Anti-HLA-F Antibody (R3Z74)

Catalog No.

RHM00502

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, WB: 1:500-1:2000

Target

Leukocyte antigen F, CDA12, MHC class I antigen F, HLA F antigen, HLA-F, HLAF, HLA class I histocompatibility antigen, alpha chain F, HLA-5.4

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P30511

Applications

ELISA, FCM, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3Z74

Epigenetic age acceleration and methylation differences in IgG4-related cholangitis and primary sclerosing cholangitis., PMID:39819503

Mutated Adenovirus Attacks in West Bengal, India: Risk Evaluation of Multi-Country Outbreaks and Mitigation Strategies., PMID:39428960

Diversity in the HLA-I Recognition of HLA-F Monoclonal Antibodies: HLA-F or HLA-Ib Monospecific, HLA-E or HLA-G Bispecific Antibodies with or without HLA-Ia Reactivity., PMID:38390869

Antibodies for β2-Microglobulin and the Heavy Chains of HLA-E, HLA-F, and HLA-G Reflect the HLA-Variants on Activated Immune Cells and Phases of Disease Progression in Rheumatoid Arthritis Patients under Treatment., PMID:37092447

Quantitative proteomics of differentiated primary bronchial epithelial cells from chronic obstructive pulmonary disease and control identifies potential novel host factors post-influenza A virus infection., PMID:36713229

Sib-pair subgroup familial type 1 diabetes mellitus in children in the state of Qatar., PMID:35802651

Ramifications of the HLA-I Allelic Reactivity of Anti-HLA-E*01:01 and Anti-HLA-E*01:03 Heavy Chain Monoclonal Antibodies in Comparison with Anti-HLA-I IgG Reactivity in Non-Alloimmunized Males, Melanoma-Vaccine Recipients, and End-Stage Renal Disease Patients., PMID:35323192

Four Faces of Cell-Surface HLA Class-I: Their Antigenic and Immunogenic Divergence Generating Novel Targets for Vaccines., PMID:35214796

Immune-related genes and gene sets for predicting the response to anti-programmed death 1 therapy in patients with primary or metastatic non-small cell lung cancer., PMID:34084219

Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis., PMID:33867844

Maternal HLA Ib Polymorphisms in Pregnancy Allo-Immunization., PMID:33859649

Human leukocyte antigen (HLA)-F and -G gene polymorphisms and haplotypes are associated with malaria susceptibility in the Beninese Toffin children., PMID:33781967

The FAT10 post-translational modification is involved in the lytic replication of Kaposi's sarcoma-associated herpesvirus., PMID:33627385

HLA-G and HLA-F protein isoform expression in breast cancer patients receiving neoadjuvant treatment., PMID:32978482

Human Amnion Epithelial Cells Impair T Cell Proliferation: The Role of HLA-G and HLA-E Molecules., PMID:32961693

HLA-F on Autologous HIV-Infected Cells Activates Primary NK Cells Expressing the Activating Killer Immunoglobulin-Like Receptor KIR3DS1., PMID:31270222

European Patent in Immunoncology: From Immunological Principles of Implantation to Cancer Treatment., PMID:31013867

Preimplantation Factor (PIF) Promotes HLA-G, -E, -F, -C Expression in JEG-3 Choriocarcinoma Cells and Endogenous Progesterone Activity., PMID:29073617

The Role of HLA-Class I Heavy-Chain Interactions with Killer-Cell Immunoglobulin-Like Receptors in Immune Regulation., PMID:28008807

Improved Protection in a Rabbit Model of Community-Associated Methicillin-Resistant Staphylococcus aureus Necrotizing Pneumonia upon Neutralization of Leukocidins in Addition to Alpha-Hemolysin., PMID:27527081

Islet cell hyperexpression of HLA class I antigens: a defining feature in type 1 diabetes., PMID:27506584

Serum antibodies to human leucocyte antigen (HLA)-E, HLA-F and HLA-G in patients with systemic lupus erythematosus (SLE) during disease flares: Clinical relevance of HLA-F autoantibodies., PMID:26440212

Monoclonal antibodies to HLA-E bind epitopes carried by unfolded β2 m-free heavy chains., PMID:25982269

Evaluation and integration of genetic signature for prediction risk of nasopharyngeal carcinoma in Southern China., PMID:25180181

Behçet disease-associated MHC class I residues implicate antigen binding and regulation of cell-mediated cytotoxicity., PMID:24821759

Quantified colocalization reveals heterotypic histocompatibility class I antigen associations on trophoblast cell membranes: relevance for human pregnancy., PMID:24006284

Human umbilical cord Wharton's jelly stem cells: immune property genes assay and effect of transplantation on the immune cells of heart failure patients., PMID:22546369

Anti-HLA-E mAb 3D12 mimics MEM-E/02 in binding to HLA-B and HLA-C alleles: Web-tools validate the immunogenic epitopes of HLA-E recognized by the antibodies., PMID:21145594

HLA-F complex without peptide binds to MHC class I protein in the open conformer form., PMID:20483783

Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation., PMID:17179229

The surface expression of HLA-F on decidual trophoblasts increases from mid to term gestation., PMID:16806485

Detection of anti-HLA-F antibodies in sera from cancer patients., PMID:15515436

HLA-F surface expression on B cell and monocyte cell lines is partially independent from tapasin and completely independent from TAP., PMID:14607927

Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition., PMID:12874228

Soluble HLA class I molecules/CD8 ligation trigger apoptosis of CD8+ cells by Fas/Fas-ligand interaction., PMID:12806026

Soluble HLA class I induces NK cell apoptosis upon the engagement of killer-activating HLA class I receptors through FasL-Fas interaction., PMID:12393468

Gamma-globulins prepared from sera of multiparous women bind anti-HLA antibodies and inhibit an established in vivo human alloimmune response., PMID:12130522

Classification and genetic features of neonatal haemochromatosis: a study of 27 affected pedigrees and molecular analysis of genes implicated in iron metabolism., PMID:11546828

Functional characterization of HLA-F and binding of HLA-F tetramers to ILT2 and ILT4 receptors., PMID:11169396

HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression., PMID:11137224

The [HLA-B18, F1C30, DR3] conserved extended haplotype carries a susceptibility gene for IgD deficiency., PMID:10941830

Analysis of HLA class Ib gene expression in male gametogenic cells., PMID:9247579

Expression of HLA class I genes in meiotic and post-meiotic human spermatogenic cells., PMID:8793064

Evidence for the expression of non-HLA-A,-B,-C class I genes in the human fetal liver., PMID:1624808

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