Catalog No.
CLH00082
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Engineering Challenges and Opportunities in Autologous Cellular Cancer Immunotherapy., PMID:38166251
Decitabine, a DNA methyltransferases inhibitor, induces cell cycle arrest at G2/M phase through p53-independent pathway in human cancer cells., PMID:23582784
In vitro cytokine profiles and viability of different human cells treated with whole cell lysate of Mycobacterium avium subsp. paratuberculosis., PMID:23006537
Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer., PMID:22204653
Targeting PI3K/Akt/HSP90 signaling sensitizes gastric cancer cells to deoxycholate-induced apoptosis., PMID:20585984
Characterization of immunogenic proteins of Trypanosoma evansi isolated from three different Indian hosts using hyperimmune sera and immune sera., PMID:18371998
Naturally occurring IgM anti-leukocyte autoantibodies (IgM-ALA) inhibit T cell activation and chemotaxis., PMID:18209075
Polymer nanoparticles for immunotherapy from encapsulated tumor-associated antigens and whole tumor cells., PMID:17217312
Preparation of purified lymphoma cells suitable for therapy., PMID:15203980
Immunochemical characterisation of Vibrio cholerae O139 O antigens and production of a diagnostic antiserum without absorption., PMID:11393287
For research use only. Not for human or drug use.
