Catalog No.
YVV07701
Description
3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Biological activity
One unit will cleave ≥95% of 100μg of GST-tagged protein in 16 hours at 4°C.
Expression system
E.coli
Species
HRV-14
Protein length
3C Protease is cloned from Human rhinovirus and expressed in E.coli.
Predicted molecular weight
47.46 kDa
Nature
Recombinant
Applications
Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Target
3C Protease
Concentration
500U/mL
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Form
Liquid
Storage buffer
50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Note
For research use only.
Targeting Viperin prevents coxsackievirus B3-induced acute heart failure., PMID:40195316
Synthesis of new selective agents with dual anti-inflammatory and SARS-CoV-2 Mpro inhibitory activity: Antipyrine-celecoxib hybrid analogues; COX-2, COVID-19 cytokine storm and replication inhibitory activities., PMID:40199011
Hyaluronic acid modified chuanxiong oil liposomes as a novel therapeutic agent for photoaging prevention., PMID:40210731
Synthesis, TD-DFT calculations, molecular docking and ADME studies of new spiro-oxindole derivatives containing 5(4H)-oxazolone as anti-viral and anti-bacterial agents., PMID:40214849
Silaproline-bearing nirmatrelvir derivatives are potent inhibitors of the SARS-CoV-2 main protease highlighting the value of silicon-derivatives in structure-activity-relationship studies., PMID:40220677
Enhancing the understandings on SARS-CoV-2 main protease (Mpro) mutants from molecular dynamics and machine learning., PMID:40220823
A phycobiliprotein-based reporter assay for the evaluation of SARS-CoV-2 main protease activity., PMID:40233445
A deep learning model for structure-based bioactivity optimization and its application in the bioactivity optimization of a SARS-CoV-2 main protease inhibitor., PMID:40239482
Machine Learning-Guided Screening and Molecular Docking for Proposing Naturally Derived Drug Candidates Against MERS-CoV 3CL Protease., PMID:40243651
Ensitrelvir in Hospitalized Patients with SARS-CoV-2 During the Omicron Epidemic: A Single-Center Observational Study., PMID:40252170
Discovery of cinnamoyl-flavonoid hybrid derivatives as inhibitors of SARS-CoV-2 Mpro and anti-inflammatory agents: Experimental and in silico insights into their efficacy against lipopolysaccharide-induced lung injury., PMID:40252899
Elucidating the structural dynamics induced by active site mutations in 3C protease of foot-and-mouth disease virus., PMID:40257971
Identification of potential SARS-CoV-2 inhibitors among well-tolerated drugs using drug repurposing and in vitro approaches., PMID:40263343
Structure-based discovery of highly bioavailable, covalent, broad-spectrum coronavirus MPro inhibitors with potent in vivo efficacy., PMID:40267184
5,6-dihydroxyflavone exerts anti-betacoronavirus activity by blocking viral entry to host cells., PMID:40280493
In Silico and In Vitro Studies of the Approved Antibiotic Ceftaroline Fosamil and Its Metabolites as Inhibitors of SARS-CoV-2 Replication., PMID:40284934
The Dynamical Asymmetry in SARS-CoV2 Protease Reveals the Exchange Between Catalytic Activity and Stability in Homodimers., PMID:40286026
Designing highly efficient heterocyclic inhibitors for SARS-CoV-2 3C-like proteinase: a comprehensive in silico study., PMID:40287959
Ensitrelvir as a novel treatment option for mild-to-moderate COVID-19: a narrative literature review., PMID:40292087
Foot-and-mouth disease virus 3C protein acts as an anti-inflammatory factor by mediating degradation of TLR4 signaling various molecules via caspase activity., PMID:40300413
Development of a magnetic beads-based ligand fishing method for screening SARS-CoV-2 NSP5 inhibitors from complex herbal mixtures: Rosmarinus officinalis as a case study., PMID:40300453
