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Recombinant SARS-CoV-2 NSP8 Protein, N-His

Catalog #:   YVV01401 Specific References (50) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: P0DTD1
Protein length: Ala3943-Gln4140
Overview

Catalog No.

YVV01401

Expression system

E. coli

Species

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Protein length

Ala3943-Gln4140

Predicted molecular weight

24.18 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

P0DTD1

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Non-structural protein 8, nsp8, Replicase polyprotein 1ab, pp1ab, ORF1ab polyprotein

Data Image
  • SDS-PAGE
    SDS PAGE for recombinant SARS-CoV-2 NSP8
References

Genome-Wide Interrogation of SARS-CoV-2 RNA-Protein Interactions Uncovers Hidden Regulatory Sites., PMID:40501555

Unveiling SARS-CoV-2's heart: role, structure and inhibition of SARS-CoV-2 RNA-dependent RNA polymerase., PMID:40473220

Single-Molecule Assay Reveals Binding Dynamics of SARS-CoV-2 Polymerase Components and Provides a New Tool to Distinguish Polymerase Inhibitors., PMID:40465830

A post-assembly conformational change makes the SARS-CoV-2 polymerase elongation-competent., PMID:40464687

HERC5-mediated ISGylation of SARS-CoV-2 nsp8 facilitates its degradation and inhibits viral replication., PMID:40409630

Targeting CTP synthetase 1 to restore interferon induction and impede nucleotide synthesis in SARS-CoV-2 infection., PMID:40298378

Mechanistic insights into the activity of SARS-CoV-2 RNA polymerase inhibitors using single-molecule FRET., PMID:40298114

In Silico and In Vitro development of novel small interfering RNAs (siRNAs) to inhibit SARS-CoV-2., PMID:40256168

A novel replicase-mediated self-amplifying RNA amplification mechanism of the SARS-CoV-2 replication-transcription system., PMID:40117978

A post-assembly conformational change makes the SARS-CoV-2 polymerase elongation-competent., PMID:39829827

Single-molecule assay reveals the impact of composition, RNA duplex, and inhibitors on the binding dynamics of SARS-CoV-2 polymerase complex., PMID:39829757

Discovery of a 2'-α-Fluoro-2'-β-C-(fluoromethyl) Purine Nucleotide Prodrug as a Potential Oral Anti-SARS-CoV-2 Agent., PMID:39804580

SARS-CoV-2 resistance analyses from the Phase 3 PINETREE study of remdesivir treatment in nonhospitalized participants., PMID:39699245

Biochemical simulation of mutation synthesis and repair during SARS-CoV-2 RNA polymerization., PMID:39366027

Characterization of the Conformational Hotspots of the RNA-Dependent RNA Polymerase Complex Identifies a Unique Structural Malleability of nsp8., PMID:39356135

Aggravating mechanisms from COVID-19., PMID:39334442

Assessing the inhibitory effects of some secondary amines, thioureas and 1,3-dimethyluracil conjugates of (-)-cytisine and thermopsine on the RNA-dependent RNA polymerase of SARS-CoV-1 and SARS-CoV-2., PMID:39251111

Mapping the immunopeptidome of seven SARS-CoV-2 antigens across common HLA haplotypes., PMID:39214998

Comparative Atlas of SARS-CoV-2 Substitution Mutations: A Focus on Iranian Strains Amidst Global Trends., PMID:39205305

Biochemical characterization of naturally occurring mutations in SARS-CoV-2 RNA-dependent RNA polymerase., PMID:39145418

Point mutations at specific sites of the nsp12-nsp8 interface dramatically affect the RNA polymerization activity of SARS-CoV-2., PMID:38990941

Progressive Evolutionary Dynamics of Gene-Specific ω Led to the Emergence of Novel SARS-CoV-2 Strains Having Super-Infectivity and Virulence with Vaccine Neutralization., PMID:38928018

Mutations in nonstructural proteins essential for pathogenicity in SARS-CoV-2-infected mice., PMID:38888344

