Buy Anti-DNA-RNA Hybrid Antibody(S9.6) (mouse, IgG2a, kappa) for CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IF, IP, SPR

Catalog No.

RGK60001

Description

DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.

Expression system

Mammalian Cells

Species reactivity

General

Host species

mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Applications

CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IF, IP, SPR

Target

DNA-RNA Hybrid

Concentration

3.82 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Form

Liquid

Storage buffer

100 mM Pro-Ac, 20 mM Arg, pH 5.0

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

S9.6

Note

For research use only.

SDS-PAGE

SDS PAGE for DNA-RNA Hybrid Antibody(S9.6)
Conjugation

(a) SEM image and size distribution plot of WSNs. The scale bar is 200 nm. (b) TEM image of WSNs. The scale bar is 200nm. (c) ζ-Potential assay of surface modification of WSNs. (d) UV−vis absorption spectra of WSNs, Ab, and WSNs@Ab.
Data sourced from citation (PMID: 38478499)
Immunofluorescence

Figure 5. RNASEH1-R-loop signaling mediates PVC NPs-induced STING pathway activation in macrophages. A) Representative composite fluorescence images showing the levels of R-loops after treatment with different concentrations of PVC NPs. The R-loop and nuclei were labeled with an anti-DNA: RNA hybrid antibody (S9.6) (green) and DAPI (blue), respectively (scale bar = 5 μm). B) Quantitative analysis of the relative fluorescence intensity of R-loops in the nucleus of cells treated with different concentrations of PVC NPs (n > 50 cells). F) Representative R-loop PLA fluorescence images demonstrating the RNASEH1-R-loop interaction (scale bar = 5 μm). G) Quantitative analysis of PLA foci in the nuclear compartment (n > 50 cells). H) Representative composite fluorescence images showing the levels of R-loops after RNASEH1 overexpression following PVC NPs treatment (scale bar = 3 μm).
Data sourced from citation (PMID: 40919700)
Western Blot

Western blot analysis for RNA:DNA hybrid accumulation and DNA damage in 54 hpf embryos. A S9.6 antibody detected higher RNA:DNA hybrid levels in prpf4−/− embryos than in siblings.
Data sourced from citation (PMID: 41360772)
Bioactivity

(b) Fluorescence emission spectra of bQDs, bQDs-ssDNA1, and WSNs@Ab/bQDs-ssDNA1/miR-135b.
Data sourced from citation (PMID: 38478499)
Bioactivity

Optimization reaction conditions. (a)WSNs to S9.6 Ab mass ratio, (b) BSA concentration, and (c) QDs-ssDNA amount. As shown in Figure 4a, the fluorescence intensity increased with the ratios of WSNs to S9.6 Ab at 1:1,1:4, 1:7, and 1:10 and tended to be constant or even decreased along with the ratios more than 1:13 and 1:16. This indicated that Ab modified on the surface of WSNs reached saturation when the ratio was 1:10. The subsequent decrease may be due to the dense distribution of Ab causing steric hindrance to the capture of DNA/miRNA hybrids. To reduce the nonspecific adsorption of WSNs@Ab, we blocked the superficial active sites with a BSA-PBS solution. As shown in Figure 4b, the signal-to-background fluorescence ratio (F/F0, where F and F0 were the fluorescence intensities from the samples with and without miRNA, respectively) increased with BSA concentration and gained the highest signal at 0.25%. Insufficient concentration of BSA caused significant nonspecific adsorption of WSNs@Ab. Conversely, an excess concentration of BSA would adversely impact the Ab capture of hybrids. Moreover, the amount of QDs-ssDNA influenced the performance of miRNA detection. As shown in Figure 4c, the optimal amount was considered as 20 μL, as the fluorescence intensity reached a plateau at this volume. Thus, the mass ratio of WSNs to S9.6 Ab was set at 1:10, and 0.25% BSA and 20 μL of QDs-ssDNA were chosen for subsequent experiments.
Data sourced from citation (PMID: 38478499)

Prpf4 sequentially regulates the expansion and maturation of erythrocyte through distinct mechanisms. [RGK60001]

PVC Nanoplastics Exposure Exacerbates Asthma through R-Loop Accumulation and Subsequent STING Activation in Macrophages. [RGK60001]

Molecular Imaging of Cardiovascular Device Infection: Targeting the Bacteria or the Host-Pathogen Immune Response?, PMID:32034110

