RAW264.7 whole cell lysate

Catalog No.

CLM00030

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Rac1 inhibition protects the kidney against kidney ischemia/reperfusion through the inhibition of macrophage migration., PMID:37078299

Blood Clots versus PRF: Activating TGF-β Signaling and Inhibiting Inflammation In Vitro., PMID:35682575

Euodia pasteuriana Methanol Extract Exerts Anti-Inflammatory Effects by Targeting TAK1 in the AP-1 Signaling Pathway., PMID:33297427

Whole Genome Analysis of Lactobacillus plantarum Strains Isolated From Kimchi and Determination of Probiotic Properties to Treat Mucosal Infections by Candida albicans and Gardnerella vaginalis., PMID:30894844

Suppression of Src and Syk in the NF-κB signaling pathway by Olea europaea methanol extract is leading to its anti-inflammatory effects., PMID:30710734

Momordica charantia Inhibits Inflammatory Responses in Murine Macrophages via Suppression of TAK1., PMID:29463104

Pyrrole-Derivative of Chalcone, (E)-3-Phenyl-1-(2-Pyrrolyl)-2-Propenone, Inhibits Inflammatory Responses via Inhibition of Src, Syk, and TAK1 Kinase Activities., PMID:27469142

Identification of heme oxygenase-1 stimulators by a convenient ELISA-based bilirubin quantification assay., PMID:25462643

In vitro and in vivo anti-inflammatory effect of Rhodomyrtus tomentosa methanol extract., PMID:23295168

Src and Syk are targeted to an anti-inflammatory ethanol extract of Aralia continentalis., PMID:22885130

Inhibition of prostaglandin E2 synthesis by SC-560 is independent of cyclooxygenase 1 inhibition., PMID:16816110

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RAW264.7 whole cell lysate.pdf