Catalog No.
YVV01801
Expression system
E. coli
Species
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Protein length
Ala5325-Gln5925
Predicted molecular weight
69.15 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P0DTD1
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in 50mM Tris-HCl, 0.02% NLS, pH 7.5, 5µM ZnCl2, 2mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Helicase, Hel, Non-structural protein 13, nsp13, Replicase polyprotein 1ab, pp1ab, ORF1ab polyprotein
SARS-CoV-2 point mutations are over-represented in terminal loops of RNA stem-loop structures that can be resolved by Nsp13 helicase in a unique manner with respect to nucleotide dependence., PMID:40421800
Myricetin-bound crystal structure of the SARS-CoV-2 helicase NSP13 facilitates the discovery of novel natural inhibitors., PMID:40421686
Analysis of the SARS-CoV-2 inactivation mechanism using violet-blue light (405 nm)., PMID:40366184
The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14., PMID:40358207
Structural Basis of SARS-CoV-2 Nsp13-Derived Peptide-Mediated NK Cell Activation., PMID:40331402
Structure-Activity Relationships of New 1-Aryl-1H-Indole Derivatives as SARS-CoV-2 Nsp13 Inhibitors., PMID:40322961
Chemical arsenal for helicase Hunters: Striking the toughest targets in antiviral research., PMID:40316178
Acute COVID-19 and LongCOVID syndrome - molecular implications for therapeutic strategies - review., PMID:40313948
Monitoring SARS-CoV-2 Nsp13 helicase binding activity using expanded genetic code techniques., PMID:40309067
Discovery of 4-((quinolin-8-ylthio)methyl)benzamide derivatives as a new class of SARS-CoV-2 nsp13 inhibitors., PMID:40147803
SARS-CoV-2 Nsp13 helicase modulates miR-146a-mediated signaling pathways., PMID:40073498
Duplex Unwinding Mechanism of Coronavirus MERS-CoV nsp13 Helicase., PMID:40018651
Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures., PMID:39936915
Analogs of NIH Molecular Probe ML283 Are Potent SARS-CoV-2 Helicase Inhibitors., PMID:39910979
A novel ADP-directed chaperone function facilitates the ATP-driven motor activity of SARS-CoV helicase., PMID:39878212
SARS-CoV-2 resistance analyses from the Phase 3 PINETREE study of remdesivir treatment in nonhospitalized participants., PMID:39699245
Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling., PMID:39697597
Mutational and evolutionary dynamics of non-structural and spike proteins from variants of concern (VOC) of SARS-CoV-2 in India., PMID:39488303
SARS-CoV-2 nsp13 suppresses hepatitis B virus replication by targeting cccDNA transcription., PMID:39373477
High throughput screening for SARS-CoV-2 helicase inhibitors., PMID:39173831
Variable Inhibition of DNA Unwinding Rates Catalyzed by the SARS-CoV-2 Helicase Nsp13 by Structurally Distinct Single DNA Lesions., PMID:39063172
A Repurposed Drug Interferes with Nucleic Acid to Inhibit the Dual Activities of Coronavirus Nsp13., PMID:38980755
Mechanistic insights into bismuth(iii) inhibition of SARS-CoV-2 helicase., PMID:38966375
Progressive Evolutionary Dynamics of Gene-Specific ω Led to the Emergence of Novel SARS-CoV-2 Strains Having Super-Infectivity and Virulence with Vaccine Neutralization., PMID:38928018
Mutation prediction in the SARS-CoV-2 genome using attention-based neural machine translation., PMID:38872567
Generalized open-source workflows for atomistic molecular dynamics simulations of viral helicases., PMID:38869150
Heterotypic immunity from prior SARS-CoV-2 infection but not COVID-19 vaccination associates with lower endemic coronavirus incidence., PMID:38865483
The Coronavirus helicase in replication., PMID:38796132
Development of a Fluorescent Assay and Imidazole-Containing Inhibitors by Targeting SARS-CoV-2 Nsp13 Helicase., PMID:38792162
Genomic Context of SARS-CoV-2 Outbreaks in Farmed Mink in Spain during Pandemic: Unveiling Host Adaptation Mechanisms., PMID:38791536
The Dual-Targeted Fusion Inhibitor Clofazimine Binds to the S2 Segment of the SARS-CoV-2 Spike Protein., PMID:38675980
Drug repurposing screen to identify inhibitors of the RNA polymerase (nsp12) and helicase (nsp13) from SARS-CoV-2 replication and transcription complex., PMID:38490582
Identification of Potential Inhibitors of the SARS-CoV-2 NSP13 Helicase via Structure-Based Ligand Design, Molecular Docking and Nonequilibrium Alchemical Simulations., PMID:38456332
The PKA-CREB1 axis regulates coronavirus proliferation by viral helicase nsp13 association., PMID:38445884
Activity and inhibition of the SARS-CoV-2 Omicron nsp13 R392C variant using RNA duplex unwinding assays., PMID:38301954
Analysis of SARS-CoV-2 genome evolutionary patterns., PMID:38197644
Role of ATP Hydrolysis and Product Release in the Translocation Mechanism of SARS-CoV-2 NSP13., PMID:38175211
Heterotypic responses against nsp12/nsp13 from prior SARS-CoV-2 infection associates with lower subsequent endemic coronavirus incidence., PMID:37961343
The Role of ATP Hydrolysis and Product Release in the Translocation Mechanism of SARS-CoV-2 NSP13., PMID:37808802
SARS-CoV-2 Nsp8 N-terminal domain folds autonomously and binds dsRNA., PMID:37665006
Diketo acid inhibitors of nsp13 of SARS-CoV-2 block viral replication., PMID:37562607
Observing inhibition of the SARS-CoV-2 helicase at single-nucleotide resolution., PMID:37560916
An iron-sulfur cluster in the zinc-binding domain of the SARS-CoV-2 helicase modulates its RNA-binding and -unwinding activities., PMID:37552760
An overview of anti-SARS-CoV-2 and anti-inflammatory potential of baicalein and its metabolite baicalin: Insights into molecular mechanisms., PMID:37437351
Identification of a 1.4-kb-Long Sequence Located in the nsp12 and nsp13 Coding Regions of SARS-CoV-2 Genomic RNA That Mediates Efficient Viral RNA Packaging., PMID:37367225
An immunoinformatics approach to study the epitopes of SARS-CoV-2 helicase, Nsp13., PMID:37362836
SARS-CoV-2 nsp13 Restricts Episomal DNA Transcription without Affecting Chromosomal DNA., PMID:37347173
SARS-CoV-2 intrahost evolution in immunocompromised patients in comparison with immunocompetent populations after treatment., PMID:37341553
A mutation in the coronavirus nsp13-helicase impairs enzymatic activity and confers partial remdesivir resistance., PMID:37338298
SARS-CoV-2 NSP13 interacts with host IRF3, blocking antiviral immune responses., PMID:37314155
For research use only. Not for human or drug use.
