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Anti-AMACR Antibody (R3Y29)

Catalog #:   RHJ99801 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: IF, IHC, WB
Accession: Q9UHK6
Overview

Catalog No.

RHJ99801

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

AMACR, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9UHK6

Applications

IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3Y29

Data Image
  • Immunofluorescence
    Confocal Immunofluorescence analysis of LNCaP cells using AMACR mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using AMACR mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human brain cerebellum using AMACR mouse mAb.
  • Western blot
    Western blot analysis using AMACR mouse mAb against Jurkat (1) and LNCaP (2) cell lysate.
References

Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67., PMID:39388614

Multiplexed Opto-Microfluidic Biosensing: Advanced Platform for Prostate Cancer Detection., PMID:38683677

Immunohistochemical Expression of Alpha-Methyl-Coa (AMACR) and ERG in Prostatic Adenocarcinoma and Prostatic Hyperplasia: A Comparative Study., PMID:37642075

Autoantibody Positivity in Two Bahraini Siblings With a Novel Alpha-Methylacyl-CoA Racemase Mutation., PMID:37575761

Utility of AMACR immunohistochemical staining in differentiating Arias-Stella reaction from clear cell carcinoma of ovary and endometrium., PMID:37041497

Sustained release of alpha-methylacyl-CoA racemase (AMACR) antibody-conjugated and free doxorubicin from silica nanoparticles for prostate cancer cell growth inhibition., PMID:36314600

Immunoexpression of oral brush biopsy enhances the accuracy of diagnosis for oral lichen planus and lichenoid lesions., PMID:35460123

Performance comparison of anti-p504s (SP116) Rabbit Monoclonal Primary Antibody vs. Monoclonal Rabbit Anti-Human AMACR clone 13H4 when duplexed with VENTANA Basal Cell Cocktail (34βE12+p63) as a diagnostic aid for prostatic adenocarcinoma using immunohistochemistry., PMID:33811532

Intestinal, intestinal-type and intestine-localized metastatic adenocarcinoma. Immunohistochemical approach to the differential diagnosis., PMID:33336735

Serum total prostate-specific antigen (tPSA): correlation with diagnosis and grading of prostate cancer in core needle biopsy., PMID:32815673

Comprehensive Immunoprofiles of Renal Cell Carcinoma Subtypes., PMID:32150988

Immunohistochemical Reactivity of Prostate-Specific Markers for Salivary Duct Carcinoma., PMID:31865345

Diagnostic utility of triple antibody (AMACR, HMWCK and P63) stain in prostate neoplasm., PMID:31548949

Loss of PTEN Expression Detected by Fluorescence Immunohistochemistry Predicts Lethal Prostate Cancer in Men Treated with Prostatectomy., PMID:31411988

Signature maps for automatic identification of prostate cancer from colorimetric analysis of H&E- and IHC-stained histopathological specimens., PMID:31061447

Papillary renal cell carcinoma with cytologic and molecular genetic features overlapping with renal oncocytoma: Analysis of 10 cases., PMID:30072012

34BetaE12 and Alfa-Methylacyl Coenzyme A Racemase (AMACR) Antibodies Better Than p63 Antibody Distinguish Normal and Neoplastic Glands in Prostatic Tissue With Thermal Artifacts., PMID:29346181

Preparation and characterization of the antibody recognizing AMACR inside its catalytic center., PMID:29345292

Vasitis nodosa and related lesions: a modern immunohistochemical staining profile with special emphasis on novel diagnostic dilemmas., PMID:29241741

Typing the atypical: Diagnostic issues and predictive markers in suspicious prostate lesions., PMID:28828885

Immunohistochemical Profile of Tumor Pathways and Prognostic Significance in Colon Adenocarcinomas., PMID:28605329

[Fatty acid synthase in the diagnosis of prostate neoplasms]., PMID:28418352

Diagnostic Value of ERG in Prostate Needle Biopsies Containing Minute Cancer Foci., PMID:28384107

A Novel Monoclonal Antibody Against Alpha-Methylacyl-CoA Racemase Applicable for Paraffin-Embedded Tissues and Diagnostics of Prostate Cancer., PMID:28234557

