Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human IL17A antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Ixekizumab in the sample competitively binds to the pre-coated protein with biotin-labeled Ixekizumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Ixekizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Catalog No.
KDH28803
Applications
Used for the quantitative determination of Ixekizumab concentration in serum, plasma and cell culture supernatant.
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.
Detection method
Colorimetric
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Sample type
Plasma, Serum
Assay type
Quantitative
Sensitivity
39.3 ng/mL
Range
78.13 - 5,000 ng/mL
Recovery
80-120%
Specifications
Ixekizumab
Alternative Names
LY2439821, CAS: 1143503-69-8
Background
Ixekizumab is a humanized monoclonal antibody to interleukin-17A which acts as an antiinflammatory agent and is used to treat moderate-to-severe plaque psoriasis. Ixekizumab has not been linked to serum enzyme elevations during therapy or to instances of idiosyncratic acute liver injury.
Shipping
2-8 ℃
Note
For Research Use Only.
For research use only. Not for human or drug use.
