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Recombinant ASFV Phosphoprotein p30/Pret-105 Protein, N-His (YVV02501)

Applications:ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system:E. coli
Accession:P0C9Z3
Protein length:Met1-Lys186
Overview

Catalog No.

YVV02501

Expression system

E. coli

Species

African swine fever virus (ASFV)

Protein length

Met1-Lys186

Predicted molecular weight

23.48 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

P0C9Z3

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Phosphoprotein p30, p30, Phosphoprotein p32, p32, Pret-105

Data Image
  • SDS-PAGE
    SDS PAGE for recombinant African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV) Phosphoprotein p30/Pret-105 protein
References

ER-directed TREX1 limits cGAS activation at micronuclei. PMID: 33476576

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein. PMID: 33479198

An atlas of substrate specificities for the human serine/threonine kinome. PMID: 36631611

TREX1 as a Novel Immunotherapeutic Target. PMID: 33868310

cGAS phase separation inhibits TREX1-mediated DNA degradation and enhances cytosolic DNA sensing. PMID: 33606975

Cellular energy stress induces AMPK-mediated regulation of YAP and the Hippo pathway. PMID: 25751140

Regenerative Reprogramming of the Intestinal Stem Cell State via Hippo Signaling Suppresses Metastatic Colorectal Cancer. PMID: 32730753

Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription. PMID: 32259481

Structural basis of human TREX1 DNA degradation and autoimmune disease. PMID: 35879334

Disease-Causing Mutations in SF3B1 Alter Splicing by Disrupting Interaction with SUGP1. PMID: 31474574

Phase I First-in-Human Dose Escalation Study of the oral SF3B1 modulator H3B-8800 in myeloid neoplasms. PMID: 34172893

Phosphoprotein-based biomarkers as predictors for cancer therapy. PMID: 32690709

DARPP-32 40 years later. PMID: 33706939

4E-BP1 as an oncotarget. PMID: 26298157

Expression of a recombinant ASFV P30 protein and production of monoclonal antibodies. PMID: 37026062

Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. PMID: 24719451

The extensive and functionally uncharacterized mitochondrial phosphoproteome. PMID: 34144036

Impact of SF3B1 mutation in myelofibrosis. PMID: 35787095

TAZ/YAP fusion proteins: mechanistic insights and therapeutic opportunities. PMID: 36096997

WNKs in Wnt/β-catenin signaling. PMID: 24241206

Functional characterization of co-phosphorylation networks. PMID: 35731218

KIBRA promotes prostate cancer cell proliferation and motility. PMID: 27220053

Recombinant Protein p30 for Serological Diagnosis of African Swine Fever by Immunoblotting Assay. PMID: 27390151

The double life of Irs. PMID: 18590687

Characterization of a novel Hoxa5eGFP mouse line. PMID: 36577717

Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. PMID: 28017544

BRCA1/BARD1-dependent ubiquitination of NF2 regulates Hippo-YAP1 signaling. PMID: 30918126

A colloidal gold test strip assay for the detection of African swine fever virus based on two monoclonal antibodies against P30. PMID: 33495899

The role of vasodilator-stimulated phosphoprotein (VASP) in the control of hepatic gluconeogenic gene expression. PMID: 31017943

Proteomics analysis identifies PEA-15 as an endosomal phosphoprotein that regulates α5β1 integrin endocytosis. PMID: 34615962

Emerging roles of DMP1 in lung cancer. PMID: 18559489

Vasodilator-stimulated phosphoprotein protects against vascular inflammation and insulin resistance. PMID: 25117404

High-Throughput Phosphorylation Screening and Validation through Ti(IV)-Nanopolymer Functionalized Reverse Phase Phosphoprotein Array. PMID: 30103608

SPECC1L regulates palate development downstream of IRF6. PMID: 31943082

Fascin1 empowers YAP mechanotransduction and promotes cholangiocarcinoma development. PMID: 34155338

Oxidative tissue: perilipin 5 links storage with the furnace. PMID: 21632259

CAS proteins in normal and pathological cell growth control. PMID: 19937461

Role of adipose specific lipid droplet proteins in maintaining whole body energy homeostasis. PMID: 23688782

A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. PMID: 23233447

Preclinical and clinical studies of the NEDD9 scaffold protein in cancer and other diseases. PMID: 25967390

Genomic Integrity Safeguards Self-Renewal in Embryonic Stem Cells. PMID: 31390555

TREX1 - Apex predator of cytosolic DNA metabolism. PMID: 32615442

Perilipins: lipid droplet coat proteins adapted for tissue-specific energy storage and utilization, and lipid cytoprotection. PMID: 24036367

Embryonal Fyn-associated substrate (EFS) and CASS4: The lesser-known CAS protein family members. PMID: 26119091

