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Anti-Hypusine antibody (Hpu24) (RGK08101)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Expression system:Mammalian Cells
Overview

Catalog No.

RGK08101

Expression system

Mammalian Cells

Species reactivity

General

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

ELISA: 1:2000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000

Target

Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

PubChem: 65396

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Hpu24

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with Hypusine antibody (RGK08101) at 1ug/ml.

    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: K562 cell lysate
    Lane 4: PC-12 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: MCF-7 cell lysate
    Lane 7: 293T cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 17 kDa
    Observed MW: 17 KDa
  • SEC-HPLC
    The purity of this product is >95% as determined by SEC-HPLC.
References

Structural Analysis and Optimization of Context-Independent Anti-Hypusine Antibodies. PMID: 26778617

Characterization of a novel polyclonal anti-hypusine antibody. PMID: 24024105

Synthesis of reagents for the construction of hypusine and deoxyhypusine peptides and their application as peptidic antigens. PMID: 9748364

Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine and tyrosine and O-glycosylated in Trichomonas vaginalis. PMID: 22212642

Polyamines and their derivatives as modulators in growth and differentiation. PMID: 2697982

Differential expression of genes encoding the hypusine-containing translation initiation factor, eIF-5A, in tobacco. PMID: 1542563

Bifunctional activity of deoxyhypusine synthase/hydroxylase from Trichomonas vaginalis. PMID: 26410361

Subcellular localization of the hypusine-containing eukaryotic initiation factor 5A by immunofluorescent staining and green fluorescent protein tagging. PMID: 12210765

Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine in Saccharomyces cerevisiae. PMID: 8325852

Identification of two novel Trichomonas vaginalis eif-5a genes. PMID: 20060503

eIF5A inhibition influences T cell dynamics in the pancreatic microenvironment of the humanized mouse model of Type 1 Diabetes. PMID: 30733517

Eukaryotic initiation factor 5A-1 (eIF5A-1) as a diagnostic marker for aberrant proliferation in intraepithelial neoplasia of the vulva. PMID: 15262146

Higher activity of recombinant bovine deoxyhypusine synthase vs. human deoxyhypusine synthase. PMID: 15039063

cDNA and derived amino acid sequence of the hypusine containing protein from Dictyostelium discoideum. PMID: 2540042

Cloning, expression and functional activity of deoxyhypusine synthase from Plasmodium vivax. PMID: 17042947

Blocking eIF5A modification in cervical cancer cells alters the expression of cancer-related genes and suppresses cell proliferation. PMID: 24220243

Inhibition of multidrug-resistant HIV-1 by interference with cellular S-adenosylmethionine decarboxylase activity. PMID: 16941339

Deoxyhypusine synthase from rat testis: purification and characterization. PMID: 7721768

The N-terminal region of eukaryotic translation initiation factor 5A signals to nuclear localization of the protein. PMID: 17707773

Inhibition of CD83 cell surface expression during dendritic cell maturation by interference with nuclear export of CD83 mRNA. PMID: 10790432

Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1. PMID: 10381392

Mass spectrometrical analysis of bilin-binding protein from the wing of Hebomoia glaucippe (Linnaeus, 1758) (Lepidoptera: Pieridae). PMID: 22740467

Detection of the hypusine-containing protein (HP = eIF-5A) in crude yeast extracts by two-dimensional western blots. PMID: 1281091

Helicobacter pylori in gastric cancer and peptic ulcer disease in a Colombian population. Strain heterogeneity and antibody profiles. PMID: 11683922

A dual-function [Ru(bpy)3]2+ encapsulated metal organic framework for ratiometric Al3+ detection and anticounterfeiting application. PMID: 36866710

Hypusine, a polyamine-derived amino acid critical for eukaryotic translation. PMID: 30257869

Post-translational formation of hypusine in eIF5A: implications in human neurodevelopment. PMID: 34273022

Role of Polyamines and Hypusine in β Cells and Diabetes Pathogenesis. PMID: 35448531

The hypusine-containing translation factor eIF5A. PMID: 25029904

Hypusine: its post-translational formation in eukaryotic initiation factor 5A and its potential role in cellular regulation. PMID: 8347280

Dietary spermidine improves cognitive function. PMID: 33852843

Hypusine: a new target for therapeutic intervention in diabetic inflammation. PMID: 20670594

Is hypusine essential for eukaryotic cell proliferation? PMID: 8108861

Polyamine metabolism is a central determinant of helper T cell lineage fidelity. PMID: 34216540

Functional significance of eIF5A and its hypusine modification in eukaryotes. PMID: 19997760

Targeting the polyamine-hypusine circuit for the prevention and treatment of cancer. PMID: 27357307

Spermidine-induced hypusination preserves mitochondrial and cognitive function during aging. PMID: 34105442

Hypusine is essential for eukaryotic cell proliferation. PMID: 9285094

Hypusine modification of the ribosome-binding protein eIF5A, a target for new anti-inflammatory drugs: understanding the action of the inhibitor GC7 on a murine macrophage cell line. PMID: 23701550

