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Anti-ENAH Antibody (R4C59)

Catalog #:   RHP03401 Specific References (24) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IHC, IP, WB
Accession: Q8N8S7
Overview

Catalog No.

RHP03401

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:100-1:200, IP: 1:100-1:200, WB: 1:500-1:1000

Target

ENAH, MENA, Protein enabled homolog

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q8N8S7

Applications

IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R4C59

Data Image
  • Immunohistochemical
    IHC-P analysis of endometrium tissue by ENAH antibody (RHP03401). IHC-P was performed using sections of the formalin-fixed paraffin-embedded endometrium tissue;Result: Glandular cells, vascular endothelial cell and smooth muscle cell are positively stained at the cytoplasm and cell membrane (Only positively stained glandular cells and vascular endothelial cell are shown here).
  • Immunoprecipitation
    Lane 1: U-2 OS lysate ; Lane 2: ENAH immunoprecipitated from U-2 OS lysate by RHP03401;Lane 3: The same as Lane 2 but use IgG isotype control antibody ;Result: RHP03401 can immunoprecipitate ENAH;
  • Western blot
    Western blot analysis using ENAH mouse mAb against U-2 OS lysate
References

Alternative polyadenylation mechanism links secreted phosphoprotein 1 gene to glioblastoma., PMID:35275520

Quantitative serum HBV markers in predicting phases of natural history of chronic HBV infection., PMID:34217779

Clinical Characteristics of Patients with Different N-Terminal Probrain Natriuretic Peptide Levels after Hematopoietic Stem Cell Transplantation., PMID:33133305

The alternatively-included 11a sequence modifies the effects of Mena on actin cytoskeletal organization and cell behavior., PMID:27748415

Hepatitis B, HIV, and Syphilis Seroprevalence in Pregnant Women and Blood Donors in Cameroon., PMID:27578957

Characterization of the expression of the pro-metastatic Mena(INV) isoform during breast tumor progression., PMID:26680363

Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer's Disease., PMID:26417591

hMENA(11a) contributes to HER3-mediated resistance to PI3K inhibitors in HER2-overexpressing breast cancer cells., PMID:25961924

Gastric cancer in young vs old Romanian patients: immunoprofile with emphasis on maspin and mena protein reactivity., PMID:25556597

A pathway-based approach for identifying biomarkers of tumor progression to trastuzumab-resistant breast cancer., PMID:25449779

Invasive breast carcinoma cells from patients exhibit MenaINV- and macrophage-dependent transendothelial migration., PMID:25429076

Quantitative assessment of invasive mena isoforms (Menacalc) as an independent prognostic marker in breast cancer., PMID:22971274

Mena, a new available marker in tumors of salivary glands?, PMID:22472896

The immunohistochemical aspects of protein Mena in cervical lesions., PMID:19434313

The expression of cytoskeleton regulatory protein Mena in colorectal lesions., PMID:18758639

The cytoskeleton regulatory protein hMena (ENAH) is overexpressed in human benign breast lesions with high risk of transformation and human epidermal growth factor receptor-2-positive/hormonal receptor-negative tumors., PMID:16533770

Critical role of Ena/VASP proteins for filopodia formation in neurons and in function downstream of netrin-1., PMID:15066263

Human Mena protein, a serex-defined antigen overexpressed in breast cancer eliciting both humoral and CD8+ T-cell immune response., PMID:15027125

IRSp53 is colocalised with WAVE2 at the tips of protruding lamellipodia and filopodia independently of Mena., PMID:12734400

N-WASP, WAVE and Mena play different roles in the organization of actin cytoskeleton in lamellipodia., PMID:11282031

cAMP-dependent protein kinase phosphorylation of EVL, a Mena/VASP relative, regulates its interaction with actin and SH3 domains., PMID:10945997

Directed actin polymerization is the driving force for epithelial cell-cell adhesion., PMID:10660044

Affinity mass spectrometry-based approaches for the analysis of protein-protein interaction and complex mixtures of peptide-ligands., PMID:10552900

Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics., PMID:8861907

Datasheet

Document Download

Anti-ENAH Antibody (R4C59).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-ENAH Antibody (R4C59) [RHP03401]
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