A sensitive fluorometric bio-barcodes immunoassay for detection of triazophos residue in agricultural products and water samples by iterative cycles of DNA-RNA hybridization and dissociation of fluorophores by Ribonuclease H., PMID:32084695

Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation., PMID:32105733

Simultaneous and ultrasensitive detection of multiple microRNAs by single-molecule fluorescence imaging., PMID:34122849

Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells., PMID:32258141

Development of a Versatile and Modular Linker for Antibody-Drug Conjugates Based on Oligonucleotide Strand Pairing., PMID:32526138

Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody., PMID:32681514

Characterization of R-Loop Structures Using Single-Molecule R-Loop Footprinting and Sequencing., PMID:32681515

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing., PMID:32756410

Design of a rapid, multiplex, one-pot miRNA assay optimized by label-free analysis., PMID:33137609

Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA., PMID:33273714

Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR., PMID:33371452

Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids., PMID:33830170

DNA-RNA Hybrid (R-Loop): From a Unified Picture of the Mammalian Telomere to the Genome-Wide Profile., PMID:34205454

Sensitivity and Specificity of Human Papillomavirus (HPV) 16 Early Antigen Serology for HPV-Driven Oropharyngeal Cancer: A Systematic Literature Review and Meta-Analysis., PMID:34208476

Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening., PMID:34495394

High-Throughput Selection and Characterisation of Aptamers on Optical Next-Generation Sequencers., PMID:34502110

Development of Antibodies against HPV-6 and HPV-11 for the Study of Laryngeal Papilloma., PMID:34696453

Structural basis of R-loop recognition by the S9.6 monoclonal antibody., PMID:35347133

DNAzyme signal amplification based on Au@Ag core-shell nanorods for highly sensitive SERS sensing miRNA-21., PMID:35419693

Direct R-Loop Visualization on Genomic DNA by Native Automated Electron Microscopy., PMID:35704181

Identification of RNA-DNA Hybrids Associated with R-Loops at the IgH Switch Sequence in Activated B Cells., PMID:35704185

DNA-RNA Hybrids at Telomeres in Budding Yeast., PMID:35704190

Detection of TERRA R-Loops at Human Telomeres., PMID:35704191

Genome-Wide Native R-Loop Profiling by R-Loop Cleavage Under Targets and Tagmentation (R-Loop CUT&Tag)., PMID:35704203

Quantitative DNA-RNA Immunoprecipitation Sequencing with Spike-Ins., PMID:35704206

DNA:RNA Immunoprecipitation from S. pombe Cells for qPCR and Genome-Wide Sequencing., PMID:35704207

Genome-Wide Analysis of DNA-RNA Hybrids in Yeast by DRIPc-Seq and DRIP-Seq., PMID:35704208

Fully automated microRNA quantification technique based on bioluminescent enzyme immunoassay., PMID:36063916

A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching., PMID:36105157

Janus USPION modular platform (JUMP) for theranostic ultrasound-mediated targeted intratumoral microvascular imaging and DNA/miRNA delivery., PMID:36451861

First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential "hidden" food allergen., PMID:36571998

A rapid and simple method for simultaneous determination of three breast cancer related microRNAs based on magnetic nanoparticles modified with S9.6 antibody., PMID:36682580

Primer exchange reaction-amplified protein-nucleic acid interactions for ultrasensitive and specific microRNA detection., PMID:37004284

Electrochemical bioplatform for the determination of the most common and carcinogenic human papillomavirus DNA., PMID:37094410

Detecting R-Loop Formation Using a Plasmid-Based In Vitro Transcription Assay., PMID:37166671

S9.6 Antibody-Enzyme Conjugates for the Detection of DNA-RNA Hybrids., PMID:37194248

Role of Myeloperoxidase in ROS Generation and Inflammation Response on Prostate Epithelial Cells., PMID:37318620

Slot Blot Assay for Detection of R Loops., PMID:37574480

Strategies and Applications of Graphene and Its Derivatives-Based Electrochemical Sensors in Cancer Diagnosis., PMID:37764496

S9.6-based hybrid capture immunoassay for pathogen detection., PMID:38110611

RNA helicase DEAD-box-5 is involved in R-loop dynamics of preimplantation embryos., PMID:38419548

Approaches for Mapping and Analysis of R-loops., PMID:38666626

Smartphone-based device for rapid and single-step detection of piRNA-651 using anti-DNA:RNA hybrid antibody and enzymatic signal amplification., PMID:38677845

