Catalog No.
YVV01202
Expression system
E. coli
Species
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Protein length
Glu1564-Ile1877
Predicted molecular weight
38.59 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>85% as determined by SDS-PAGE.
Accession
P0DTD1
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Non-structural protein 3, nsp3, PL2-PRO, Papain-like protease, Papain-like proteinase, PL-PRO, Replicase polyprotein 1ab, pp1ab, ORF1ab polyprotein
Genome-Wide Interrogation of SARS-CoV-2 RNA-Protein Interactions Uncovers Hidden Regulatory Sites., PMID:40501555
Defining short linear motif binding determinants by phage display-based deep mutational scanning., PMID:40411416
Identification of a series of pyrrolo-pyrimidine-based SARS-CoV-2 Mac1 inhibitors that repress coronavirus replication., PMID:40407321
Structural and functional insights into the SARS-CoV-2 SUD domain and its interaction with RNA G-Quadruplexes., PMID:40252399
The SARS-CoV-2 NSP4 T492I mutation promotes double-membrane vesicle formation to facilitate transmission., PMID:40157604
Comparative Interactome Profiling of Nonstructural Protein 3 Across SARS-CoV-2 Variants Emerged During the COVID-19 Pandemic., PMID:40143373
Derivatives of MOPS: promising scaffolds for SARS coronaviruses Macro domain-targeted inhibition., PMID:40070175
Evaluation of Genomic Surveillance of SARS-CoV-2 Virus Isolates and Comparison of Mutational Spectrum of Variants in Bangladesh., PMID:40006937
Exploring natural compounds and synthetic derivatives as potential inhibitors of SARS-CoV-2 PLpro: a computational approach with enzyme inhibition and cytotoxicity assessment., PMID:39972932
Torsional twist of the SARS-CoV and SARS-CoV-2 SUD-N and SUD-M domains., PMID:39969084
Orchestration of SARS-CoV-2 Nsp4 and host cell ESCRT proteins induces morphological changes of the endoplasmic reticulum., PMID:39937675
A comprehensive review of current insights into the virulence factors of SARS-CoV-2., PMID:39878471
Investigation of Immunoreactivity Profiles and Epitope Landscape in Divergent COVID-19 Trajectories and SARS-CoV-2 Variants., PMID:39873496
Exploring pyrazolines as potential inhibitors of NSP3-macrodomain of SARS-CoV-2: synthesis and in silico analysis., PMID:39755743
SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12., PMID:39737877
Regulation of N-degron recognin-mediated autophagy by the SARS-CoV-2 PLpro ubiquitin deconjugase., PMID:39723606
Proteome-Wide Analysis of Antibody Responses in Asymptomatic Omicron BA.2-Infected Individuals at the Amino Acid Resolution., PMID:39661118
SARS-CoV-2 N protein recruits G3BP to double membrane vesicles to promote translation of viral mRNAs., PMID:39638802
Computational Evidence for Bisartan Arginine Blockers as Next-Generation Pan-Antiviral Therapeutics Targeting SARS-CoV-2, Influenza, and Respiratory Syncytial Viruses., PMID:39599890
Covalent DNA-Encoded Library Workflow Drives Discovery of SARS-CoV-2 Nonstructural Protein Inhibitors., PMID:39574309
Phylogeogenomic analysis of the earliest reported sequences of SARS-CoV-2 from 161 countries., PMID:39563179
Identification of a series of pyrrolo-pyrimidine based SARS-CoV-2 Mac1 inhibitors that repress coronavirus replication., PMID:39554145
Mutational and evolutionary dynamics of non-structural and spike proteins from variants of concern (VOC) of SARS-CoV-2 in India., PMID:39488303
MDA5 Is a Major Determinant of Developing Symptoms in Critically Ill COVID-19 Patients., PMID:39460899
Investigating TCR-pMHC interactions for TCRs without identified epitopes by constructing a computational pipeline., PMID:39423970
The SARS-unique domain (SUD) of SARS-CoV-2 nsp3 protein inhibits the antiviral immune responses through the NF-κB pathway., PMID:39400381
The CoV-Y domain of SARS-CoV-2 Nsp3 interacts with BRAP to stimulate NF-κB signaling and induce host inflammatory responses., PMID:39343285
N terminus of SARS-CoV-2 nonstructural protein 3 interrupts RNA-driven phase separation of N protein by displacing RNA., PMID:39341499
SARS-CoV-2 Genotyping Highlights the Challenges in Spike Protein Drift Independent of Other Essential Proteins., PMID:39338537
The impact of host microRNAs on the development of conserved mutations of SARS-CoV-2., PMID:39333651
A Novel Self-Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations., PMID:39313862
Prediction of the effects of the top 10 synonymous mutations from 26645 SARS-CoV-2 genomes of early pandemic phase., PMID:39268187
Extensive exploration of structure activity relationships for the SARS-CoV-2 macrodomain from shape-based fragment merging and active learning., PMID:39253507
Identifying novel chemical matter against the Chikungunya virus nsP3 macrodomain through crystallographic fragment screening., PMID:39229067
Emergence of crucial evidence catalyzing the origin tracing of SARS-CoV-2., PMID:39213297
Comparative Atlas of SARS-CoV-2 Substitution Mutations: A Focus on Iranian Strains Amidst Global Trends., PMID:39205305
ISGylation of the SARS-CoV-2 N protein by HERC5 impedes N oligomerization and thereby viral RNA synthesis., PMID:39194248
Torsional Twist of the SARS-CoV and SARS-CoV-2 SUD-N and SUD-M domains., PMID:39185168
ISGylation of the SARS-CoV-2 N protein by HERC5 impedes N oligomerization and thereby viral RNA synthesis., PMID:39149229
Molecular architecture of coronavirus double-membrane vesicle pore complex., PMID:39143215
Oxysterole-binding protein targeted by SARS-CoV-2 viral proteins regulates coronavirus replication., PMID:39119292
[Self-assembly of the C-terminal and transmembrane region of the SARS-CoV-2 protein nsp3]., PMID:38986091
Synthesis of Structural ADP-Ribose Analogues as Inhibitors for SARS-CoV-2 Macrodomain 1., PMID:38935522
Natural-Target-Mimicking Translocation-Based Fluorescent Sensor for Detection of SARS-CoV-2 PLpro Protease Activity and Virus Infection in Living Cells., PMID:38928340
Progressive Evolutionary Dynamics of Gene-Specific ω Led to the Emergence of Novel SARS-CoV-2 Strains Having Super-Infectivity and Virulence with Vaccine Neutralization., PMID:38928018
Investigating SARS-CoV-2 virus-host interactions and mRNA expression: Insights using three models of D. melanogaster., PMID:38925484
Immunoinformatic of novel self-amplifying mRNA vaccine lipid nanoparticle against SARS-CoV-2., PMID:38903554
Mutation prediction in the SARS-CoV-2 genome using attention-based neural machine translation., PMID:38872567
PARP14 is regulated by the PARP9/DTX3L complex and promotes interferon γ-induced ADP-ribosylation., PMID:38834852
Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organelles., PMID:38821943
For research use only. Not for human or drug use.