[Estimation of the diagnostic potential of APOD, PTOV1, and EPHA4 for prostatic neoplasms]., PMID:27804940

Immunohistochemistry in the diagnosis of dysplasia in chronic inflammatory bowel disease colorectal polyps., PMID:27567189

Same difference: A pilot study of cyclin D1, bcl-2, AMACR, and ALDH-1 identifies significant differences in expression between primary colon adenocarcinoma and its metastases., PMID:27623206

Lack of ERG-antibody in Benign Mimickers of Prostate Cancer., PMID:27383878

Quantitative Time-Resolved Fluorescence Imaging of Androgen Receptor and Prostate-Specific Antigen in Prostate Tissue Sections., PMID:27026295

Horseradish peroxidase and aptamer dual-functionalized nanoprobe for the amplification detection of alpha-methylacyl-CoA racemase., PMID:26547498

Tubulocystic renal cell carcinoma is an entity that is immunohistochemically and genetically distinct from papillary renal cell carcinoma., PMID:26310887

Ductal and Acinar Adenocarcinoma of Prostate: Morphological and Immunohistochemical Characterization., PMID:26171121

Analytical platform evaluation for quantification of ERG in prostate cancer using protein and mRNA detection methods., PMID:25889691

Application of combined immunohistochemical panel of AMACR(P504S)/p63 cocktail, cytokeratin 5 and D2-40 to atypical glands in prostatic needle biopsy., PMID:25500515

Diagnostic utility of p63 and α-methyl acyl Co A racemase in resolving suspicious foci in prostatic needle biopsy and transurethral resection of prostate specimens., PMID:25313761

Best practices recommendations in the application of immunohistochemistry in the prostate: report from the International Society of Urologic Pathology consensus conference., PMID:25029122

Immunohistochemical staining characteristics of nephrogenic adenoma using the PIN-4 cocktail (p63, AMACR, and CK903) and GATA-3., PMID:24921643

[Three-year results of the treatment of localized prostate cancer using high-intensity focused ultrasound]., PMID:24772773

Observations on Aron et al's "Utility of a triple antibody cocktail intraurothelial neoplasm-3 (IUN-3 CK20/CD44s/p53) and α-methylacyl-CoA racemase (AMACR) in the distinction of urothelial carcinoma in situ (CIS) and reactive urothelial atypia"., PMID:24618606

Molecular subtyping of metastatic renal cell carcinoma: implications for targeted therapy., PMID:24568263

Utility of a triple antibody cocktail intraurothelial neoplasm-3 (IUN-3-CK20/CD44s/p53) and α-methylacyl-CoA racemase (AMACR) in the distinction of urothelial carcinoma in situ (CIS) and reactive urothelial atypia., PMID:24225842

Nephrogenic adenoma of the urinary tract: clinical, histological, and immunohistochemical characteristics., PMID:24142157

[Pathological and immunohistochemical analyses of 32 cases of nephrogenic adenoma]., PMID:23939154

Comparative analysis of three- and two-antibody cocktails to AMACR and basal cell markers for the immunohistochemical diagnosis of prostate carcinoma., PMID:22800084

Nephrogenic adenoma: an immunohistochemical analysis using biotin-free methods., PMID:22495376

Nephrogenic adenoma of the urinary tract: a review., PMID:22415059

The value of triple antibody (34βE12 + p63 + AMACR) cocktail stain in radical prostatectomy specimens with crushed surgical margins., PMID:22294716

p63, CK7, PAX8 and INI-1: an optimal immunohistochemical panel to distinguish poorly differentiated urothelial cell carcinoma from high-grade tumours of the renal collecting system., PMID:22260386

Mn-doped ZnSe d-dots-based α-methylacyl-CoA racemase probe for human prostate cancer cell imaging., PMID:22241581

[Alternative tests to PSA for prostate cancer diagnosis]., PMID:21553390

Datasheet

Document Download

Anti-AMACR Antibody (R3Y29).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-AMACR Antibody (R3Y29) [RHJ99801]
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