Posttranslational synthesis of hypusine: evolutionary progression and specificity of the hypusine modification. PMID: 17476569

Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. PMID: 31130465

Translation Factor eIF5A, Modification with Hypusine and Role in Regulation of Gene Expression. eIF5A as a Target for Pharmacological Interventions. PMID: 30208826

Deoxyhypusine synthase promotes a pro-inflammatory macrophage phenotype. PMID: 34496231

YAP/TAZ drives cell proliferation and tumour growth via a polyamine-eIF5A hypusination-LSD1 axis. PMID: 35177822

Nutraceutical and Dietary Strategies for Up-Regulating Macroautophagy. PMID: 35216170

Inhibition of hypusine biosynthesis in plasmodium: a possible, new strategy in prevention and therapy of malaria. PMID: 17100634

Polyamines reverse immune senescence via the translational control of autophagy. PMID: 31679458

The post-translational synthesis of a polyamine-derived amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF5A). PMID: 16452303

Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. PMID: 31474573

Hypusine biosynthesis in protein and its biological consequences. PMID: 3151230

Biochemistry and function of hypusine formation on eukaryotic initiation factor 5A. PMID: 9285092

Pharmacological targeting of polyamine and hypusine biosynthesis reduces tumour activity of endometrial cancer. PMID: 35100927

Half Way to Hypusine-Structural Basis for Substrate Recognition by Human Deoxyhypusine Synthase. PMID: 32235505

At the hypusine of the crime. PMID: 22763665

Hypusine biosynthesis in β cells links polyamine metabolism to facultative cellular proliferation to maintain glucose homeostasis. PMID: 31796630

The effect of hypusine modification on the intracellular localization of eIF5A. PMID: 19379712

Arginine metabolism regulates human erythroid differentiation through hypusination of eIF5A. PMID: 36735910

A hypusine-eIF5A-PEAK1 switch regulates the pathogenesis of pancreatic cancer. PMID: 25261239

eIF5A hypusination, boosted by dietary spermidine, protects from premature brain aging and mitochondrial dysfunction. PMID: 33852845

Biosynthesis of hypusine in eIF-4D precursors. PMID: 3151229

Protein-protein-interaction network organization of the hypusine modification system. PMID: 22888148

Biological Relevance and Therapeutic Potential of the Hypusine Modification System. PMID: 26037925

Spermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression. PMID: 36057633

Bi-allelic variants in DOHH, catalyzing the last step of hypusine biosynthesis, are associated with a neurodevelopmental disorder. PMID: 35858628

Blockade of EIF5A hypusination limits colorectal cancer growth by inhibiting MYC elongation. PMID: 33303756

Hypusine-containing protein eIF5A promotes translation elongation. PMID: 19424157

Polyamines. PMID: 6206782

Growth of studies on hypusine in biological systems. PMID: 9285093

Development of orthogonally protected hypusine for solid-phase peptide synthesis. PMID: 25769022

The polyamine-hypusine circuit controls an oncogenic translational program essential for malignant conversion in MYC-driven lymphoma. PMID: 37070973

Hypusine, N6-(4-amino-2-hydroxybutyl)-2,6-diaminohexanoic acid, in tissue proteins of mammals. PMID: 4748368

Deoxyhypusine hydroxylase as a novel pharmacological target for ischemic stroke via inducing a unique post-translational hypusination modification. PMID: 35007708

Deoxyhypusine synthase, an essential enzyme for hypusine biosynthesis, is required for proper exocrine pancreas development. PMID: 33811703

Modulation by hypusination. PMID: 31534233

Completing the hypusine pathway in Plasmodium. PMID: 19740108

The function of the hypusine-containing proteins of yeast and other eukaryotes is well conserved. PMID: 7969034

Hypusine formation in eukaryotic initiation factor 4D is not reversed when rates or specificity of protein synthesis is altered. PMID: 3119589

Identification of the hypusine-containing protein hy+ as translation initiation factor eIF-4D. PMID: 6403941

Specific and sensitive GC-MS analysis of hypusine, Nε-(4-amino-2-hydroxybutyl)lysine, a biomarker of hypusinated eukaryotic initiation factor eIF5A, and its application to the bi-ethnic ASOS study. PMID: 35243537

Hypusine, a new amino acid occurring in bovine brain. Isolation and structural determination. PMID: 4334286

Crystal Structure of Hypusine-Containing Translation Factor eIF5A Bound to a Rotated Eukaryotic Ribosome. PMID: 27196944

The hypusine cascade promotes cancer progression and metastasis through the regulation of RhoA in squamous cell carcinoma. PMID: 27041563

Hypusine is required for a sequence-specific interaction of eukaryotic initiation factor 5A with postsystematic evolution of ligands by exponential enrichment RNA. PMID: 11060315

Spermidine protects against acute kidney injury by modulating macrophage NLRP3 inflammasome activation and mitochondrial respiration in an eIF5A hypusination-related pathway. PMID: 36058905