A new central scaffold for metastasis: parsing HEF1/Cas-L/NEDD9. PMID: 17908996

cAMP-regulated phosphoproteins DARPP-32, ARPP16/19, and RCS modulate striatal signal transduction through protein kinases and phosphatases. PMID: 33706938

SMIM1 is a type II transmembrane phosphoprotein and displays the Vel blood group antigen at its carboxyl-terminus. PMID: 26452714

Characterization of p30, a highly antigenic membrane and secreted protein of African swine fever virus. PMID: 1604821

Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide. PMID: 34715492

African swine fever virus protein p30 interaction with heterogeneous nuclear ribonucleoprotein K (hnRNP-K) during infection. PMID: 18775702

The Connector Domain of Vesicular Stomatitis Virus Large Protein Interacts with the Viral Phosphoprotein. PMID: 31896592

Backbone and side-chain chemical shift assignments of full-length, apo, human Pin1, a phosphoprotein regulator with interdomain allostery. PMID: 30353504

Efficient inhibition of African swine fever virus replication by CRISPR/Cas9 targeting of the viral p30 gene (CP204L). PMID: 29362418

Ultrastructural organization of dentin in mice lacking dentin sialo-phosphoprotein. PMID: 25158189

Subcellular Localization of Histidine Phosphorylated Proteins Through Indirect Immunofluorescence. PMID: 31707661

Identification of Microprotein-Protein Interactions via APEX Tagging. PMID: 28589727

Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins. PMID: 19638644

IQGAP1 and RNA Splicing in the Context of Head and Neck via Phosphoproteomics. PMID: 35980772

Methods for investigation of targeted kinase inhibitor therapy using chemical proteomics and phosphorylation profiling. PMID: 20361944

Structures of the FXYD regulatory proteins in lipid micelles and membranes. PMID: 18000745

The in vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis. PMID: 7951750

HEF1-aurora A interactions: points of dialog between the cell cycle and cell attachment signaling networks. PMID: 16479169

Use of stable isotope labeling by amino acids in cell culture (SILAC) for phosphotyrosine protein identification and quantitation. PMID: 19241007

Analysis of the Candida albicans Phosphoproteome. PMID: 25750214

Hypophosphatemic osteosclerosis, hyperostosis, and enthesopathy associated with novel homozygous mutations of DMP1 encoding dentin matrix protein 1 and SPP1 encoding osteopontin: The first digenic SIBLING protein osteopathy? PMID: 31843680

Application of a monoclonal antibody recognizing protein p30 to detect African swine fever virus-infected cells in peripheral blood. PMID: 8609199

Molecular basis for HEF1/NEDD9/Cas-L action as a multifunctional co-ordinator of invasion, apoptosis and cell cycle. PMID: 17703068

Vasodilator-stimulated phosphoprotein promotes activation of hepatic stellate cells by regulating Rab11-dependent plasma membrane targeting of transforming growth factor beta receptors. PMID: 24917558

Retinophilin is a light-regulated phosphoprotein required to suppress photoreceptor dark noise in Drosophila. PMID: 20107052

Phosphoproteomic analysis: an emerging role in deciphering cellular signaling in human embryonic stem cells and their differentiated derivatives. PMID: 22009073

The African swine fever virus proteins p54 and p30 are involved in two distinct steps of virus attachment and both contribute to the antibody-mediated protective immune response. PMID: 9568043

Growth-dependent podocyte failure causes glomerulosclerosis. PMID: 22773827

Nopp140-mediated concentration of telomerase in Cajal bodies regulates telomere length. PMID: 31664887

The family of retinoblastoma proteins. PMID: 11693966

Selectivity in subunit composition of Ena/VASP tetramers. PMID: 26221026

SPLUNC1 Loses Its Antimicrobial Activity in Acidic Cystic Fibrosis Airway Secretions. PMID: 31013116

MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells. PMID: 34031396

Oncogenic KRAS Regulates Tumor Cell Signaling via Stromal Reciprocation. PMID: 27087446

Palmitoylation stabilizes unliganded rod opsin. PMID: 20404157

α-Catenin-dependent cytoskeletal tension controls Yap activity in the heart. PMID: 29467248

YAP-mediated induction of monoacylglycerol lipase restrains oncogenic transformation. PMID: 25636199

Pathologic Spectrum and Molecular Landscape of Myeloid Disorders Harboring SF3B1 Mutations. PMID: 33978189

Modeling SF3B1 Mutations in Cancer: Advances, Challenges, and Opportunities. PMID: 27622329

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

Contact Information

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For research use only. Not for human or drug use.

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Recombinant ASFV Phosphoprotein p30/Pret-105 Protein, N-His [YVV02501]
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