Catalog No.
YVV07701
Description
3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Biological activity
One unit will cleave ≥95% of 100μg of GST-tagged protein in 16 hours at 4°C.
Expression system
E.coli
Species
HRV-14
Protein length
3C Protease is cloned from Human rhinovirus and expressed in E.coli.
Predicted molecular weight
47.46 kDa
Nature
Recombinant
Target
3C Protease
Concentration
500U/mL
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Applications
Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Form
Liquid
Storage buffer
50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
For research use only. Not for human or drug use.
