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NIH/3T3 whole cell lysate

Catalog #:   CLM00026 Specific References (50) DATASHEET
Applications: WB
Overview

Catalog No.

CLM00026

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Data Image
References

An in vivo "turning model" reveals new RanBP9 interactions in lung macrophages., PMID:40223093

A method for the detection and enrichment of endogenous cereblon substrates., PMID:40196695

A novel, rapidly progressive ataxia due to a spontaneous Myo5a mutation in mice impairs transport proteins and alters mitochondria., PMID:40022605

GTPBP2 in-frame deletion in canine model with non-syndromic progressive retinal atrophy., PMID:39971978

Theranostic Near-Infrared Monoamine Oxidase Inhibitor (NMI) Protein Binding Interactions with MAOA and Albumin., PMID:39904854

Blocking autophagosome closure manifests the roles of mammalian Atg8-family proteins in phagophore formation and expansion during nutrient starvation., PMID:39694607

Ultradeep O-GlcNAc proteomics reveals widespread O-GlcNAcylation on tyrosine residues of proteins., PMID:39531497

Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays., PMID:39526730

Pancreatic β-cells package double C2-like domain beta protein into extracellular vesicles via tandem C2 domains., PMID:39497805

A rapid, facile, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies., PMID:39484553

Replication and extension of the subregion selectivity of glutamate-related changes within the nucleus accumbens associated with the incubation of cocaine-craving., PMID:39389205

Intestinal Epithelial PTPN2 Limits Pathobiont Colonization by Immune-Directed Antimicrobial Responses., PMID:39386684

Endothelial Neurogranin Regulates Blood-Brain Barrier Permeability via Modulation of the AKT Pathway., PMID:39367201

Update on a brain-penetrant cardiac glycoside that can lower cellular prion protein levels in human and guinea pig paradigms., PMID:39316592

Native Proteomics by Capillary Zone Electrophoresis-Mass Spectrometry., PMID:39196601

GSK3β Inhibitors Inhibit TGFβ Signaling in the Human Trabecular Meshwork., PMID:39087933

Radiation-induced eCIRP impairs macrophage bacterial phagocytosis., PMID:38920274

One-pot method for preparing DNA, RNA, and protein for multiomics analysis., PMID:38485785

Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays., PMID:38328090

Engineering Challenges and Opportunities in Autologous Cellular Cancer Immunotherapy., PMID:38166251

Tubular deficiency of ABCA1 augments cholesterol- and Na+-dependent effects on systemic blood pressure in male mice., PMID:38153852

The murine metastatic microenvironment of experimental brain metastases of breast cancer differs by host age in vivo: a proteomic study., PMID:37917186

Protocol for quantifying the in vivo rate of protein degradation in mice using a pulse-chase technique., PMID:37729055

All-in-One Pseudo-MS3 Method for the Analysis of Gas-Phase Cleavable Protein Crosslinking Reactions., PMID:37590165

Red blood cell exposure increases chondrocyte susceptibility to oxidative stress following hemarthrosis., PMID:37364817

Engineered tumor-specific T cells using immunostimulatory photothermal nanoparticles., PMID:37278683

Improved Label-Free Quantification of Intact Proteoforms Using Field Asymmetric Ion Mobility Spectrometry., PMID:37252723

Quantitative Proteomics Reveals Transforming Growth Factor β Receptor Targeted by Resveratrol and Hesperetin Coformulation in Endothelial Cells., PMID:37179642

Pan-cancer analysis identifies tumor-specific antigens derived from transposable elements., PMID:36973455

A20 Restricts NOS2 Expression and Intestinal Tumorigenesis in a Mouse Model of Colitis-Associated Cancer., PMID:36636264

Ang II acutely stimulates Na,K-pump in cells from proximal tubules by increasing its phosphorylation at S938 via a PI3K/AKT pathway., PMID:36377055

Miniprep assisted proteomics (MAP) for rapid proteomics sample preparation., PMID:36373982

AMPK-driven Macrophage Responses Are Autophagy Dependent in Experimental Bronchopulmonary Dysplasia., PMID:36306501

2-Difluoromethoxy-Substituted Estratriene Sulfamates: Synthesis, Antiproliferative SAR, Antitubulin Activity, and Steroid Sulfatase Inhibition., PMID:36109340

The Transcriptional Regulator SpxA1 Influences the Morphology and Virulence of Listeria monocytogenes., PMID:36102657

New, potent, small molecule agonists of tyrosine kinase receptors attenuate dry eye disease., PMID:36091713

Treatment of Sindbis Virus-Infected Neurons with Antibody to E2 Alters Synthesis of Complete and nsP1-Expressing Defective Viral RNAs., PMID:36069441

Lysate and cell-based assays to probe the translational role of RNA helicases., PMID:35965005

Protection Induced by Oral Vaccination with a Recombinant Yersinia pseudotuberculosis Delivering Yersinia pestis LcrV and F1 Antigens in Mice and Rats against Pneumonic Plague., PMID:35900096

The Role of Glutathione Reductase in Influenza Infection., PMID:35767671

A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader., PMID:35649410

Quantification of tissue-specific protein translation in whole C. elegans using O-propargyl-puromycin labeling and fluorescence microscopy., PMID:35497499

AMPK Signaling Regulates Mitophagy and Mitochondrial ATP Production in Human Trophoblast Cell Line BeWo., PMID:35468677

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis., PMID:35435918

REL and BHLHE40 Variants Are Associated with IL-12 and IL-10 Responses and Tuberculosis Risk., PMID:35217585

Exploiting flow cytometry for the unbiased quantification of protein inclusions in Caenorhabditis elegans., PMID:35170035

PKA and Epac1 Reduce Nek7 to Block the NLRP3 Inflammasome Proteins in the Retinal Vasculature., PMID:35006270

A novel assay to assess the effects of estrogen on the cardiac calmodulin binding equilibrium., PMID:34954214

Reanalysis of Exome Data Identifies Novel SLC25A46 Variants Associated with Leigh Syndrome., PMID:34945750

Preclinical evidence for pioglitazone and bexarotene combination in oral cancer chemoprevention., PMID:34931381

Datasheet

Document Download

NIH/3T3 whole cell lysate.pdf

 

$ 167
Product specifications
100 μg 167

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For research use only. Not for human or drug use.

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NIH/3T3 whole cell lysate [CLM00026]
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