Catalog No.
YVV01601
Expression system
E. coli
Species
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Protein length
Ala4254-Ser4393
Predicted molecular weight
17.99 kDa
Nature
Recombinant
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level
Please contact with the lab for this information.
Purity
>80% as determined by SDS-PAGE.
Accession
P0DTD1
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Non-structural protein 10, nsp10, Growth factor-like peptide, GFL, Replicase polyprotein 1ab, pp1ab, ORF1ab polyprotein
Note
For research use only.
Crystal structure of SARS-CoV-2 nsp10-nsp16 in complex with small molecule inhibitors, SS148 and WZ16., PMID:36040262
SIRT5 is a proviral factor that interacts with SARS-CoV-2 Nsp14 protein., PMID:36095012
Expediting the drug discovery for ideal leads against SARS-CoV-2 via molecular docking of repurposed drugs., PMID:36165445
Persistent Nonviral Plasmid Vector in Nasal Tissues Causes False-Positive SARS-CoV-2 Diagnostic Nucleic Acid Tests., PMID:36226962
Identification and characterization of alternative sites and molecular probes for SARS-CoV-2 target proteins., PMID:36385993
Computational Analysis of SAM Analogs as Methyltransferase Inhibitors of nsp16/nsp10 Complex from SARS-CoV-2., PMID:36430451
Interfering with nucleotide excision by the coronavirus 3'-to-5' exoribonuclease., PMID:36546762
Crystal structures and fragment screening of SARS-CoV-2 NSP14 reveal details of exoribonuclease activation and mRNA capping and provide starting points for antiviral drug development., PMID:36546776
Analysis of critical protein-protein interactions of SARS-CoV-2 capping and proofreading molecular machineries towards designing dual target inhibitory peptides., PMID:36611052
Targeting viral proteins for restraining SARS-CoV-2: focusing lens on viral proteins beyond spike for discovering new drug targets., PMID:36723288
SS148 and WZ16 inhibit the activities of nsp10-nsp16 complexes from all seven human pathogenic coronaviruses., PMID:36764586
Global landscape of SARS-CoV-2 mutations and conserved regions., PMID:36841805
In vitro and in silico evaluation of antiretrovirals against SARS-CoV-2: A drug repurposing approach., PMID:36891537
Design and synthesis of naturally-inspired SARS-CoV-2 inhibitors., PMID:36970153
SARS-CoV-2 polyprotein substrate regulates the stepwise Mpro cleavage reaction., PMID:37044215
A universal fluorescence polarization high throughput screening assay to target the SAM-binding sites of SARS-CoV-2 and other viral methyltransferases., PMID:37060263
Integrated molecular and quantum mechanical approach to identify novel potent natural bioactive compound against 2'-O-methyltransferase (nsp16) of SARS-CoV-2., PMID:37129206
A Non-Structural Protein of Severe Acute Respiratory Syndrome Coronavirus 2 with a Potential Ability to Reduce Blood Glucose for Use in Controlling Type 2 Diabetes Mellitus., PMID:37274904
Activity of nsp14 Exonuclease from SARS-CoV-2 towards RNAs with Modified 3'-Termini., PMID:37340295
AI-guided pipeline for protein-protein interaction drug discovery identifies a SARS-CoV-2 inhibitor., PMID:37398436
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering., PMID:37686452
Resolving a guanine-quadruplex structure in the SARS-CoV-2 genome through circular dichroism and multiscale molecular modeling., PMID:37886086
Development of Pan-Anti-SARS-CoV-2 Agents through Allosteric Inhibition of nsp14/nsp10 Complex., PMID:37897418
A repository of COVID-19 related molecular dynamics simulations and utilisation in the context of nsp10-nsp16 antivirals., PMID:37976980
An exonuclease-resistant chain-terminating nucleotide analogue targeting the SARS-CoV-2 replicase complex., PMID:38096103
Emerging variants of SARS-CoV-2 NSP10 highlight strong functional conservation of its binding to two non-structural proteins, NSP14 and NSP16., PMID:38127066
SARS-CoV-2 NSP14 MTase activity is critical for inducing canonical NF-κB activation., PMID:38131452
5'-cap RNA/SAM mimetic conjugates as bisubstrate inhibitors of viral RNA cap 2'-O-methyltransferases., PMID:38199140
Reconstitution of RNA cap methylation reveals different features of SARS-CoV-2 and SARS-CoV methyltransferases., PMID:38285434
New insights into complex formation by SARS-CoV-2 nsp10 and nsp14., PMID:38422227
AI-guided pipeline for protein-protein interaction drug discovery identifies a SARS-CoV-2 inhibitor., PMID:38467836
Despite the odds: formation of the SARS-CoV-2 methylation complex., PMID:38499483
Native Mass Spectrometry Dissects the Structural Dynamics of an Allosteric Heterodimer of SARS-CoV-2 Nonstructural Proteins., PMID:38535992
An entropy-based study on the mutational landscape of SARS-CoV-2 in USA: Comparing different variants and revealing co-mutational behavior of proteins., PMID:38754568
Structure-Based Virtual Screening for Methyltransferase Inhibitors of SARS-CoV-2 nsp14 and nsp16., PMID:38792173
Progressive Evolutionary Dynamics of Gene-Specific ω Led to the Emergence of Novel SARS-CoV-2 Strains Having Super-Infectivity and Virulence with Vaccine Neutralization., PMID:38928018
Structural and functional insights into the 2'-O-methyltransferase of SARS-CoV-2., PMID:38969340
Comparative Atlas of SARS-CoV-2 Substitution Mutations: A Focus on Iranian Strains Amidst Global Trends., PMID:39205305
Structural Basis for Inhibition of the SARS-CoV-2 nsp16 by Substrate-Based Dual Site Inhibitors., PMID:39258386
Biochemical simulation of mutation synthesis and repair during SARS-CoV-2 RNA polymerization., PMID:39366027
Identification of adenosine analogues as nsp14 N7‑methyltransferase inhibitors for treating coronaviruses infection., PMID:39490138
Cannabinoid-Inspired Inhibitors of the SARS-CoV-2 Coronavirus 2'-O-Methyltransferase (2'-O-MTase) Non-Structural Protein (Nsp10-16)., PMID:39519722
Dynamic Combinatorial Chemistry Unveils Nsp10 Inhibitors with Antiviral Potential Against SARS-CoV-2., PMID:39676060
SARS-CoV-2 resistance analyses from the Phase 3 PINETREE study of remdesivir treatment in nonhospitalized participants., PMID:39699245
Screening of RNA methyltransferase NSP16 inhibitors against SARS-CoV-2 coronavirus and study of related mechanisms., PMID:40092096
Mutagenesis-Guided Target Identification Reveals the Protein-Binding Domain of Nsp14 in Coronaviruses as the Target of a Labdane-Oxindole Compound., PMID:40207883
Structural insights into the assembly and regulation of 2'-O RNA methylation by SARS-CoV-2 nsp16/nsp10., PMID:40220753
Transfer learning towards predicting viral missense mutations: A case study on SARS-CoV-2., PMID:40352476
SARS-CoV-2 nsp16 is regulated by host E3 ubiquitin ligases, UBR5 and MARCHF7., PMID:40358464
Structural Basis and Rational Design of Nucleotide Analogue Inhibitor Evading the SARS-CoV-2 Proofreading Enzyme., PMID:40509573
For research use only. Not for human or drug use.
