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3C Protease

Catalog #:   YVV07701 Specific References (50) DATASHEET
Applications: Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Expression system: E.coli
Protein length: 3C Protease is cloned from Human rhinovirus and expressed in E.coli.
Overview

Catalog No.

YVV07701

Description

3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.

Biological activity

One unit will cleave ≥95% of 100μg of GST-tagged protein in 16 hours at 4°C.

Expression system

E.coli

Species

HRV-14

Protein length

3C Protease is cloned from Human rhinovirus and expressed in E.coli.

Predicted molecular weight

47.46 kDa

Nature

Recombinant

Target

3C Protease

Concentration

500U/mL

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Applications

Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).

Form

Liquid

Storage buffer

50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Data Image
  • SDS-PAGE
    SDS PAGE for recombinant HRV-14 3C Protease
  • Experiment Example
    The control protein was cleaved by 3C protease at 4°C for 16 h.
References

A novel cellular tool for screening human pan-coronavirus antivirals., PMID:40505777

3C protease-independent production of foot-and-mouth disease virus-like particles in Pichia pastoris., PMID:40504260

GSDMD and GSDME exhibit distinct roles in enteric coronavirus PDCoV-induced pyroptosis and inflammatory responses., PMID:40503916

Strategy to overcome a nirmatrelvir resistance mechanism in the SARS-CoV-2 nsp5 protease., PMID:40479048

Trim7 does not have a role in the restriction of murine norovirus infection in vivo., PMID:40464581

Discovery of non-covalent rhinovirus 3Cpro inhibitors by molecular docking, in vitro assays, molecular dynamics simulations and DFT analyses., PMID:40453662

Influence of Steric and Electronic Properties of P2 Groups on Covalent Inhibitor Binding to SARS-CoV-2 Main Protease., PMID:40437897

Drug repurposing targeting COVID-19 3CL protease using molecular docking and machine learning regression approaches., PMID:40436944

Exploration of structure-activity relationships for the SARS-CoV-2 macrodomain from shape-based fragment linking and active learning., PMID:40435250

Molecular Recognition of SARS-CoV-2 Mpro Inhibitors: Insights from Cheminformatics and Quantum Chemistry., PMID:40430346

The Effects of Ferulic Acid on the Growth Performance, Immune Function, Antioxidant Capacity, and Intestinal Microbiota of Broiler Chickens., PMID:40428394

COVID-19 clinical outcomes and N-acetylcysteine (CoViNAC study): a GRADE compliant meta-analysis of randomized controlled trials with molecular docking and dynamics simulation studies with Mpro of SARS-CoV-2., PMID:40418545

Peptidomimetic Phenoxymethyl Ketone Warheads as Potent Dual-Mode Inhibitors against SARS-CoV-2 Mpro and Cathepsin., PMID:40415551

Ursane hybrids with 5-amino-1,2,3,4-thiatriazole, 1-tetrazole-5-thione, and 1-tetrazole-5-amines and study of their inhibition of main SARS-CoV-2 protease., PMID:40409429

The Challenge of Type 3c Diabetes: From Accurate Diagnosis to Effective Treatment., PMID:40401174

Cullin 3-mediated ubiquitination restricts enterovirus D68 replication and is counteracted by viral protease 3C., PMID:40396757

Identification of broad-spectrum Mpro inhibitors: a focus on high-risk coronaviruses and conserved interactions., PMID:40396609

Design and Evaluation of Andrographolide Analogues as SARS-CoV-2 Main Protease Inhibitors: Molecular Modeling and in vitro Studies., PMID:40391180

Computational and Experimental Study of the Conformational Variation of the Catalytic Residue His41 of the SARS-CoV-2 Main Protease., PMID:40387138

Identification of naturally occurring drug-resistant mutations of SARS-CoV-2 papain-like protease., PMID:40379662

A novel, covalent broad-spectrum inhibitor targeting human coronavirus Mpro., PMID:40374668

Discovery of baicalein derivatives as novel covalent inhibitors of SARS CoV-2 Mpro: Structure-activity relationships and inhibitory mechanisms., PMID:40367796

Virtual Screening of Phytoconstituents in Indian Spices Based on their Inhibitory Potential against SARS-CoV-2., PMID:40353411

[Oxocrebanine inhibits proliferation of hepatoma HepG2 cells by inducing apoptosis and autophagy]., PMID:40350949

