How, Where, and Why? PMID: 31726060

Fucosylation of anti-dsDNA IgG1 correlates with disease activity of treatment-naïve systemic lupus erythematosus patients. PMID: 35182998

The Effects of Flexibility on dsDNA-dsDNA Interactions. PMID: 35629366

Anti-dsDNA: choice of assay in relation to clinical value. PMID: 1754810

Anti-double Stranded DNA Antibodies: Origin, Pathogenicity, and Targeted Therapies. PMID: 31379858

The dsDNA, Anti-dsDNA Antibody, and Lupus Nephritis: What We Agree on, What Must Be Done, and What the Best Strategy Forward Could Be. PMID: 31156647

Diameter Dependent Melting and Softening of dsDNA Under Cylindrical Confinement. PMID: 35586267

Evidence for charge-based mimicry in anti dsDNA antibody generation. PMID: 36087539

Activation of the NLRP1 inflammasome in human keratinocytes by the dsDNA mimetic poly(dA:dT). PMID: 36693106

A peptide DNA surrogate that binds and inhibits anti-dsDNA antibodies. PMID: 16290070

Clinical significance of antibodies to double stranded DNA (dsDNA) for systemic lupus erythematosus (SLE). PMID: 3304800

Evaluation of dsDNA from extracellular vesicles (EVs) in pediatric AML diagnostics. PMID: 31932899

α-2,6-sialic acid/IgG anti-dsDNA ratios correlate with human lupus disease activity and possible mechanisms: A pilot study. PMID: 35531921

Anti-dsDNA antibodies bind to TLR4 and activate NLRP3 inflammasome in lupus monocytes/macrophages. PMID: 27250627

In systemic lupus erythematosus anti-dsDNA antibodies can promote thrombosis through direct platelet activation. PMID: 31732191

Precipitating anti-dsDNA peptide repertoires in lupus. PMID: 30086185

Accumulation of cytosolic dsDNA contributes to fibroblast-like synoviocytes-mediated rheumatoid arthritis synovial inflammation. PMID: 31472320

De-sialylated and sialylated IgG anti-dsDNA antibodies respectively worsen and mitigate experimental mouse lupus proteinuria and possible mechanisms. PMID: 35640407

Recognition of DNA Target Formulations by CRISPR-Cas12a Using a dsDNA Reporter. PMID: 34142793

Anti-dsDNA antibodies in sarcoidosis. PMID: 10805357

Thermoresponsive DNA by Intercalation of dsDNA with Oligoethylene-Glycol-Functionalized Small-Molecule Intercalators. PMID: 30725517

In situ dsDNA-bevacizumab anticancer monoclonal antibody interaction electrochemical evaluation. PMID: 26526907

Anti-dsDNA antibodies bind to mesangial annexin II in lupus nephritis. PMID: 20847146

Overstretching partially alkyne functionalized dsDNA using near infrared optical tweezers. PMID: 29339160

Fibroblast growth factor inducible 14 signaling facilitates anti-dsDNA IgG penetration into mesangial cells. PMID: 32542768

Is inflammation a direct response to dsDNA breaks? PMID: 29518635

A brief note on the potential of homo-oligo-dsDNA and hetero-oligo-dsDNA based on their binder-free electrochemical characteristics on gold electrode. PMID: 33832590

Allosteric coupling between Mn2+ and dsDNA controls the catalytic efficiency and fidelity of cGAS. PMID: 32170294

Anti-dsDNA antibodies in systemic lupus erythematosus: A combination of two quantitative methods and the ANA pattern is the most efficient strategy of detection. PMID: 26407840

Protein-coated dsDNA nanostars with high structural rigidity and high enzymatic and thermal stability. PMID: 31578534

Patients with antibodies to both PmScl and dsDNA. PMID: 15517629

Interaction of antitumor flavonoids with dsDNA in the absence and presence of Cu(II). PMID: 23334220

Single-Molecule Methods for Investigating the Double-Stranded DNA Bendability. PMID: 34470919

Anti-dsDNA and anti-Sm antibodies do not predict damage in systemic lupus erythematosus. PMID: 16761503

Single Molecule Study of the Polymerization of RecA on dsDNA: The Dynamics of Individual Domains. PMID: 33842536

Sequence-dependent dsDNA-templated formation of fluorescent copper nanoparticles. PMID: 25510235

Impacts of Anti-dsDNA Antibody on In Vitro Fertilization-Embryo Transfer and Frozen-Thawed Embryo Transfer. PMID: 28540311

Concatemeric dsDNA-templated copper nanoparticles strategy with improved sensitivity and stability based on rolling circle replication and its application in microRNA detection. PMID: 24958493

Molecular fossils reveal ancient associations of dsDNA viruses with several phyla of fungi. PMID: 32071765

Anti-dsDNA antibody avidity determination by a simple reliable ELISA method for SLE diagnosis and monitoring. PMID: 12587824

Deciphering the mechanism and binding interactions of Pemetrexed with dsDNA with DNA-targeted chemotherapeutics via spectroscopic, analytical, and simulation studies. PMID: 34875572

The clinical relevance of anti-dsDNA antibodies determined by the Elia™ dsDNA assay in patients with negative indirect immunofluorescence on the HEp-2 cell. PMID: 33064668