RNA Analysis Using Immunoassay Detection Format., PMID:38907919

Image Analysis Protocol for DNA/RNA/Immunofluorescence (IF)-seqFISH Data., PMID:39283466

Immuno-transcription-amplified single microbead assay for protein and exosome analysis through an S9.6 antibody-nucleic acid recognition strategy., PMID:39657553

ARNAX is an ideal adjuvant for COVID-19 vaccines to enhance antigen-specific CD4+ and CD8+ T-cell responses and neutralizing antibody induction., PMID:40231823

S9.6 Antibody-Mediated Bivalent Capture Biosensor Using Vacuum-Assembled HCR Amplifier for One-Pot, Record-Fast and Ultrasensitive Detection of Nucleic Acid., PMID:40317267

An immunoassay based on polyHRP signal amplification for HPV16/18 E6 RNA detection with anti-DNA-RNA hybrid antibody., PMID:40531470

Amplification-Free Analysis of Bladder Cancer MicroRNAs on Wrinkled Silica Nanoparticles with DNA-Functionalized Quantum Dots. [RGK60001]

Endonuclear Circ-calm4 Regulates Ferroptosis via a circR-Loop of the COMP Gene in Pulmonary Artery Smooth Muscle Cells. [RGK60001]

RNA polymerase III regulates cytosolic RNA:DNA hybrids and intracellular microRNA expression. PMID: 25623070

Ribonucleoprotein-masked nicks at 50-kbp intervals in the eukaryotic genomic DNA. PMID: 17848525

Topoisomerases I and III inhibit R-loop formation to prevent unregulated replication in the chromosomal Ter region of Escherichia coli. PMID: 30222737

Antibodies directed to RNA/DNA hybrids: an electrochemical immunosensor for microRNAs detection using graphene-composite electrodes. PMID: 23930580

Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice. PMID: 30345028

Establishment of immunoassay for detecting HPV16 E6 and E7 RNA. PMID: 26333509

An antibody-based microarray assay for small RNA detection. PMID: 16614443

Photoelectrochemical biosensor for microRNA detection based on a MoS2/g-C3N4/black TiO2 heterojunction with Histostar@AuNPs for signal amplification. PMID: 30660928

Effects of Benzophenone-3 and Propylparaben on Estrogen Receptor-Dependent R-Loops and DNA Damage in Breast Epithelial Cells and Mice. PMID: 31939680

Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells. PMID: 32258141

Identification of R-loop-forming Sequences in Drosophila melanogaster Embryos and Tissue Culture Cells Using DRIP-seq. PMID: 34124311

R-ChIP for genome-wide mapping of R-loops by using catalytically inactive RNASEH1. PMID: 30996261

Dissecting the Role of DDX21 in Regulating Human Cytomegalovirus Replication. PMID: 31554690

Simultaneous and ultrasensitive detection of multiple microRNAs by single-molecule fluorescence imaging. PMID: 34122849

Detection of DNA-RNA Hybrids In Vivo. PMID: 29043635

Multi-marker diagnosis method for early Hepatocellular Carcinoma based on surface plasmon resonance. PMID: 31837298

Characterization of R-Loop Structures Using Single-Molecule R-Loop Footprinting and Sequencing. PMID: 32681515

Strengths and Weaknesses of the Current Strategies to Map and Characterize R-Loops. PMID: 29657305

Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast. PMID: 29177737

Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening. PMID: 34495394

Complexities due to single-stranded RNA during antibody detection of genomic rna:dna hybrids. PMID: 25890199

Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation. PMID: 32105733

Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR. PMID: 33371452

New insight into the biology of R-loops. PMID: 32516653

Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead. PMID: 34324345

R-Loop Analysis by Dot-Blot. PMID: 33554969

BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. PMID: 24896180

Modifications and interactions at the R-loop. PMID: 32961406

The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S9.6. PMID: 23784994

The Affinity of the S9.6 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast. PMID: 29289567

Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging. PMID: 34232287

Best practices for the visualization, mapping, and manipulation of R-loops. PMID: 33411340

Structural basis of R-loop recognition by the S9.6 monoclonal antibody. PMID: 35347133

The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences. PMID: 28594954

RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage. PMID: 29742442

High-resolution, strand-specific R-loop mapping via S9.6-based DNA-RNA immunoprecipitation and high-throughput sequencing. PMID: 31053798

Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody. PMID: 32681514

Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids. PMID: 33830170

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Anti-DNA-RNA Hybrid Antibody (S9.6).pdf

Anti-DNA-RNA Hybrid Antibody (S9.6)