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Anti-NANOG Antibody (R3W58)

Catalog #:   RHJ58301 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IF, WB
Accession: Q9H9S0
Overview

Catalog No.

RHJ58301

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IF: 1:200-1:1000, WB: 1:500-1:2000

Target

Homeobox protein NANOG, NANOG, Homeobox transcription factor Nanog, hNanog

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9H9S0

Applications

IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3W58

Data Image
  • Immunofluorescence
    Confocal Immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
  • Western blot
    Western blot analysis using NANOG mouse mAb against NTERA-2 cell lysate (2).
References

Pluripotent stem cell marker deficiency in salivary mucoepidermoid carcinoma with relevance to molecular profiling: An experimental study., PMID:40478494

A dual-Color closed bipolar electrochemiluminescence platform for visual simultaneous diagnosis of two pluripotency markers in cancer patients' urine., PMID:40253801

Green emitting carbon dots-immunosensor on magnetic nanoparticles for detection of Nanog antigen as a cancer stem cell biomarker., PMID:40221207

Hedgehog Pathway Is a Regulator of Stemness in HER2-Positive Trastuzumab-Resistant Breast Cancer., PMID:39596169

Prediction of synergistic gemcitabine-based combination treatment through a novel tumor stemness biomarker NANOG in pancreatic cancer., PMID:39297058

Morphological and Immunocytochemical Characterization of Paclitaxel-Induced Microcells in Sk-Mel-28 Melanoma Cells., PMID:39062149

CRET-based immunoassay on magnetic beads for selective and sensitive detection of Nanog antigen as a key cancer stem cell marker., PMID:38916771

Blastocyst-like Structures in the Peripheral Retina of Young Adult Beagles., PMID:38892233

The HSP90 inhibitor HVH-2930 exhibits potent efficacy against trastuzumab-resistant HER2-positive breast cancer., PMID:38646654

Prostate cancer research: tools, cell types, and molecular targets., PMID:38595814

Targeting the glycan epitope type I N-acetyllactosamine enables immunodepletion of human pluripotent stem cells from early differentiated cells., PMID:38349796

Successful xenotransplantation of testicular cells following fractionated chemotherapy of recipient birds., PMID:38321093

A short history of pluripotent stem cells markers., PMID:38157849

Heterogeneity of nucleolar morphology in four-cell mouse embryos after IVF: association with developmental potential., PMID:38102887

β-CATENIN stabilizes HIF2 through lncRNA and inhibits intravenous immunoglobulin immunotherapy., PMID:37744383

The lncRNA expression profile signature of leukemia stem cells is altered upon PI3K/mTOR inhibition: an in vitro and in silico study., PMID:37470414

Analysis of N-glycosylation protein of Kashin-Beck disease chondrocytes derived from induced pluripotent stem cells based on label-free strategies with LC-MS/MS., PMID:37186116

Elucidating the immunohistochemistry of Nanog: A transcription marker in the oral squamous cell carcinoma with emphasis on its origin as embryonic stem cell., PMID:37082043

Treatment with decitabine induces the expression of stemness markers, PD-L1 and NY-ESO-1 in colorectal cancer: potential for combined chemoimmunotherapy., PMID:37004094

IL-6 promotes pluripotency of mouse embryonic stem cells and regulates cardiac differentiation in a development-dependent manner., PMID:36859834

Application of Micro-Western Array for Identifying Different Serum Protein Expression Profile among Healthy Control, Alzheimer's Disease Patients and Patients' Adult Children., PMID:36138870

Preventive cancer stem cell-based vaccination modulates tumor development in syngeneic colon adenocarcinoma murine model., PMID:36040667

A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability., PMID:35975322

Isolation of Swine Bone Marrow Lin-/CD45-/CD133 + Cells and Cardio-protective Effects of its Exosomes., PMID:35925437

Lung Cancer Stem Cell Markers as Therapeutic Targets: An Update on Signaling Pathways and Therapies., PMID:35719973

Oncofetal proteins and cancer stem cells., PMID:35670043

NANOG confers resistance to complement-dependent cytotoxicity in immune-edited tumor cells through up-regulating CD59., PMID:35606403

Thymoquinone-Mediated Modulation of Toll-like Receptors and Pluripotency Factors in Gingival Mesenchymal Stem/Progenitor Cells., PMID:35563755

Sex Influences Age-Related Changes in Natural Antibodies and CD5+ B-1 Cells., PMID:35256511

Characterization of nanog in Nile tilapia (Oreochromis niloticus) and its spatiotemporal expression patterns during embryonic and gonadal development., PMID:35093560

Antibody-free profiling of transcription factor occupancy during early embryogenesis by FitCUT&RUN., PMID:34965941

Cancer-Associated Fibroblasts Regulate the Plasticity of Breast Cancer Stemness through the Production of Leukemia Inhibitory Factor., PMID:34947829

CHAF1b, chromatin assembly factor-1 subunit b, is essential for mouse preimplantation embryos., PMID:34906611

Monoclonal antibody K312-based depletion of pluripotent cells from differentiated stem cell progeny prevents teratoma formation., PMID:34674794

Use of alginate hydrogel to improve long-term 3D culture of spermatogonial stem cells: stemness gene expression and structural features., PMID:34641993

Telocytes in the human ascending aorta: Characterization and exosome-related KLF-4/VEGF-A expression., PMID:34562312

DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification., PMID:34255976

ZEB2 facilitates peritoneal metastasis by regulating the invasiveness and tumorigenesis of cancer stem-like cells in high-grade serous ovarian cancers., PMID:34211089

MUSASHI-2 confers resistance to third-generation EGFR-tyrosine kinase inhibitor osimertinib in lung adenocarcinoma., PMID:34145929

CD44 loss of function sensitizes AML cells to the BCL-2 inhibitor venetoclax by decreasing CXCL12-driven survival cues., PMID:34115113

Germ line polymorphisms of genes involved in pluripotency transcription factors predict efficacy of cetuximab in metastatic colorectal cancer., PMID:33901792

Phenotypical Characterization and Neurogenic Differentiation of Rabbit Adipose Tissue-Derived Mesenchymal Stem Cells., PMID:33802902

PI3K/Akt pathway and Nanog maintain cancer stem cells in sarcomas., PMID:33468992

Generation of Systemic Lupus Erythematosus Patient-Derived Induced Pluripotent Stem Cells from Blood., PMID:33397195

A Comparison of Methods for Isolation of Limbal Niche Cells: Maintenance of Limbal Epithelial Stem/Progenitor Cells., PMID:33320169

Molecular phenotypes of circulating tumor cells and efficacy of nivolumab treatment in patients with head and neck squamous cell carcinoma., PMID:33299117

Glioma Stem-Like Cells Can Be Targeted in Boron Neutron Capture Therapy with Boronophenylalanine., PMID:33086625

SOSTDC1 promotes invasion and liver metastasis in colorectal cancer via interaction with ALCAM/CD166., PMID:32801337

Spontaneous healing of human amnion in the premature rupture of membrane model., PMID:32792059

NANOG as prognostic factor of prostate cancer course., PMID:32602285

Datasheet

Document Download

Anti-NANOG Antibody (R3W58).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-NANOG Antibody (R3W58) [RHJ58301]
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