Catalog No.
RGK11201
Species reactivity
General
Host species
Mouse
Isotype
IgG1, kappa
Clonality
Monoclonal
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:5000
Target
NeuGcGM3, Ganglioside N-glycolyl GM3, N-glycolylneuraminic acid -NeuGc, NGNA-GM3 ganglioside, NeuGcGM3
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
14F7
14F7 Anti-GM3(NeuGc)ganglioside. PMID: 10954880
Key role of a structural water molecule for the specificity of 14F7-An antitumor antibody targeting the NeuGc GM3 ganglioside. PMID: 34735569
Immunoreactivity of the 14F7 Mab raised against N-Glycolyl GM3 Ganglioside in retinoblastoma tumours. PMID: 25403557
Prognostic Role of 14F7 Mab Immunoreactivity against N-Glycolyl GM3 Ganglioside in Colon Cancer. PMID: 24639871
Recombinant AAV-mediated in vivo long-term expression and antitumour activity of an anti-ganglioside GM3(Neu5Gc) antibody. PMID: 26181624
Tissue Reactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Tumors of Neuroectodermal, Mesodermal, and Epithelial Origin. PMID: 26317019
Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis. PMID: 24381785
Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Epithelial Malignant Tumors from Digestive System. PMID: 21991524
Immunohistochemical Reactivity of the 14F7 Monoclonal Antibody Raised against N-Glycolyl GM3 Ganglioside in Some Benign and Malignant Skin Neoplasms. PMID: 22363862
Immunoreactivity of the 14F7 Mab (Raised against N-Glycolyl GM3 Ganglioside) as a Positive Prognostic Factor in Non-Small-Cell Lung Cancer. PMID: 22482082
Structure and molecular interactions of a unique antitumor antibody specific for N-glycolyl GM3. PMID: 14627696
Crystal structure of an L chain optimised 14F7 anti-ganglioside Fv suggests a unique tumour-specificity through an unusual H-chain CDR3 architecture. PMID: 30022069
Clinical evidences of GM3 (NeuGc) ganglioside expression in human breast cancer using the 14F7 monoclonal antibody labelled with (99m)Tc. PMID: 16322892
Engineering the binding site of an antibody against N-glycolyl GM3: from functional mapping to novel anti-ganglioside specificities. PMID: 23138862
A shift from N-glycolyl- to N-acetyl-sialic acid in the GM3 ganglioside impairs tumor development in mouse lymphocytic leukemia cells. PMID: 23547010
Insights into the immunogenetic basis of two ganglioside-associated idiotypic networks. PMID: 17270710
In vivo and in vitro anti-tumor effect of 14F7 monoclonal antibody. PMID: 12573110
Generation and characterization of an anti-idiotype monoclonal antibody related to GM3(NeuGc) ganglioside. PMID: 14678648
Prognostic Significance of N-Glycolyl GM3 Ganglioside Expression in Non-Small Cell Lung Carcinoma Patients: New Evidences. PMID: 26634172
Light-chain shuffling results in successful phage display selection of functional prokaryotic-expressed antibody fragments to N-glycolyl GM3 ganglioside. PMID: 15541278
A cytotoxic humanized anti-ganglioside antibody produced in a murine cell line defective of N-glycolylated-glycoconjugates. PMID: 21802167
Detection of N-glycolyl GM3 ganglioside in neuroectodermal tumors by immunohistochemistry: an attractive vaccine target for aggressive pediatric cancer. PMID: 21941577
A mouse IgG1 monoclonal antibody specific for N-glycolyl GM3 ganglioside recognized breast and melanoma tumors. PMID: 10952412
Cytofluorimetric evaluation of N-glycolylated GM3 ganglioside expression on murine leukocytes. PMID: 21324343
Aberrant expression of N-glycolyl GM3 ganglioside is associated with the aggressive biological behavior of human sarcomas. PMID: 31182063
N-Glycolyl GM3 ganglioside immunoexpression in oral mucosal melanomas of Chinese. PMID: 22574836
Switching on cytotoxicity by a single mutation at the heavy chain variable region of an anti-ganglioside antibody. PMID: 21306777
Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry. PMID: 19435393
Hypothesis: Hypoxia induces de novo synthesis of NeuGc gangliosides in humans through CMAH domain substitute. PMID: 29196263
Exogenous incorporation of neugc-rich mucin augments n-glycolyl sialic acid content and promotes malignant phenotype in mouse tumor cell lines. PMID: 19951433
CAR T cells targeting the ganglioside NGcGM3 control ovarian tumors in the absence of toxicity against healthy tissues. PMID: 35990626
Synergistic potentiation of the anti-metastatic effect of anti EGFR mAb by its combination with immunotherapies targeting the ganglioside NGcGM3. PMID: 29844873
For research use only. Not for human or drug use.