Multi-immobilization of viscozyme, alcalase and flavourzyme by nanomagnetic combi-CLEAs method for oil and protein hydrolysates extraction from rice bran in aqueous phase., PMID:40316118
Peptides designed based on 3C substrates exhibit antiviral efficacy in vivo., PMID:40318791
Development of Peptidomimetic PROTACs as Potential Degraders of 3-Chymotrypsin-like Protease of SARS-CoV-2., PMID:40332755
Gene Therapy with Enterovirus 3 C Protease: A Promising Strategy for Various Solid Tumors., PMID:40340965
Thiol esters as chemical warheads of SARS-CoV-2 main protease (3CLpro) peptide-like inhibitors., PMID:40344734
[Oxocrebanine inhibits proliferation of hepatoma HepG2 cells by inducing apoptosis and autophagy]., PMID:40350949
Virtual Screening of Phytoconstituents in Indian Spices Based on their Inhibitory Potential against SARS-CoV-2., PMID:40353411
Discovery of baicalein derivatives as novel covalent inhibitors of SARS CoV-2 Mpro: Structure-activity relationships and inhibitory mechanisms., PMID:40367796
A novel, covalent broad-spectrum inhibitor targeting human coronavirus Mpro., PMID:40374668
Identification of naturally occurring drug-resistant mutations of SARS-CoV-2 papain-like protease., PMID:40379662
Computational and Experimental Study of the Conformational Variation of the Catalytic Residue His41 of the SARS-CoV-2 Main Protease., PMID:40387138
Design and Evaluation of Andrographolide Analogues as SARS-CoV-2 Main Protease Inhibitors: Molecular Modeling and in vitro Studies., PMID:40391180
Identification of broad-spectrum Mpro inhibitors: a focus on high-risk coronaviruses and conserved interactions., PMID:40396609
Cullin 3-mediated ubiquitination restricts enterovirus D68 replication and is counteracted by viral protease 3C., PMID:40396757
The Challenge of Type 3c Diabetes: From Accurate Diagnosis to Effective Treatment., PMID:40401174
Ursane hybrids with 5-amino-1,2,3,4-thiatriazole, 1-tetrazole-5-thione, and 1-tetrazole-5-amines and study of their inhibition of main SARS-CoV-2 protease., PMID:40409429
Peptidomimetic Phenoxymethyl Ketone Warheads as Potent Dual-Mode Inhibitors against SARS-CoV-2 Mpro and Cathepsin., PMID:40415551
COVID-19 clinical outcomes and N-acetylcysteine (CoViNAC study): a GRADE compliant meta-analysis of randomized controlled trials with molecular docking and dynamics simulation studies with Mpro of SARS-CoV-2., PMID:40418545
The Effects of Ferulic Acid on the Growth Performance, Immune Function, Antioxidant Capacity, and Intestinal Microbiota of Broiler Chickens., PMID:40428394
Molecular Recognition of SARS-CoV-2 Mpro Inhibitors: Insights from Cheminformatics and Quantum Chemistry., PMID:40430346
Exploration of structure-activity relationships for the SARS-CoV-2 macrodomain from shape-based fragment linking and active learning., PMID:40435250
Drug repurposing targeting COVID-19 3CL protease using molecular docking and machine learning regression approaches., PMID:40436944
Influence of Steric and Electronic Properties of P2 Groups on Covalent Inhibitor Binding to SARS-CoV-2 Main Protease., PMID:40437897
Discovery of non-covalent rhinovirus 3Cpro inhibitors by molecular docking, in vitro assays, molecular dynamics simulations and DFT analyses., PMID:40453662
Trim7 does not have a role in the restriction of murine norovirus infection in vivo., PMID:40464581
Strategy to overcome a nirmatrelvir resistance mechanism in the SARS-CoV-2 nsp5 protease., PMID:40479048
GSDMD and GSDME exhibit distinct roles in enteric coronavirus PDCoV-induced pyroptosis and inflammatory responses., PMID:40503916
3C protease-independent production of foot-and-mouth disease virus-like particles in Pichia pastoris., PMID:40504260
A novel cellular tool for screening human pan-coronavirus antivirals., PMID:40505777
For research use only. Not for human or drug use.