Thiol esters as chemical warheads of SARS-CoV-2 main protease (3CLpro) peptide-like inhibitors., PMID:40344734

Gene Therapy with Enterovirus 3 C Protease: A Promising Strategy for Various Solid Tumors., PMID:40340965

Development of Peptidomimetic PROTACs as Potential Degraders of 3-Chymotrypsin-like Protease of SARS-CoV-2., PMID:40332755

Peptides designed based on 3C substrates exhibit antiviral efficacy in vivo., PMID:40318791

Multi-immobilization of viscozyme, alcalase and flavourzyme by nanomagnetic combi-CLEAs method for oil and protein hydrolysates extraction from rice bran in aqueous phase., PMID:40316118

Development of a magnetic beads-based ligand fishing method for screening SARS-CoV-2 NSP5 inhibitors from complex herbal mixtures: Rosmarinus officinalis as a case study., PMID:40300453

Foot-and-mouth disease virus 3C protein acts as an anti-inflammatory factor by mediating degradation of TLR4 signaling various molecules via caspase activity., PMID:40300413

Ensitrelvir as a novel treatment option for mild-to-moderate COVID-19: a narrative literature review., PMID:40292087

Designing highly efficient heterocyclic inhibitors for SARS-CoV-2 3C-like proteinase: a comprehensive in silico study., PMID:40287959

The Dynamical Asymmetry in SARS-CoV2 Protease Reveals the Exchange Between Catalytic Activity and Stability in Homodimers., PMID:40286026

In Silico and In Vitro Studies of the Approved Antibiotic Ceftaroline Fosamil and Its Metabolites as Inhibitors of SARS-CoV-2 Replication., PMID:40284934

5,6-dihydroxyflavone exerts anti-betacoronavirus activity by blocking viral entry to host cells., PMID:40280493

Structure-based discovery of highly bioavailable, covalent, broad-spectrum coronavirus MPro inhibitors with potent in vivo efficacy., PMID:40267184

Identification of potential SARS-CoV-2 inhibitors among well-tolerated drugs using drug repurposing and in vitro approaches., PMID:40263343

Elucidating the structural dynamics induced by active site mutations in 3C protease of foot-and-mouth disease virus., PMID:40257971

Discovery of cinnamoyl-flavonoid hybrid derivatives as inhibitors of SARS-CoV-2 Mpro and anti-inflammatory agents: Experimental and in silico insights into their efficacy against lipopolysaccharide-induced lung injury., PMID:40252899

Ensitrelvir in Hospitalized Patients with SARS-CoV-2 During the Omicron Epidemic: A Single-Center Observational Study., PMID:40252170

Machine Learning-Guided Screening and Molecular Docking for Proposing Naturally Derived Drug Candidates Against MERS-CoV 3CL Protease., PMID:40243651

A deep learning model for structure-based bioactivity optimization and its application in the bioactivity optimization of a SARS-CoV-2 main protease inhibitor., PMID:40239482

A phycobiliprotein-based reporter assay for the evaluation of SARS-CoV-2 main protease activity., PMID:40233445

Enhancing the understandings on SARS-CoV-2 main protease (Mpro) mutants from molecular dynamics and machine learning., PMID:40220823

Silaproline-bearing nirmatrelvir derivatives are potent inhibitors of the SARS-CoV-2 main protease highlighting the value of silicon-derivatives in structure-activity-relationship studies., PMID:40220677

Synthesis, TD-DFT calculations, molecular docking and ADME studies of new spiro-oxindole derivatives containing 5(4H)-oxazolone as anti-viral and anti-bacterial agents., PMID:40214849

Hyaluronic acid modified chuanxiong oil liposomes as a novel therapeutic agent for photoaging prevention., PMID:40210731

Synthesis of new selective agents with dual anti-inflammatory and SARS-CoV-2 Mpro inhibitory activity: Antipyrine-celecoxib hybrid analogues; COX-2, COVID-19 cytokine storm and replication inhibitory activities., PMID:40199011

Targeting Viperin prevents coxsackievirus B3-induced acute heart failure., PMID:40195316

Datasheet

Document Download

3C Protease.pdf

 

$ 300
Product specifications
1,000 U 300 5,000 U 1200

Contact Information

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For research use only. Not for human or drug use.

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3C Protease [YVV07701]
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