Isolation and identification of alpha-(gamma-aminobutyryl)-hypusine. PMID: 3950619

Flexible NAD+ Binding in Deoxyhypusine Synthase Reflects the Dynamic Hypusine Modification of Translation Factor IF5A. PMID: 32752130

The mammalian hypusine-containing protein, eukaryotic initiation factor 4D. Structural homology of this protein from several species. PMID: 6423643

Distribution of alpha-(gamma-aminobutyryl)-hypusine. PMID: 3100722

Functions of Polyamines in Mammals. PMID: 27268251

Down-regulation of hypusine biosynthesis in Plasmodium by inhibition of S-adenosyl-methionine-decarboxylase. PMID: 19949824

Hypusine formation in protein by a two-step process in cell lysates. PMID: 3117792

A rapid and robust assay for the determination of the amino acid hypusine as a possible biomarker for a high-throughput screening of antimalarials and for the diagnosis and therapy of different diseases. PMID: 21360085

Synergistic drug combination GC7/DFMO suppresses hypusine/spermidine-dependent eIF5A activation and induces apoptotic cell death in neuroblastoma. PMID: 29295892

Structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A. PMID: 27306458

Polyamines in Eukaryotes, Bacteria, and Archaea. PMID: 27268252

The biosynthesis of protein-bound hypusine (N epsilon -(4-amino-2-hydroxybutyl)lysine). Lysine as the amino acid precursor and the intermediate role of deoxyhypusine (N epsilon -(4-aminobutyl)lysine). PMID: 6806267

Hypusine modification for growth is the major function of spermidine in Saccharomyces cerevisiae polyamine auxotrophs grown in limiting spermidine. PMID: 18451031

Regulation of biosynthesis of hypusine in Chinese hamster ovary cells. Evidence for eIF-4D precursor polypeptides. PMID: 3114263

Recessive Rare Variants in Deoxyhypusine Synthase, an Enzyme Involved in the Synthesis of Hypusine, Are Associated with a Neurodevelopmental Disorder. PMID: 30661771

A rapid and sensitive method for the determination of hypusine in proteins and its distribution and developmental changes. PMID: 6430351

Identification of hypusine, an unusual amino acid, in a protein from human lymphocytes and of spermidine as its biosynthetic precursor. PMID: 6789324

Inactivation of eukaryotic initiation factor 5A (eIF5A) by specific acetylation of its hypusine residue by spermidine/spermine acetyltransferase 1 (SSAT1). PMID: 20942800

Eukaryotic initiation factor 5A hypusine as a negative regulator of adenosine 2B receptor (A2bAR) through interaction with stem loop sequences within the A2bAR 3'-untranslated region. PMID: 36689050

Dramatic attenuation of hypusine formation on eukaryotic initiation factor 5A during senescence of IMR-90 human diploid fibroblasts. PMID: 9066781

Cloning and characterization of hypusine-containing protein eIF5A from the olive flounder Paralichthys olivaceus. PMID: 19344779

Excretion of hypusine by children and by patients with familial hyperlysinemia. PMID: 4753051

Cryptosporidium parvum has an active hypusine biosynthesis pathway. PMID: 24893338

Eukaryotic initiation factor 4D, the hypusine-containing protein, is conserved among eukaryotes. PMID: 3119588

The function of spermine. PMID: 24395705

Modulation of protein synthesis by polyamines. PMID: 25906835

Marked elevation of hypusine formation activity on eukaryotic initiation factor 5A in v-HA-RAS transformed mouse NIH3T3 cells. PMID: 9149130

Changes in eIF-4D hypusine modification or abundance are not correlated with translational repression in HeLa cells. PMID: 3091607

Posttranslational formation of hypusine in a single major protein occurs generally in growing cells and is associated with activation of lymphocyte growth. PMID: 6817926

Tissue transglutaminase expression affects hypusine metabolism in BALB/c 3T3 cells. PMID: 9804167

The archaebacterial hypusine-containing protein. Structural features suggest common ancestry with eukaryotic translation initiation factor 5A. PMID: 1541288

Distribution of hypusine, N 6 -(4-amino-2-hydroxybutyl)-2,6-diaminohexanoic acid, in mammalian organs. PMID: 5141831

Hypusine modification in eukaryotic initiation factor 5A in rodent cells selected for resistance to growth inhibition by ornithine decarboxylase-inhibiting drugs. PMID: 8947467

Development of a Hypusine Reagent for Peptide Synthesis. PMID: 11671715

The polyamine-derived amino acid hypusine: its post-translational formation in eIF-5A and its role in cell proliferation. PMID: 24178473

The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice. PMID: 20501948

Spermidine mediates acetylhypusination of RIPK1 to suppress diabetes onset and progression. [RGK08101]

Novel deoxyhypusine synthase (DHPS) inhibitors target hypusination-induced vasculogenic mimicry (VM) against malignant melanoma. [RGK08101]

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

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Anti-Hypusine antibody (Hpu24) [RGK08101]
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