Catalog No.
RGK60001
Description
DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.
Expression system
Mammalian Cells
Species reactivity
General
Host species
mouse
Isotype
IgG2a, kappa
Clonality
Monoclonal
Target
DNA-RNA Hybrid
Concentration
0.58 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Applications
CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IF, IP, SPR
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
S9.6
Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids. PMID: 33830170
Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody. PMID: 32681514
High-resolution, strand-specific R-loop mapping via S9.6-based DNA-RNA immunoprecipitation and high-throughput sequencing. PMID: 31053798
RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage. PMID: 29742442
The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences. PMID: 28594954
Structural basis of R-loop recognition by the S9.6 monoclonal antibody. PMID: 35347133
Best practices for the visualization, mapping, and manipulation of R-loops. PMID: 33411340
Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging. PMID: 34232287
The Affinity of the S9.6 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast. PMID: 29289567
The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S9.6. PMID: 23784994
Modifications and interactions at the R-loop. PMID: 32961406
BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. PMID: 24896180
R-Loop Analysis by Dot-Blot. PMID: 33554969
Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead. PMID: 34324345
New insight into the biology of R-loops. PMID: 32516653
Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR. PMID: 33371452
Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation. PMID: 32105733
Complexities due to single-stranded RNA during antibody detection of genomic rna:dna hybrids. PMID: 25890199
Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening. PMID: 34495394
Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast. PMID: 29177737
Strengths and Weaknesses of the Current Strategies to Map and Characterize R-Loops. PMID: 29657305
Characterization of R-Loop Structures Using Single-Molecule R-Loop Footprinting and Sequencing. PMID: 32681515
Multi-marker diagnosis method for early Hepatocellular Carcinoma based on surface plasmon resonance. PMID: 31837298
Detection of DNA-RNA Hybrids In Vivo. PMID: 29043635
Simultaneous and ultrasensitive detection of multiple microRNAs by single-molecule fluorescence imaging. PMID: 34122849
Dissecting the Role of DDX21 in Regulating Human Cytomegalovirus Replication. PMID: 31554690
R-ChIP for genome-wide mapping of R-loops by using catalytically inactive RNASEH1. PMID: 30996261
Identification of R-loop-forming Sequences in Drosophila melanogaster Embryos and Tissue Culture Cells Using DRIP-seq. PMID: 34124311
Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells. PMID: 32258141
Effects of Benzophenone-3 and Propylparaben on Estrogen Receptor-Dependent R-Loops and DNA Damage in Breast Epithelial Cells and Mice. PMID: 31939680
Photoelectrochemical biosensor for microRNA detection based on a MoS2/g-C3N4/black TiO2 heterojunction with Histostar@AuNPs for signal amplification. PMID: 30660928
An antibody-based microarray assay for small RNA detection. PMID: 16614443
Establishment of immunoassay for detecting HPV16 E6 and E7 RNA. PMID: 26333509
Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice. PMID: 30345028
Antibodies directed to RNA/DNA hybrids: an electrochemical immunosensor for microRNAs detection using graphene-composite electrodes. PMID: 23930580
Topoisomerases I and III inhibit R-loop formation to prevent unregulated replication in the chromosomal Ter region of Escherichia coli. PMID: 30222737
Ribonucleoprotein-masked nicks at 50-kbp intervals in the eukaryotic genomic DNA. PMID: 17848525
RNA polymerase III regulates cytosolic RNA:DNA hybrids and intracellular microRNA expression. PMID: 25623070
Wang, P., Wei, X., Shen, L., Xu, K., Wen, Z., Gao, N., Fan, T., Xun, S., Zhu, Q., and Qu, X. (2024b). Amplification-Free Analysis of Bladder Cancer MicroRNAs on Wrinkled Silica Nanoparticles with DNA-Functionalized Quantum Dots. Analytical Chemistry 96, 4860-4867. PMID: 38478499
Liu, A., Wang, Y., Zheng, S., Bao, Z., Zhu, H., Yin, L., Liu, C., Zhao, X., Zhao, Z., Zhu, D., et al. (2024). Endonuclear Circ-calm4 Regulates Ferroptosis via a circR-Loop of the COMP Gene in Pulmonary Artery Smooth Muscle Cells. European Journal of Pharmacology, 176944. PMID: 39187041
For research use only. Not for human or drug use.