Construction of Synthetic Nanobody Library in Mammalian Cells by dsDNA-Based Strategies*. PMID: 34411391

Construction of Peptide Library in Mammalian Cells by dsDNA-Based Strategy. PMID: 36643544

Fast and sensitive diagnosis of autoimmune disorders through amperometric biosensing of serum anti-dsDNA autoantibodies. PMID: 32469729

Ratiometric sensor based on imprinted quantum dots-cationic dye nanohybrids for selective sensing of dsDNA. PMID: 31843675

Anti-dsDNA antibodies in Brazilian patients of mainly African descent with systemic lupus erythematosus: lack of association with lupus nephritis. PMID: 19283331

A novel pathway to detect and cope with exogenous dsDNA. PMID: 27064942

Exploring the Binding Mechanism and Dynamics of EndoMS/NucS to Mismatched dsDNA. PMID: 31627318

Sae2 promotes dsDNA endonuclease activity within Mre11-Rad50-Xrs2 to resect DNA breaks. PMID: 25231868

Systemic Lupus Erythematosus with and without Anti-dsDNA Antibodies: Analysis from a Large Monocentric Cohort. PMID: 26063969

Melting dynamics of short dsDNA chains in saline solutions. PMID: 26697287

A surge in anti-dsDNA titer predicts a severe lupus flare within six months. PMID: 24316605

An improved measurement of dsDNA elasticity using AFM. PMID: 20090198

ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response. PMID: 30135424

A STING to inflammation and autoimmunity. PMID: 30990921

Goatpoxvirus ATPase activity is increased by dsDNA and decreased by zinc ion. PMID: 27146321

Prognostic value of anti-dsDNA in SLE. PMID: 6981385

The specificity of the anti-dsDNA ELISA. A closer look. PMID: 2040819

Anti-dsDNA and complement profiles as prognostic guides in systemic lupus erythematosus. PMID: 311204

Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE. PMID: 24907508

Anti-dsDNA-NcX ELISA: dsDNA-loaded nucleosomes improve diagnosis and monitoring of disease activity in systemic lupus erythematosus. PMID: 21329504

Concomitance of IgM and IgG anti-dsDNA Antibodies Does Not Appear to Associate to Active Lupus Nephritis. PMID: 24358068

Clinical evaluation of total and high-avidity anti-dsDNA antibody assays for the diagnosis of systemic lupus erythematosus. PMID: 31570052

Manganese porphyrin-dsDNA complex: a mimicking enzyme for highly efficient bioanalysis. PMID: 23427829

Anti-dsDNA antibodies cross-react with ribosomal P proteins expressed on the surface of glomerular mesangial cells to exert a cytostatic effect. PMID: 7642215

Electronic detection of dsDNA transition from helical to zipper conformation using graphene nanopores. PMID: 25325530

Interaction of calf thymus dsDNA with anti-tumor drug tamoxifen studied by zero current potentiometry. PMID: 21524901

In situ electrochemical evaluation of dsDNA interaction with the anticancer drug danusertib nitrenium radical product using the DNA-electrochemical biosensor. PMID: 26523506

Treatment with dsDNA-anti-dsDNA antibody complexes extends survival, decreases anti-dsDNA antibody production and reduces severity of nephritis in MRLlpr mice. PMID: 9116717

Single molecule identification of homology-dependent interactions between long ssRNA and dsDNA. PMID: 27580717

High Avidity dsDNA Autoantibodies in Brazilian Women with Systemic Lupus Erythematosus: Correlation with Active Disease and Renal Dysfunction. PMID: 26583157

Electrochemical, spectroscopic and molecular docking studies on the interaction of calcium channel blockers with dsDNA. PMID: 30623791

Development of anti-dsDNA autoantibodies prior to clinical diagnosis of systemic lupus erythematosus. PMID: 11439169

Comparative studies on the interaction of anticancer drug irinotecan with dsDNA and ssDNA. PMID: 35539780

Heterogeneity of anti-dsDNA antibodies in their cross-reaction with ribosomal P protein. PMID: 10585758

Fluorescent cyanine dyes for the quantification of low amounts of dsDNA. PMID: 27507117

The clinical utility of anti-double-stranded DNA antibodies and the challenges of their determination. PMID: 29807021

Association of smoking with dsDNA autoantibody production in systemic lupus erythematosus. PMID: 16150789

Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. PMID: 34703564

Defect-Induced Double-Stranded DNA Unwinding on Graphene. PMID: 33689362

[Anti-tumor effect of anti-dsDNA autoantibodies]. PMID: 15946542

International multi-center evaluation of a novel chemiluminescence assay for the detection of anti-dsDNA antibodies. PMID: 27252263

LILBID laser dissociation curves: a mass spectrometry-based method for the quantitative assessment of dsDNA binding affinities. PMID: 33230224

Serological and clinical characterization of anti-dsDNA and anti-PM/Scl double-positive patients. PMID: 17785320

Datasheet

Document Download

Anti-dsDNA Antibody (3E10#).pdf

 

$ 372
Product specifications
100 μg 372 1 mg 1860

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Anti-dsDNA Antibody (3E10#) [RGK24020]
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