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Anti-WIF1 Antibody (R4A23)

Catalog #:   RHN58202 Specific References (30) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IF, IHC, WB
Accession: Q9Y5W5
Overview

Catalog No.

RHN58202

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

WIF1, WIF-1, Wnt inhibitory factor 1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9Y5W5

Applications

IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R4A23

Data Image
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using WIF1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human ovary tumour tissues (left) and lung cancer (right) using WIF1 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.
References

Oligodendroglial precursor cells modulate immune response and early demyelination in a murine model of multiple sclerosis., PMID:40173259

Mapping RANKL- and OPG-expressing cells in bone tissue: the bone surface cells as activators of osteoclastogenesis and promoters of the denosumab rebound effect., PMID:39424806

Identification and validation of plasma biomarkers for diagnosis of breast cancer in South Asian women., PMID:34997107

Scl-Ab reverts pro-osteoclastogenic signalling and resorption in estrogen deficient osteocytes., PMID:33148174

Evolution of RAS Mutational Status in Liquid Biopsies During First-Line Chemotherapy for Metastatic Colorectal Cancer., PMID:32766143

RORA Overexpression Alleviates Nasal Mucosal Injury and Enhances Red Blood Cell Immune Adhesion Function in a Mouse Model of Allergic Rhinitis via Inactivation of the Wnt/β-Catenin Signaling Pathway., PMID:31340215

Aberrant Wnt signaling in multiple myeloma: molecular mechanisms and targeting options., PMID:30770859

Exploring pathways for sustained melanogenesis in facial melasma: an immunofluorescence study., PMID:29846953

RAS mutation analysis in circulating tumor DNA from patients with metastatic colorectal cancer: the AGEO RASANC prospective multicenter study., PMID:29438522

Dampening of the bone formation response following repeat dosing with sclerostin antibody in mice is associated with up-regulation of Wnt antagonists., PMID:29129759

Isolating RNA from precursor and mature melanocytes from human vitiligo and normal skin using laser capture microdissection., PMID:27193292

Ornithine Decarboxylase Activity Is Required for Prostatic Budding in the Developing Mouse Prostate., PMID:26426536

Oxidative DNA damage induces the ATM-mediated transcriptional suppression of the Wnt inhibitor WIF-1 in systemic sclerosis and fibrosis., PMID:25185156

Wnt modulators in the aqueous humor are associated with outer retinal damage severity in patients with neovascular age-related macular degeneration., PMID:25034605

Neural stem cells inhibit melanin production by activation of Wnt inhibitors., PMID:24016750

Promoter methylation of WNT inhibitory factor-1 and expression pattern of WNT/β-catenin pathway in human astrocytoma: pathologic and prognostic correlations., PMID:23328978

CpG island methylator phenotype and Helicobacter pylori infection associated with gastric cancer., PMID:23049225

CpG island methylator phenotype in plasma is associated with hepatocellular carcinoma prognosis., PMID:22180715

[Myeloma bone disease and RANKL signaling]., PMID:21814021

Epigenetic markers for early detection of nasopharyngeal carcinoma in a high risk population., PMID:21535891

Aberrant PLAG1 expression in pleomorphic adenomas of the salivary gland: a molecular genetic and immunohistochemical study., PMID:21394649

Acute myeloid leukemia is characterized by Wnt pathway inhibitor promoter hypermethylation., PMID:20795789

The Wnt inhibitory factor 1 restoration in prostate cancer cells was associated with reduced tumor growth, decreased capacity of cell migration and invasion and a reversal of epithelial to mesenchymal transition., PMID:20573255

Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma., PMID:19690140

Conditional mutations of beta-catenin and APC reveal roles for canonical Wnt signaling in lens differentiation., PMID:19515997

Target genes of the WNT/beta-catenin pathway in Wilms tumors., PMID:16575872

Blockade of Wnt-1 signaling induces apoptosis in human colorectal cancer cells containing downstream mutations., PMID:15735684

The extracellular matrix component WIF-1 is expressed during, and can modulate, retinal development., PMID:15555925

WIF1, a component of the Wnt pathway, is down-regulated in prostate, breast, lung, and bladder cancer., PMID:14517837

A new secreted protein that binds to Wnt proteins and inhibits their activities., PMID:10201374

Datasheet

Document Download

Anti-WIF1 Antibody (R4A23).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-WIF1 Antibody (R4A23) [RHN58202]
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