Mutation prediction in the SARS-CoV-2 genome using attention-based neural machine translation., PMID:38872567

Immune responses during COVID-19 breakthrough cases in vaccinated children and adolescents., PMID:38812507

NMR Studies of Genomic RNA in 3' Untranslated Region Unveil Pseudoknot Structure that Initiates Viral RNA Replication in SARS-CoV-2., PMID:38665648

Bio-Chemoinformatics-Driven Analysis of nsp7 and nsp8 Mutations and Their Effects on Viral Replication Protein Complex Stability., PMID:38534781

Optimized Recombinant Expression and Purification of the SARS-CoV-2 Polymerase Complex., PMID:38511495

Drug repurposing screen to identify inhibitors of the RNA polymerase (nsp12) and helicase (nsp13) from SARS-CoV-2 replication and transcription complex., PMID:38490582

An alphacoronavirus polymerase structure reveals conserved replication factor functions., PMID:38442273

Incipient functional SARS-CoV-2 diversification identified through neural network haplotype maps., PMID:38416684

Investigation of protein-protein interactions and hotspot region on the NSP7-NSP8 binding site in NSP12 of SARS-CoV-2., PMID:38304231

Assessing Genomic Mutations in SARS-CoV-2: Potential Resistance to Antiviral Drugs in Viral Populations from Untreated COVID-19 Patients., PMID:38276171

The E3 ligase TRIM22 restricts SARS-CoV-2 replication by promoting proteasomal degradation of NSP8., PMID:38275298

SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitination., PMID:37956198

SARS-CoV-2 viral genes Nsp6, Nsp8, and M compromise cellular ATP levels to impair survival and function of human pluripotent stem cell-derived cardiomyocytes., PMID:37705046

SARS-CoV-2 variants with NSP12 P323L/G671S mutations display enhanced virus replication in ferret upper airways and higher transmissibility., PMID:37676771

RNA-dependent RNA polymerase of SARS-CoV-2 regulate host mRNA translation efficiency by hijacking eEF1A factors., PMID:37673357

SARS-CoV-2 Nsp8 N-terminal domain folds autonomously and binds dsRNA., PMID:37665006

A comprehensive Drosophila resource to identify key functional interactions between SARS-CoV-2 factors and host proteins., PMID:37480566

Peptide inhibitors derived from the nsp7 and nsp8 cofactors of nsp12 targeting different substrate binding sites of nsp12 of the SARS-CoV-2., PMID:37434315

Targeting the vital non-structural proteins (NSP12, NSP7, NSP8 and NSP3) from SARS-CoV-2 and inhibition of RNA polymerase by natural bioactive compound naringenin as a promising drug candidate against COVID-19., PMID:37131962

Drug-Repurposing Screening Identifies a Gallic Acid Binding Site on SARS-CoV-2 Non-structural Protein 7., PMID:37082753

An alphacoronavirus polymerase structure reveals conserved co-factor functions., PMID:36993498

Porcine Epidemic Diarrhea Virus nsp7 Inhibits MDA5 Dephosphorylation to Antagonize Type I Interferon Production., PMID:36975829

SARS-CoV-2 NSP8 suppresses type I and III IFN responses by modulating the RIG-I/MDA5, TRIF, and STING signaling pathways., PMID:36929724

Biophysical Interpretation of Evolutionary Consequences on the SARS-CoV2 Main Protease through Molecular Dynamics Simulations and Network Topology Analysis., PMID:36913683

Recognition and Cleavage of Human tRNA Methyltransferase TRMT1 by the SARS-CoV-2 Main Protease., PMID:36865253

Global landscape of SARS-CoV-2 mutations and conserved regions., PMID:36841805

Phase Separation: The Robust Modulator of Innate Antiviral Signaling and SARS-CoV-2 Infection., PMID:36839515

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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For research use only. Not for human or drug use.

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Recombinant SARS-CoV-2 NSP8 Protein, N-His [YVV01401]
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